A nested polymerase chain reaction for detection of Legionella pneumophila in clinical specimens Sverker Bernander, Hanna-Stina Hanson, Bo Johansson,

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A nested polymerase chain reaction for detection of Legionella pneumophila in clinical specimens Sverker Bernander, Hanna-Stina Hanson, Bo Johansson, Lars-Victor von Stedingk Clinical Microbiology and Infection Volume 3, Issue 1, Pages 95-101 (February 1997) DOI: 10.1111/j.1469-0691.1997.tb00257.x Copyright © 1997 European Society of Clinical Infectious Diseases Terms and Conditions

Fig. 1 Agarose gel electrophoresis of nested PCR products. Lane 1: DNA molecular weight marker VIII (Boehringer Mannheim GmbH, Mannheim, Germany). Lanes 2, 4 and 6: three aliquots from the same DNA preparation of a sputum sample from a patient with Legionella infection. Lanes 3, 5, 7 and 10 are negative controls. Lane 8: a negative sputum. Lane 9: the same sample as in lane 8, but with 50 CFU of L. pneumophila sg 1 (ATCC 33152) added. Lane 11: positive control of heat-lysed L. pneumophila sg 1 (ATCC 33152). Clinical Microbiology and Infection 1997 3, 95-101DOI: (10.1111/j.1469-0691.1997.tb00257.x) Copyright © 1997 European Society of Clinical Infectious Diseases Terms and Conditions