Nuclear Receptor NR1H3 in Familial Multiple Sclerosis

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Nuclear Receptor NR1H3 in Familial Multiple Sclerosis Zhe Wang, A. Dessa Sadovnick, Anthony L. Traboulsee, Jay P. Ross, Cecily Q. Bernales, Mary Encarnacion, Irene M. Yee, Madonna de Lemos, Talitha Greenwood, Joshua D. Lee, Galen Wright, Colin J. Ross, Si Zhang, Weihong Song, Carles Vilariño-Güell Neuron Volume 90, Issue 5, Pages 948-954 (June 2016) DOI: 10.1016/j.neuron.2016.04.039 Copyright © 2016 Elsevier Inc. Terms and Conditions

Figure 1 Simplified Pedigree for Families Presenting the NR1H3 p.Arg415Gln Mutation Males are represented by squares and females by circles; the proband is arrowed and a diagonal line indicates deceased subjects. Patients diagnosed with MS have black-filled symbols, and mutation carriers of unknown phenotype have gray-filled symbols. Both families are of Caucasian descent. Heterozygote mutation carriers (M) with corresponding age at onset of disease and wild-type (WT) genotypes are indicated. An asterisk indicates an inferred mutation carrier. NA, not available. Neuron 2016 90, 948-954DOI: (10.1016/j.neuron.2016.04.039) Copyright © 2016 Elsevier Inc. Terms and Conditions

Figure 2 NR1H3 p.Arg415Gln Conservation in Orthologs and Human Paralogs Protein homologs were aligned via ClustalO. Amino acid position for NR1H3 p.Arg415Gln is highlighted in black. Protein homologs with amino acid positions differing from those of the human NR1H3 sequence are indicated in gray. RefSeq accession numbers are provided for orthologs; and gene, protein, and RefSeq accession numbers for paralogs. An arrow indicates the exclusion of two or three amino acids from Caenorhabditis elegans at these positions. Neuron 2016 90, 948-954DOI: (10.1016/j.neuron.2016.04.039) Copyright © 2016 Elsevier Inc. Terms and Conditions

Figure 3 Structural and Functional Analysis of NR1H3 (A) Crystal structure of NR1H3 (LXRA) and NR2B1 (RXRA) heterodimer showing the highly conserved arginine residues in their corresponding LBD was generated with PyMol (PDB: 2ACL). Inserts provide a detailed view of the LXRA wild-type (Arg) and mutated (Gln) protein structure. (B) FLAG-LXRA wild-type (WT) and p.Arg415Gln mutant (R415Q) were co-expressed with RXRA-mycHis (+) or empty vector pcDNA4-myc-his-A (V) in HEK cells with or without 10 μM LXRA agonist T0901317 overnight. RXRA was pulled down from the lysates with nickel-charged magnetic beads. Cell lysates and precipitates were immunoblotted with FLAG or Myc antibodies. (C) Transcriptional activation of control (pGL-pl) and LXR response element (LXRE)-containing plasmids after co-transfection with empty vector, wild-type (WT), or mutant LXRA (R415Q). Firefly/Renilla luciferase activity (mean ± SE) for the different constructs, with and without the presence of 10 μM of an LXRA agonist (T0901317) normalized to pGL-pl under the same conditions are provided. Statistically significant Fisher’s least significant difference (LSD) post hoc values are provided. r.u., relative units. Neuron 2016 90, 948-954DOI: (10.1016/j.neuron.2016.04.039) Copyright © 2016 Elsevier Inc. Terms and Conditions