Diagnosis of Aerococcus urinae infections: Importance of matrix-assisted laser desorption ionization time-of-flight mass spectrometry and broad-range 16S rDNA PCR  O. Opota, G. Prod'hom, C. Andreutti-Zaugg, M. Dessauges, L. Merz, G. Greub, J.-P. Chave, K. Jaton  Clinical Microbiology and Infection  Volume 22, Issue 1, Pages e1-e2 (January 2016) DOI: 10.1016/j.cmi.2015.08.026 Copyright © 2015 European Society of Clinical Microbiology and Infectious Diseases Terms and Conditions

Fig. 1 Importance of matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) and 16S rDNA PCR in Aerococcus urinae identification. The graph represents the identification of A. urinae from 1995 to 2015. From 1995 to 2004, no A. urinae strain was identified to the species level from clinical samples in our tertiary university hospital. After the introduction of MALDI-TOF MS into routine practice in our laboratory in 2009 May, we observed a significant increase in the rate of A. urinae identification. The broad-range PCR targeting the 16S rDNA gene became available in our laboratory in 2003. A. urinae was mainly identified from urine samples from patient suffering from urinary tract infection. Eleven severe cases of infection due to this organism are reported in this diagram (each mark represents one case); five of them were found with PCR targeting the 16S rRNA gene. The stars (*) mark 16S rDNA PCR performed directly on the clinical sample. Note that only two strains were resistant to ceftriaxone out of 176 strains for which an antibiotic susceptibility test had been performed. Clinical Microbiology and Infection 2016 22, e1-e2DOI: (10.1016/j.cmi.2015.08.026) Copyright © 2015 European Society of Clinical Microbiology and Infectious Diseases Terms and Conditions