Cardiovascular and metabolic profiles of offspring conceived by assisted reproductive technologies: a systematic review and meta-analysis Xiao-Yan Guo,

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Cardiovascular and metabolic profiles of offspring conceived by assisted reproductive technologies: a systematic review and meta-analysis Xiao-Yan Guo, M.D., Xin-Mei Liu, Ph.D., Li Jin, Ph.D., Ting-Ting Wang, Ph.D., Kamran Ullah, Ph.D., Jian-Zhong Sheng, M.D., Ph.D., He- Feng Huang, M.D. Fertility and Sterility Volume 107, Issue 3, Pages 622-631.e5 (March 2017) DOI: 10.1016/j.fertnstert.2016.12.007 Copyright © 2016 American Society for Reproductive Medicine Terms and Conditions

Figure 1 Systolic and diastolic blood pressure of 872 IVF-ICSI offspring versus 3,034 naturally conceived offspring. Studies are arranged and grouped by mean birth year of the children. Random effects model was used. CI = confidence interval; DBP = systolic diastolic blood pressure; SBP = systolic blood pressure; WMD = weighted mean difference. Fertility and Sterility 2017 107, 622-631.e5DOI: (10.1016/j.fertnstert.2016.12.007) Copyright © 2016 American Society for Reproductive Medicine Terms and Conditions

Figure 2 Body mass index of 1,914 IVF-ICSI offspring versus 3,881 naturally conceived offspring. Studies are arranged according to mean age of the subjects. Random effects model was used. CI = confidence interval; WMD = weighted mean difference. Fertility and Sterility 2017 107, 622-631.e5DOI: (10.1016/j.fertnstert.2016.12.007) Copyright © 2016 American Society for Reproductive Medicine Terms and Conditions

Figure 3 (A) Glucose homeostasis variables and (B) lipid profiles of 477 IVF-ICSI offspring versus 1,852 naturally conceived offspring. Studies are arranged by first author. Random or fixed effects model was used according to the heterogeneity. CI = confidence interval; HDL = high-density lipoprotein; HOMA = homeostasis model of assessment; LDL = low-density lipoprotein; WMD = weighted mean difference. Fertility and Sterility 2017 107, 622-631.e5DOI: (10.1016/j.fertnstert.2016.12.007) Copyright © 2016 American Society for Reproductive Medicine Terms and Conditions

Supplemental Figure 1 PRISMA flowchart of literature search process. NC = naturally conceived offspring; OI = ovulation induction; PGD = preimplantation genetic diagnosis. Fertility and Sterility 2017 107, 622-631.e5DOI: (10.1016/j.fertnstert.2016.12.007) Copyright © 2016 American Society for Reproductive Medicine Terms and Conditions

Supplemental Figure 2 Influence analyses of (A) systolic blood pressure and (B) diastolic blood pressure. CI = confidence interval. Fertility and Sterility 2017 107, 622-631.e5DOI: (10.1016/j.fertnstert.2016.12.007) Copyright © 2016 American Society for Reproductive Medicine Terms and Conditions