Omar Araim, MD, James Ballantyne, Andrew L. Waterhouse, PhD, Bauer E

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Presentation transcript:

Inhibition of vascular smooth muscle cell proliferation with red wine and red wine polyphenols Omar Araim, MD, James Ballantyne, Andrew L. Waterhouse, PhD, Bauer E. Sumpio, MD, PhD Journal of Vascular Surgery Volume 35, Issue 6, Pages 1226-1232 (June 2002) DOI: 10.1067/mva.2002.124358 Copyright © 2002 Sociey for Vascular Surgery and The American Association for Vascular Surgery Terms and Conditions

Fig. 1 Twelve-hour 3H-thymidine DNA synthesis assay for red wine. x axis represents bovine aortic SMC samples treated with different concentrations of dealcoholized red wine, shown as volume percentage. Negative control is 10% fetal bovine serum growth media. y axis represents 3H-thymidine incorporation measured as counts per minute (cpm). A, Results for typical experiment. B, Graph shows mean results for three experiments, normalized as percentage of control. *P <.05 compared with negative control. Journal of Vascular Surgery 2002 35, 1226-1232DOI: (10.1067/mva.2002.124358) Copyright © 2002 Sociey for Vascular Surgery and The American Association for Vascular Surgery Terms and Conditions

Fig. 2 Twelve-hour 3H-thymidine DNA synthesis assay for red wine polyphenol extract. x axis represents bovine aortic SMC samples treated with different concentrations of red wine polyphenol extract, shown as volume percentage. Negative control is 10% fetal bovine serum growth media. y axis represents 3H-thymidine incorporation measured as counts per minute (cpm). A, Results for typical experiment. B, Graph shows mean results for three experiments, normalized as percentage of control. *P <.05 compared with negative control. Journal of Vascular Surgery 2002 35, 1226-1232DOI: (10.1067/mva.2002.124358) Copyright © 2002 Sociey for Vascular Surgery and The American Association for Vascular Surgery Terms and Conditions

Fig. 3 Twelve-hour 3H-thymidine DNA synthesis assay for resveratrol. x axis represents bovine aortic SMC samples treated with different concentrations of resveratrol, in micromolar. Negative control is 10% fetal bovine serum growth media. y axis represents 3H-thymidine incorporation measured as counts per minute (cpm). A, Results for typical experiment. B, Graph shows mean results for three experiments, normalized as percentage of control. *P <.05 compared with negative control. Journal of Vascular Surgery 2002 35, 1226-1232DOI: (10.1067/mva.2002.124358) Copyright © 2002 Sociey for Vascular Surgery and The American Association for Vascular Surgery Terms and Conditions

Fig. 4 Gross morphologic examination of SMCs treated with dealcoholized red wine, red wine polyphenol extract, or resveratrol for 48 hours. Examination with low-power magnification (4×) shows no gross morphologic changes in treated SMC samples. However, dose-dependent decrease in cell number at higher concentrations is seen. Journal of Vascular Surgery 2002 35, 1226-1232DOI: (10.1067/mva.2002.124358) Copyright © 2002 Sociey for Vascular Surgery and The American Association for Vascular Surgery Terms and Conditions

Fig. 5 Trypan blue exclusion staining of SMCs treated with red wine, red wine polyphenol extract, and resveratrol for 12 hours. Trypan blue staining was performed to assess cytotoxicity of dealcoholized red wine, polyphenol extract, and resveratrol. Cytotoxic substances cause cell membrane damage, which allows cells to absorb trypan blue stain and thus appear blue. Examination with high-power magnification (400×) shows occasional blue-stained cells in all samples with no significant difference between treated and control groups. Journal of Vascular Surgery 2002 35, 1226-1232DOI: (10.1067/mva.2002.124358) Copyright © 2002 Sociey for Vascular Surgery and The American Association for Vascular Surgery Terms and Conditions