Airway smooth muscle remodeling is a dynamic process in severe long-standing asthma Muhannad Hassan, MD, Taisuke Jo, MD, PhD, Paul-André Risse, PhD,

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Presentation transcript:

Airway smooth muscle remodeling is a dynamic process in severe long-standing asthma Muhannad Hassan, MD, Taisuke Jo, MD, PhD, Paul-André Risse, PhD, Barbara Tolloczko, PhD, Catherine Lemière, MD, MSc, Ronald Olivenstein, MD, Qutayba Hamid, MD, PhD, James G. Martin, MD, DSc Journal of Allergy and Clinical Immunology Volume 125, Issue 5, Pages 1037-1045.e3 (May 2010) DOI: 10.1016/j.jaci.2010.02.031 Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 1 Correlation between PCNA and Ki 67 in airway tissues. PCNA+ or Ki 67+ nuclei were immunolocalized to α-SMA+ cells by using consecutive sections of bronchial biopsies. An illustrative example is shown (A and B). PCNA and Ki 67 proliferation indices were correlated (C). Data are mean cell percentages ± SE (n = 4 in severe, 3 in moderate, and 2 in control subjects). Scale bar = 50 μm. Journal of Allergy and Clinical Immunology 2010 125, 1037-1045.e3DOI: (10.1016/j.jaci.2010.02.031) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 2 HB-EGF and ASM cell proliferation. Human ASM cells were incubated with 0.1% FBS or 0.1% FBS treated with 10 ng/mL recombinant human HB-EGF for 4 or 24 hours. PCNA or Ki 67 proliferation index was then calculated by immunostaining using cytospins of human ASM cells (A). Human ASM cells were cultured in 10% FBS or 0.1% FBS for 3 or 6 hours, and HB-EGF mRNA expression was examined (B). Bars show mean ± SE. Journal of Allergy and Clinical Immunology 2010 125, 1037-1045.e3DOI: (10.1016/j.jaci.2010.02.031) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 3 Measurement of ASM mass and quantification of proliferating ASM cells in vivo. ASM mass and hyperplasia were examined by co-localization of PCNA immunoreactivity with α-SMA. An illustrative example is shown (A and B). ASM mass was expressed as the area of ASM normalized to total area of the biopsy specimen (C), and PCNA proliferation index was calculated (D). Bars show mean ± SE (n = 6 in moderate, 9 in severe and control subjects). Scale bar = 50 μm. Journal of Allergy and Clinical Immunology 2010 125, 1037-1045.e3DOI: (10.1016/j.jaci.2010.02.031) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 4 HB-EGF mRNA expression in bronchial biopsies by ISH. HB-EGF mRNA was detected by ISH in inflammatory cells, epithelial cells, and ASM bundles and demonstrated by pseudocolour images from green to red corresponding to intensity of the signal as shown in dark field images (B, E, H). The corresponding bright field images are shown in panels A, D and G. The HB-EGF density of expression was quantified in ASM (C, F) and epithelium (I). Bars show mean ± SE (n = 7 in moderate, 5 in severe and control subjects). Scale bar = 50 μm. Journal of Allergy and Clinical Immunology 2010 125, 1037-1045.e3DOI: (10.1016/j.jaci.2010.02.031) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 5 Immunohistochemical staining of HB-EGF in human airways. HB-EGF immunoreactivity was detected by immunohistochemical stains (red staining) in inflammatory cells, epithelial cells, and ASM bundles as shown in bright field (A, D, G) and their corresponding immunofluorescent images (B, E, H) and 3-dimensional (3D) images, which are shown according to thermo map (C, F, I). Scale bar = 50 μm. Journal of Allergy and Clinical Immunology 2010 125, 1037-1045.e3DOI: (10.1016/j.jaci.2010.02.031) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 6 Quantification of HB-EGF protein in airway tissues. Total HB-EGF protein expression in ASM was significantly increased in patients with severe asthma (P = .02 vs moderate; P = .006 vs control; A). There was also a significant increase in the HB-EGF density of expression within ASM in severe asthma compared with control subjects (P = .01, B). Epithelial HB-EGF protein immunoreactivity was comparable among all groups (C). Bars show mean ± SE (n = 8/group). Journal of Allergy and Clinical Immunology 2010 125, 1037-1045.e3DOI: (10.1016/j.jaci.2010.02.031) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

PCNA and Ki 67 correlation in vitro PCNA and Ki 67 correlation in vitro. Human ASM cells were incubated with 10% FBS, 0.1% FBS, or 0.1% FBS treated with 10 ng/mL recombinant HB-EGF for 4 or 24 hours. Cytospins were then performed and immunostained for PCNA or Ki 67 (A and B). Arrows indicate some of the PCNA+ cells. The proliferation indices of the 2 markers were then correlated (C). Data are mean cell percentages ± SE. Scale bar = 50 μm. Journal of Allergy and Clinical Immunology 2010 125, 1037-1045.e3DOI: (10.1016/j.jaci.2010.02.031) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions