Loop-mediated isothermal amplification assay for the diagnosis of retinitis caused by herpes simplex virus-1 A.K. Reddy, P.K. Balne, R.K. Reddy, A. Mathai,

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Presentation transcript:

Loop-mediated isothermal amplification assay for the diagnosis of retinitis caused by herpes simplex virus-1 A.K. Reddy, P.K. Balne, R.K. Reddy, A. Mathai, I. Kaur Clinical Microbiology and Infection Volume 17, Issue 2, Pages 210-213 (February 2011) DOI: 10.1111/j.1469-0691.2010.03216.x Copyright © 2011 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 1 Electrophoretic analysis of loop-mediated isothermal amplification (LAMP) products. Lane 1, 100 bp DNA ladder. Lane 2, negative control; lanes 3 and 4, herpes simplex virus (HSV)-1 LAMP products; lanes 5, 6, 7, 8, 9 and 10, no LAMP product with other organisms (HSV-2, cytomegalovirus, varicella zoster virus, adenovirus, Aspergillus flavus and Staphylococcus aureus). Clinical Microbiology and Infection 2011 17, 210-213DOI: (10.1111/j.1469-0691.2010.03216.x) Copyright © 2011 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 2 Visual detection of loop-mediated isothermal amplification products. The tube with a positive reaction (tube 1) shows a yellowish–green colour change, which can be distinguished from the reddish–orange colour of a negative reaction (tube 2). Clinical Microbiology and Infection 2011 17, 210-213DOI: (10.1111/j.1469-0691.2010.03216.x) Copyright © 2011 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 3 Sequence alignment of the herpes simplex virus 1 (HSV-1) positive loop-mediated isothermal amplification product (Red) with deposited sequence of HSV-1 (Black) in the GenBank (D00373). Clinical Microbiology and Infection 2011 17, 210-213DOI: (10.1111/j.1469-0691.2010.03216.x) Copyright © 2011 European Society of Clinical Infectious Diseases Terms and Conditions