Photodynamic therapy inactivates extracellular matrix–basic fibroblast growth factor: Insights to its effect on the vascular wall Glenn M. LaMuraglia,

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Presentation transcript:

Photodynamic therapy inactivates extracellular matrix–basic fibroblast growth factor: Insights to its effect on the vascular wall Glenn M. LaMuraglia, MD, Farzin Adili, MD, Seth J. Karp, MD, Randolph G. Statius van Eps, MD, Michael T. Watkins, MD Journal of Vascular Surgery Volume 26, Issue 2, Pages 294-301 (August 1997) DOI: 10.1016/S0741-5214(97)70192-7 Copyright © 1997 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North America Chapter Terms and Conditions

Fig. 1 PDT inactivation of soluble bFGF (1 ng/ml). Percent of bFGF remaining after inactivation by PDT is plotted versus fluence administered. Values are mean ± SEM of three experiments in triplicate. *p < 0.001 versus initial concentration. ‡p < 0.02 versus 5 J/cm2. Journal of Vascular Surgery 1997 26, 294-301DOI: (10.1016/S0741-5214(97)70192-7) Copyright © 1997 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North America Chapter Terms and Conditions

Fig. 2 PDT inactivation of ECM-bound bFGF. Concentration of bFGF (pg/ml) extracted from matrix (9.6 cm2 plate) is plotted for PDT-treated and control matrices: matrix (untreated), CASPc (drug-only), or light (laser-only). Values are mean ± SEM of two experiments in triplicate. *p < 0.0001 versus matrix. Journal of Vascular Surgery 1997 26, 294-301DOI: (10.1016/S0741-5214(97)70192-7) Copyright © 1997 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North America Chapter Terms and Conditions

Fig. 3 PDT inhibition of ECM proliferative stimulus on SMCs. Proliferative stimulus of ECM on SMCs, assessed by 3H-thymidine incorporation, is plotted for PDT-treated and control matrices: matrix (untreated), CASPc (drug-only), or light (laser-only). To determine whether proliferative stimulus of ECM could be restored after PDT, bFGF (500 pg) was incubated with untreated and PDT matrix before addition of SMCs (+bFGF). Values are mean ± SEM of three experiments in triplicate. *p < 0.0001 versus matrix. Journal of Vascular Surgery 1997 26, 294-301DOI: (10.1016/S0741-5214(97)70192-7) Copyright © 1997 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North America Chapter Terms and Conditions

Fig. 4 Immunohistochemical micrographs (original magnification, 200×) of rat common carotid arteries depict bFGF by immunoperoxidase staining and counterstained with nuclear-fast red. A, Control artery with intimal and medial staining of bFGF. B, PDT artery immediately after in vivo treatment with no evidence of staining for bFGF. Bar represents 50 mm. Journal of Vascular Surgery 1997 26, 294-301DOI: (10.1016/S0741-5214(97)70192-7) Copyright © 1997 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North America Chapter Terms and Conditions

Fig. 4 Immunohistochemical micrographs (original magnification, 200×) of rat common carotid arteries depict bFGF by immunoperoxidase staining and counterstained with nuclear-fast red. A, Control artery with intimal and medial staining of bFGF. B, PDT artery immediately after in vivo treatment with no evidence of staining for bFGF. Bar represents 50 mm. Journal of Vascular Surgery 1997 26, 294-301DOI: (10.1016/S0741-5214(97)70192-7) Copyright © 1997 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North America Chapter Terms and Conditions