Loss of vimentin in B cells impairs antibody response in vivo.

Slides:

Advertisements

Similar presentations

Shigella-specific serum IgG and IgA endpoint titers in guinea pigs immunized intranasally with S. flexneri 2a InvaplexNAT or InvaplexAR. Shigella-specific.

Advertisements

Cheng-Ming Sun, Edith Deriaud, Claude Leclerc, Richard Lo-Man Immunity

Melanoma donor (MD) NK cells are functionally impaired/exhausted.

In vivo retroviral gene transfer by direct intrafemoral injection results in correction of the SCID phenotype in Jak3 knock-out animals by Christine S.

by Silke Huber, Reinhard Hoffmann, Femke Muskens, and David Voehringer

Malaria-specific transgenic CD4+ T cells protect immunodeficient mice from lethal infection and demonstrate requirement for a protective threshold of antibody.

by Norman Nausch, Ioanna E

Macrophages from C3-deficient mice have impaired potency to stimulate alloreactive T cells by Wuding Zhou, Hetal Patel, Ke Li, Qi Peng, Marie-Bernadette.

Volume 13, Issue 5, Pages (November 2000)

Culture expanded primary chondrocytes have potent immunomodulatory properties and do not induce an allogeneic immune response P. Lohan, O. Treacy, K.

Volume 30, Issue 4, Pages (April 2009)

Expansion of FOXP3high regulatory T cells by human dendritic cells (DCs) in vitro and after injection of cytokine-matured DCs in myeloma patients by Devi.

Increased survival is a selective feature of human circulating antigen-induced plasma cells synthesizing high-affinity antibodies by Inés González-García,

by Éric Aubin, Réal Lemieux, and Renée Bazin

Preactivation with IL-12, IL-15, and IL-18 Induces CD25 and a Functional High-Affinity IL-2 Receptor on Human Cytokine-Induced Memory-like Natural Killer.

Cognate interaction with antigen-specific T cells is required for the response of IgG-type memory B cells. Cognate interaction with antigen-specific T.

by Sheng F. Cai, Xuefang Cao, Anjum Hassan, Todd A

TACI deficiency impairs sustained Blimp-1 expression in B cells decreasing long-lived plasma cells in the bone marrow by Shoichiro Tsuji, Catarina Cortesão,

Volume 11, Issue 12, Pages (June 2015)

IL-21 inhibits T cell IL-2 production and impairs Treg homeostasis

Volume 23, Issue 11, Pages (June 2018)

B-cell differentiation associates with down-regulation of vimentin expression. B-cell differentiation associates with down-regulation of vimentin expression.

Volume 20, Issue 3, Pages (July 2017)

Volume 30, Issue 3, Pages (March 2009)

Volume 18, Issue 5, Pages (May 2003)

Maturation, Ag presentation capacity, and IL-12 production of DCs

CD22 is a negative regulator of B-cell receptor signalling

CXCR5+/+ TFH cells are dispensable for maintenance of the LCMV-specific antibody response. CXCR5+/+ TFH cells are dispensable for maintenance of the LCMV-specific.

Humoral Immunity Due to Long-Lived Plasma Cells

CD4 T cells are responsible for the intensified joint pathology at 6 d postinfection. CD4 T cells are responsible for the intensified joint pathology at.

by Kalpana Parvathaneni, and David W. Scott

Regulatory T Cells Control Antigen-Specific Expansion of Tfh Cell Number and Humoral Immune Responses via the Coreceptor CTLA-4 James Badger Wing, Wataru.

Volume 27, Issue 3, Pages (September 2007)

DC-derived EV differentiate monocytes.

Volume 7, Issue 1, Pages (April 2014)

Volume 17, Issue 1, Pages (September 2016)

Fluorescent In Vivo Detection Reveals that IgE+ B Cells Are Restrained by an Intrinsic Cell Fate Predisposition Zhiyong Yang, Brandon M. Sullivan, Christopher D.C.

Volume 44, Issue 4, Pages (April 2016)

Vimentin regulates intracellular trafficking in B cells.

Volume 37, Issue 3, Pages (September 2012)

Monte M. Winslow, Elena M. Gallo, Joel R. Neilson, Gerald R. Crabtree

Matthew Charles Woodruff, Eui Ho Kim, Wei Luo, Bali Pulendran

IgH Class Switch Recombination to IgG1 in DNA-PKcs-Deficient B Cells

T Cell–Mediated Elimination of B7.2 Transgenic B Cells

Adaptive immune responses and NK activity.

Vimentin is dispensable for multiple B cell–intrinsic processes.

by Tadayuki Yago, Nan Zhang, Liang Zhao, Charles S

In Vivo Expansion of Regulatory T cells With IL-2/IL-2 mAb Complexes Prevents Anti- factor VIII Immune Responses in Hemophilia A Mice Treated With Factor.

Fig. 5 Treatment with an OX40 agonist antibody of 3-week-old HBVtgRag−/− mice or mice with chronic HBV disease results in an altered immune response to.

Vimentin reorganisation requires actin and microtubules.

IL-6 neutralization decreases T cell proliferation and cytokine production upon restimulation by allogeneic HUVEC. CIITA transduced HUVECs were cocultured.

The 3′F allele does not recapitulate the systemic hyperimmune phenotypes associated with mir-146a deficiency. The 3′F allele does not recapitulate the.

Toll-Dependent Control Mechanisms of CD4 T Cell Activation

Effect of dose level on the anti-keyhole limpet haemocyanin (KLH) IgG response in the multiple-ascending dose (MAD) study. Effect of dose level on the.

Volume 25, Issue 12, Pages e5 (December 2018)

Removal of both B-1–mediated anti-PC natural IgM and B-2–mediated anti-PEG IgM responses abrogates ABC in mice. Removal of both B-1–mediated anti-PC natural.

Volume 6, Issue 4, Pages (February 2014)

Increased anti-DNA Abs in 2KO-Bcl6TC mice.

Volume 28, Issue 5, Pages (May 2008)

Local Inhibition of Complement Improves Mesenchymal Stem Cell Viability and Function After Administration Yan Li, John Fung, Feng Lin Molecular Therapy

Volume 31, Issue 5, Pages (November 2009)

Fig. 2 Phenotypic analyses of Bcl11b-deficient Treg cells.

Engineered binding to erythrocytes induces immunological tolerance to E. coli asparaginase by Kristen M. Lorentz, Stephan Kontos, Giacomo Diaceri, Hugues.

Volume 13, Issue 11, Pages (December 2015)

The CD8+ cytotoxic T-cell response in Ron TK−/− hosts in response to tumors is necessary and sufficient to block metastasis. The CD8+ cytotoxic T-cell.

p110δ activity is important for autoantibody responses.

Hypoxic Ag-specific CD8+ T cells are less functional and less proliferative. Hypoxic Ag-specific CD8+ T cells are less functional and less proliferative.

IL35 regulation of tumor growth is accompanied by suppression of CD4+ effector T-cell activity and expansion of Tregs. IL35 regulation of tumor growth.

Volume 10, Issue 5, Pages (February 2015)

Regulation of antibacterial B cell responses in the peritoneal cavity via MyD88 signaling in FRCs. Regulation of antibacterial B cell responses in the.

Presentation transcript:

Loss of vimentin in B cells impairs antibody response in vivo. Loss of vimentin in B cells impairs antibody response in vivo. (A) Representative histograms showing CTV dilution profiles of primary B cells cultured in vitro. Primary B cells from WT and Vim−/− mice were purified and cultured with anti-IgM and IL-4 in vitro for 4 d. The CTV dilution profile was monitored at different time points using flow cytometry to assess cell proliferation. (B) Representative histograms showing the CTV dilution profile of primary B cells cultured in vitro. Primary B cells from WT and Vim−/− mice were cultured for 4 d in the presence of LPS and IL-4 (left panel), or anti-IgM, CD40L, and IL-4 (right panel) in vitro. The CTV dilution was assessed on day 4 using flow cytometry. (C, D) Representative flow cytometry plots and the corresponding quantification showing B-cell differentiation into (left panel) plasma cells (CD138+IgD−) or (right panel) those that underwent class switch recombination (IgG1+IgD−) of B cells cultured in vitro for 4 d in the presence of (C) LPS and IL-4 or (D) anti-IgM, CD40L, and IL-4. (E) WT and Vim−/− mice were immunised with 2 μg NP-LPS via intraperitoneal injection and serum NP-specific IgM titre on days 3, 6, and 9 after immunisation was measured using ELISA. Two-way ANOVA was used to determine statistical significance. (F) WT and Vim−/− mixed bone marrow chimeras were immunised with 50 μg of NP conjugated to keyhole limpet haemocyanin with alum via intraperitoneal injections. Blood samples were collected on days –1, 3, 7, 14, 21, 28, and 69 after immunisation and serum antigen-specific IgG was measured using ELISA. Graphs depict the titres of anti-NP23 (left panel) and anti-NP3 (right panel) IgG. Two-way ANOVA was used to determine statistical significance. (G) The ratios of anti-NP23 and anti-NP3 IgG at each time point were calculated to determine the extent of affinity maturation of the IgG. Two-way ANOVA was used to determine statistical significance. Carlson Tsui et al. LSA 2018;1:e201800060 © 2018 Tsui et al.