Clustering and geolocation of ocean samples based on SAR11 phylotype composition. Clustering and geolocation of ocean samples based on SAR11 phylotype.

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Clustering and geolocation of ocean samples based on SAR11 phylotype composition. Clustering and geolocation of ocean samples based on SAR11 phylotype composition. (A) dbRDA ordination for the fitted model of SAR11 ITS phylotype composition data (based on Bray‐Curtis similarity after square root transformation of abundances) versus environmental variables. Clustering illustrates the strong separation of samples related to temperature bins <10°C (open circles), 10–20°C (black triangles) and >20°C (grey squares). (B) Identical plot to (A), with sample icons changed to reflect the ‘coastal’ (grey triangles) or ‘open ocean’ (open squares) affiliation of each sample. (C) Eigen vectors indicating the strength and direction of correlation for each environmental variable from (A). (D) Eigen vectors indicating the strength and direction of correlation for each SAR11 phylotype from (A). (E) nMDS based on between sample Bray‐Curtis similarities calculated using square root transformed SAR11 community composition and abundance data (2D stress: 0.07). Clusters are coloured based on membership to groups identified as having >60% similarity in community composition (marked by green rings). Group number (1) blue diamonds, samples from polar regions; (2) light blue squares, temperate regions; (3) red triangles, tropical regions; (4) green triangles, Western Channel Observatory (UK), Monterey Bay (USA) and Botany Bay (Australia); (5) fuschia dots, Chesapeake Bay, Delaware Bay and nearby Nags Head; (6) black ‘x’, GS037 and S_35139 (tropical Pacific Ocean) and NASB_179_2 and NASB_174_2 (Sargasso Sea) (contain only ‘tropical’ phylotype P1a.3); (7) grey cross, Mont_bay_3 (contains only phylotype P2.1); (8) pink square, NASB‐179_1 (Sargasso Sea) (contains only phylotype P1b). (F) Geolocation of the groups shown in (E), depicted by stars (as coloured in E). Mark V Brown et al. Mol Syst Biol 2012;8:595 © as stated in the article, figure or figure legend