Improved Real-Time Multiplex Polymerase Chain Reaction Detection of Methylenetetrahydrofolate Reductase (MTHFR) 677C>T and 1298A>C Polymorphisms Using.

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Improved Real-Time Multiplex Polymerase Chain Reaction Detection of Methylenetetrahydrofolate Reductase (MTHFR) 677C>T and 1298A>C Polymorphisms Using Nearest Neighbor Model-Based Probe Design Raghunath P. Agarwal, Stephen M. Peters, Manijeh Shemirani, Nicolas von Ahsen The Journal of Molecular Diagnostics Volume 9, Issue 3, Pages 345-350 (July 2007) DOI: 10.2353/jmoldx.2007.060035 Copyright © 2007 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 1 Probe hybridization schemes. A: MTHFR 1298A>C detection (this study) using the probe of the mutant sense strand sequence. The position of the rare MTHFR 1317T>C mutation is also shown. B (boxed): All four possibilities to detect the MTHFR 1298A>C polymorphism with hybridization probes are presented. The resulting mismatch and ΔTm for the top ranking probe of each kind chosen by the MeltCalc software are also given. C: MTHFR 1298A>C detection18 using a probe of the wild-type sense strand sequence. The rare MTHFR 1317T>C is downstream of the probe position. The Journal of Molecular Diagnostics 2007 9, 345-350DOI: (10.2353/jmoldx.2007.060035) Copyright © 2007 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 2 A: Melting-curve analysis for the determination of 677C>T polymorphism; primers and probes used: 677F, 677R, 677-HP1, and 677-HP2. B: Melting-curve analysis for the determination of 1298A>C polymorphism; primers and probes used: 1298F, 1298R, 1298-HP1, and 1298-HP2. C: Melting-curve analysis for the determination of 1298A>C polymorphism; primers and probes used: 1298F, 1298R, FLU1298, and LC1298. The Journal of Molecular Diagnostics 2007 9, 345-350DOI: (10.2353/jmoldx.2007.060035) Copyright © 2007 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions