LAB 3 ENZYMATIC DIGESTION

OBJECTIVES BUILDING BLOCKS OF PROTEINS, FATS, AND CARBOHYDRATES ENZYMES RESPONSIBLE FOR THE HYDROLYSIS OF PROTEINS, FATS, AND CARBOHYDRATES TEST USED TO DETERMINE IF HYDROLYSIS OF THESE MOLECULES HAVE OCCURRED.

BIOMOLECULES 4 major classes of biomolecules 1. Carbohydrates 2. Proteins 3. Lipids 4. Nucleic Acid These biomolecules are made by dehydration and they are broken apart by hydrolysis. There is a specific enzyme to catalyze each reaction

PROCESS OF DIGESTION Mechanical digestion – chewing, mixing, and peristalsis Chemical digestion – special enzymes Hydrolysis – this is added to help in the breakdown of larger food molecules into smaller molecules

Type of digestion --- Tube contents Carbohydrate Digestion TEST THAT WILL PERFORM IN LAB TO TEST FOR THE PRESENCE OF SMALLER MOLECULES IN THE PROCESS OF DIGESTION Type of digestion --- Tube contents Protein Digestion Lipid Digestion Carbohydrate Digestion Substrate (Reactant) Albumin Olive oil Starch Enzyme Trypsin Pancreatic lipase Amylase Expected product Amino acids Glycerol and fatty acids Maltose Assay for substrate Biuret test + violet +/ - pink pH test + decrease in pH -No pH change Iodine test + blue/ black -Brown/ yellow Assay for product Ninhydrin test + blue/ violet -colorless   Benedict’s test + red/ orange +/ - yellow/ green -blue