Lung mast cells are a source of secreted phospholipases A2

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Presentation transcript:

Lung mast cells are a source of secreted phospholipases A2 Massimo Triggiani, MD, PhD, Giorgio Giannattasio, MD, Cecilia Calabrese, MD, PhD, Stefania Loffredo, PhD, Francescopaolo Granata, MD, PhD, Alfonso Fiorello, MD, Mario Santini, MD, Michael H. Gelb, PhD, Gianni Marone, MD, FRCP Journal of Allergy and Clinical Immunology Volume 124, Issue 3, Pages 558-565.e3 (September 2009) DOI: 10.1016/j.jaci.2009.04.035 Copyright © 2009 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 1 Characterization of PLA2 activity in human BALF. A, BALF from control subjects (n = 19) and asthmatic patients (n = 14) was assayed for PLA2 activity, as described in the Methods section. Boxes indicate means ± SEs. B, BALF was preincubated (for 1 hour at 37°C) with 10 mmol/L DTT, 10 μmol/L Me-Indoxam, 10 μmol/L AZ-1, or 2 mmol/L PMSF before the PLA2 activity assay. Data are presented as means ± SEs of BALF from 4 asthmatic patients. ∗P < .01 versus untreated. Journal of Allergy and Clinical Immunology 2009 124, 558-565.e3DOI: (10.1016/j.jaci.2009.04.035) Copyright © 2009 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 2 Characterization of PLA2 activity in supernatants of HLMCs. A, HLMCs were incubated (for 30 minutes at 37°C) with anti-IgE. PLA2 activity was determined in supernatants, as described in the Methods section. Data are presented as means ± SEs of 4 experiments. ∗P < .05 versus unstimulated; ∗∗P < .01 versus unstimulated. B, Supernatants of anti-IgE–stimulated HLMCs were preincubated (for 1 hour at 37°C) with 10 mmol/L DTT, 10 μmol/L Me-Indoxam, 10 μmol/L AZ-1, or 2 mmol/L PMSF before the PLA2 activity assay. Data are presented as means ± SEs of 4 experiments. ∗P < .01 versus untreated. Journal of Allergy and Clinical Immunology 2009 124, 558-565.e3DOI: (10.1016/j.jaci.2009.04.035) Copyright © 2009 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 3 Release of sPLA2s and histamine from anti-IgE–stimulated HLMCs. A, HLMCs were incubated with or without anti-IgE (1 μg/mL). Histamine release and PLA2 activity were determined in supernatants, as described in the Methods section. Data are presented as means ± SEs of 4 experiments. ∗P < .01 versus unstimulated. B, HLMCs were incubated (for 30 minutes at 37°C) with anti-IgE (1 μg/mL). Histamine release and PLA2 activity were determined as described above. Correlation between histamine release and sPLA2 activity was assessed by using the linear regression function of Microsoft Excel. Journal of Allergy and Clinical Immunology 2009 124, 558-565.e3DOI: (10.1016/j.jaci.2009.04.035) Copyright © 2009 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 4 Expression of sPLA2s in HLMCs. HLMCs from 2 different lung preparations were lysed, and total RNA was extracted. Expression of sPLA2s was evaluated by means of semiquantitative PCR, as described in the Methods section. RT-PCR amplification products were separated on 2% agarose gel, stained with ethidium bromide, and photographed. Journal of Allergy and Clinical Immunology 2009 124, 558-565.e3DOI: (10.1016/j.jaci.2009.04.035) Copyright © 2009 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 5 Effect of sPLA2 and cPLA2 inhibitors on LTC4 production from anti-IgE–stimulated HLMCs. The cells were preincubated (for 15 minutes at 37°C) with increasing concentrations of Me-Indoxam or AZ-1 before stimulation (for 30 minutes at 37°C) with anti-IgE (1 μg/mL). LTC4 production was determined in supernatants by using an enzyme immunoassay. Inhibition of LTC4 production was expressed as a percentage of maximum response. Data are presented as means ± SEs of 4 experiments. ∗P < .05 versus anti-IgE; ∗∗P < .01 versus anti-IgE. Journal of Allergy and Clinical Immunology 2009 124, 558-565.e3DOI: (10.1016/j.jaci.2009.04.035) Copyright © 2009 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Correlation between PLA2 activity and protein content in the BALF Correlation between PLA2 activity and protein content in the BALF. Cell-free BALF from patients with bronchial asthma (A; n = 14) and control subjects (B; n = 19) was assayed for PLA2 activity by using tritiated OA–labeled E coli membranes, as described in the Methods section, and for total protein content by using a Bradford-based assay. Data are plotted as a function of PLA2 activity versus protein content. Correlation was assessed by using the linear regression function of Microsoft Excel software. Journal of Allergy and Clinical Immunology 2009 124, 558-565.e3DOI: (10.1016/j.jaci.2009.04.035) Copyright © 2009 American Academy of Allergy, Asthma & Immunology Terms and Conditions