Transport – in / out of Cell Membrane

reactions movement http://www.google.ca/imgres?q=membrane+transport&hl=en&sa=X&rls=com.microsoft:en-us:IE-SearchBox&tbm=isch&prmd=imvnsb&tbnid=OONcuo_AyV0jxM:&imgrefurl=http://en.wikibooks.org/wiki/Human_Physiology/Cell_physiology&docid=JG7EjMv1kau7vM&w=702&h=371&ei=VOmETouyOfPYiAK9-sXQDA&zoom=1&iact=hc&vpx=189&vpy=151&dur=78&hovh=163&hovw=309&tx=173&ty=95&page=5&tbnh=72&tbnw=136&start=32&ndsp=8&ved=1t:429,r:5,s:32&biw=792&bih=454

polar

Membrane Features all some living cell membrane http://www.google.ca/imgres?q=selectively+permeable&hl=en&sa=X&rls=com.microsoft:en-us:IE-SearchBox&tbm=isch&prmd=imvns&tbnid=5N2zjwO4LQV47M:&imgrefurl=http://www.columbia.edu/cu/psychology/courses/1010/mangels/neuro/neurosignaling/neurosignaling.html&docid=F0l6mMXF7d9EyM&w=150&h=150&ei=m_OETs-7EIjQiALr-Oi5DA&zoom=1&iact=hc&vpx=263&vpy=196&dur=2687&hovh=120&hovw=120&tx=64&ty=93&page=1&tbnh=102&tbnw=114&start=0&ndsp=8&ved=1t:429,r:1,s:0&biw=792&bih=454

Water, carbon dioxide oxygen glucose Na+ (sodium), H+ (hydrogen) http://www.google.ca/imgres?q=selective+permeability&hl=en&sa=X&rls=com.microsoft:en-us:IE-SearchBox&tbm=isch&prmd=imvns&tbnid=6ioQOUcU9j5KOM:&imgrefurl=http://environmentsofcells.wikispaces.com/&docid=FcVwGWQ9v5y_fM&w=500&h=379&ei=KPSETvCnDaXZiAKcneGoDA&zoom=1&iact=hc&vpx=210&vpy=77&dur=11188&hovh=195&hovw=258&tx=129&ty=113&page=1&tbnh=114&tbnw=150&start=0&ndsp=7&ved=1t:429,r:1,s:0&biw=792&bih=454

http://www. google. ca/imgres http://www.google.ca/imgres?q=selective+permeability&hl=en&sa=X&rls=com.microsoft:en-us:IE-SearchBox&tbm=isch&prmd=imvns&tbnid=6ioQOUcU9j5KOM:&imgrefurl=http://environmentsofcells.wikispaces.com/&docid=FcVwGWQ9v5y_fM&w=500&h=379&ei=KPSETvCnDaXZiAKcneGoDA&zoom=1&iact=hc&vpx=210&vpy=77&dur=11188&hovh=195&hovw=258&tx=129&ty=113&page=1&tbnh=114&tbnw=150&start=0&ndsp=7&ved=1t:429,r:1,s:0&biw=792&bih=454

http://www.youtube.com/watch?v=GFCcnxgXOhY

Transport Mechanisms

inside outside Random away

https://www. youtube. com/watch https://www.youtube.com/watch?v=MS5igwkWKe8&list=PLLhLoKibENnm9coBiS-HkVq-y992mm4dx&index=9

1. Diffusion (Passive Transport)

REVIEW: http://www.google.ca/imgres?q=solute+solvent+solution&hl=en&sa=X&rls=com.microsoft:en-ca:IE-SearchBox&rlz=1I7TSCA&biw=1280&bih=555&tbm=isch&prmd=imvnsfd&tbnid=ki5mLhGb7aQJPM:&imgrefurl=http://water.me.vccs.edu/courses/env211/lesson8_3.htm&docid=TOb2gi68vEUpnM&w=146&h=170&ei=eBuJTpaFOujciALe4IS6DA&zoom=1&iact=hc&vpx=899&vpy=299&dur=3292&hovh=136&hovw=116&tx=87&ty=107&page=2&tbnh=136&tbnw=116&start=18&ndsp=10&ved=1t:429,r:3,s:18

Process of diffusion When crystals of dye are placed in water, they are concentrated in one area.

energy down increases increases faster selectively

2. Osmosis (Passive Transport)

Osmosis Animation http://www.stolaf.edu/people/giannini/flashanimat/transport/osmosis.swf

Tonicity Summary

Let’s practice …

Practice # 1: Solution B Solution A 6% NaCl 94% H2O 6 % NaCl 94 % H2O Solution A is _isotonic /hypotonic / hypertonic_ to solution B? Solution B is __ _isotonic / hypotonic / hypertonic_ to solution A?

Practice # 2: Solution B Solution A 10% NaCl 90% H2O 2 % NaCl 98 % H2O Solution A is _isotonic /hypotonic / hypertonic_ to solution B? Solution B is __ _isotonic / hypotonic / hypertonic_ to solution A?

Practice # 2:

Net movement of water is from A to B. A is HYPOTONIC to B B is HYPERTONIC to A C is ISOTONIC to D D is ISOTONIC to C

Water will always from an area more water to an area of lower water levels – goal is to reach equilibrim (equal concentrations or isotonicity) http://www.biologycorner.com/bio1/diffusion.html#

Equilibrium: osmotic pressure = hydrostatic pressure. Add protein to side B Equilibrium: osmotic pressure = hydrostatic pressure. Semi-permeable membrane

p 86 in text

p 86 in text

3. Facilitated Transport (Passive Transport)

3. Facilitated Transport (Passive Transport)

Facilitated Diffusion

High to low concentration (down a concentration gradient.) carrier polar hundreds energy

Facilitated Diffusion

very SPECIFIC Facilitated Diffusion : The protein channels and carriers are very SPECIFIC

Combo of VP and M Paas slides Done to here Combo of VP and M Paas slides

Active Transport

ENERGY Carrier (transport) against steep

conformations inside Phosphorylation

positive potential

H+ diffuses in with sucrose. Sucrose can only enter with H+. H+ pumped out H+ diffuses in with sucrose. Sucrose can only enter with H+. http://www.google.ca/imgres?q=sucrose+symport&hl=en&rls=com.microsoft:en-ca:IE-SearchBox&rlz=1I7TSCA&biw=1280&bih=555&tbm=isch&tbnid=Wiz_AyKUWTXfjM:&imgrefurl=http://www.uic.edu/classes/bios/bios100/lecturesf04am/lect19.htm&docid=CFN3b259gj6SfM&w=800&h=600&ei=fjOJTuKJMMjYiALLjM2gDA&zoom=1&iact=hc&vpx=371&vpy=132&dur=4212&hovh=194&hovw=259&tx=127&ty=117&page=1&tbnh=103&tbnw=141&start=0&ndsp=25&ved=1t:429,r:2,s:0

Exocytosis Exocytosis deposits substances on the outside of the cell and secretion occurs.

Phagocytosis Phagocytosis occurs when the substance to be transported into the cell is large; amoebas ingest food by phagocytosis. Certain types of human white blood cells are amoeboid and engulf worn-out cellular debris or bacteria using phagocytosis. When an endocytic vesicle fuses with a lysosome, digestion of the vesicle contents occurs.

Phagocytosis Pseudopods lysosome lysososome

Pinocytosis Pinocytosis occurs when a macromolecule, such as a polypeptide, is to be transported into the cell. The resulting vesicle or vacuole is small. Pinocytosis occurs continuously, but the loss of plasma membrane due to vesicle formation is offset by exocytosis.

Pinocytosis Non-discriminating Non-discriminating

Receptor-mediated endocytosis discriminating Receptor-mediated endocytosis is a form of pinocytosis. The substance to be taken in binds with a specific receptor protein, which migrates to a pit or is already in a coated pit. The resulting vesicle contains the substance and the receptor. Receptor-mediated endocytosis is responsible for cells taking uplow-density lipoprotein (LDL) when LDL receptors gather in a coated pit. In individuals with a genetic disorder called familial hypercholesterolemia, the LDL receptor is unable to properly bind to the coated pit, and cells are unable to take up cholesterol. Cholesterol accumulates in the walls of arterial blood vessels, causing severe health problems.

Receptor-mediated discriminating http://www.google.ca/imgres?q=receptor+mediated+endocytosis&hl=en&sa=X&rls=com.microsoft:en-ca:IE-SearchBox&rlz=1I7TSCA&biw=1280&bih=555&tbm=isch&prmd=imvns&tbnid=aPXY-AkbavvS7M:&imgrefurl=http://www.thefullwiki.org/Receptor-mediated_endocytosis&docid=YsD-5KBkcHtNOM&w=300&h=224&ei=WBqJTu63MuPWiALGr8m7DA&zoom=1&iact=hc&vpx=848&vpy=256&dur=3073&hovh=179&hovw=240&tx=127&ty=138&page=2&tbnh=150&tbnw=201&start=11&ndsp=11&ved=1t:429,r:3,s:11

bulk receptor proteins

Read and make notes on Chapter 4 Read and make notes on Chapter 4. Read critically and take selective notes.

DOWN UP (AGAINST) Energy (ATP) NO added energy Diffusion Osmosis Phagocytosis Active Transport Facilitated Diffusion Pinocytosis

DOWN UP (AGAINST) Energy (ATP) NO added energy Diffusion Osmosis Phagocytosis Active Transport Facilitated Diffusion Pinocytosis