Computer-assisted motility analysis of spermatozoa obtained after spermatogonial stem cell transplantation in the mouse Ellen Goossens, Ph.D., Gert De.

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Computer-assisted motility analysis of spermatozoa obtained after spermatogonial stem cell transplantation in the mouse Ellen Goossens, Ph.D., Gert De Block, Herman Tournaye, Ph.D., M.D. Fertility and Sterility Volume 90, Issue 4, Pages 1411-1416 (October 2008) DOI: 10.1016/j.fertnstert.2007.08.035 Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions

Figure 1 Vitality, motility, and progressive motility of spermatozoa obtained from fertile control male mice (Ctrl) and mice that had undergone spermatogonial stem cell transplantation (WW). Spermatozoa were cultured for 24 hours. Significance data for treatment effect by repeated measures analysis of variance were as follows: for vitality, effect of treatment was P=.336, effect of time was P<.001, and combined effect was P=.899; for motility, effect of treatment was P=.012, effect of time was P<.001, and combined effect was P=.003; and for progressive motility, effect of treatment was P=.012, effect of time was P<.001, and combined effect was P=.003. Fertility and Sterility 2008 90, 1411-1416DOI: (10.1016/j.fertnstert.2007.08.035) Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions

Figure 2 The effect of the concentration of the sperm sample on its motility. The Spearman correlation coefficient for not-normally divided data was 0.02. WW = mice that had undergone spermatogonial stem cell transplantation. Fertility and Sterility 2008 90, 1411-1416DOI: (10.1016/j.fertnstert.2007.08.035) Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions

Figure 3 Individual sperm movement characteristics of spermatozoa obtained from fertile control male mice (ctrl) and mice that had undergone spermatogonial stem cell transplantation (WW). Spermatozoa were cultured for 24 hours. Significance data for treatment effect by repeated measures analysis of variance were as follows: for VAP, effect of treatment was P=.017, effect of time was P<.001, and combined effect was P=.032; for VSL, effect of treatment was P=.017, effect of time was P<.001, and combined effect was P=.026; for VCL, effect of treatment was P=.030, effect of time was P<.001, and combined effect was P=.118; for ALH, effect of treatment was P=.006, effect of time was P<.001, and combined effect was P=.070; for BCF, effect of treatment was P=.091, effect of time was P<.001, and combined effect was P=.293; for STR, effect of treatment was P=.049, effect of time was P<.001, and combined effect was P=.023; for LIN, effect of treatment was P=.062, effect of time was P<.001, and combined effect was P=.013; and for hyperactivity, effect of treatment was P=.029, effect of time was P<.001, and combined effect was P=.255. Fertility and Sterility 2008 90, 1411-1416DOI: (10.1016/j.fertnstert.2007.08.035) Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions