Ex vivo characterization of articular cartilage and bone lesions in a rabbit ACL transection model of osteoarthritis using MRI and micro-CT Danika L.

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Ex vivo characterization of articular cartilage and bone lesions in a rabbit ACL transection model of osteoarthritis using MRI and micro-CT Danika L.

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Ex vivo characterization of articular cartilage and bone lesions in a rabbit ACL transection model of osteoarthritis using MRI and micro-CT Danika L. Batiste, M.Sc., Alexandra Kirkley, M.D., F.R.C.S.(C), Sheila Laverty, M.V.B., Dipl. A.C.V.S., E.C.V.S., Lisa M.F. Thain, M.D., F.R.C.P.(C), Alison R. Spouge, M.D., F.R.C.P.(C), David W. Holdsworth, Ph.D. Osteoarthritis and Cartilage Volume 12, Issue 12, Pages 986-996 (December 2004) DOI: 10.1016/j.joca.2004.08.010 Copyright © 2004 OsteoArthritis Research Society International Terms and Conditions

Fig. 1 Coronal micro-CT slices of a control (a) and ACLT joint demonstrating osteophyte development (b). Note the appearance of normal bone contour visible in (a), which is also visible underlying the newly formed, reduced density osteophytic bone indicated by arrows in (b). Osteoarthritis and Cartilage 2004 12, 986-996DOI: (10.1016/j.joca.2004.08.010) Copyright © 2004 OsteoArthritis Research Society International Terms and Conditions

Fig. 2 Macroscopic photos of full-thickness ulceration on the femur (a) and tibia (b). Ulceration indicated by arrows. Osteoarthritis and Cartilage 2004 12, 986-996DOI: (10.1016/j.joca.2004.08.010) Copyright © 2004 OsteoArthritis Research Society International Terms and Conditions

Fig. 3 Left column (A, C, E, G): macroscopic assessment of femorotibial cartilage grade in control, 4-, 8-, and 12-week ACLT specimens. ANOVA values presented as mean±s.e.m. for all four compartments (LFC, MFC, LTP, and MTP). Control 4-week was not statistically different from Control 12-week. Control vs 4-week ACLT *P<0.01, Control vs 8-week ACLT †P<0.01, Control vs 8-week ACLT ††P<0.001, Control vs 12-week ACLT ‡P<0.01, Control vs 12-week ACLT ‡‡P<0.001, 4-week vs 8-week ACLT #P<0.05, 4-week vs 12-week ACLT ##P<0.01. Right column (B, D, F, H): MRI evaluation of femorotibial cartilage grade in control, 4-, 8-, and 12-week ACLT specimens. ANOVA values presented as mean±s.e.m. for all four compartments (LFC, MFC, LTP, and MTP). Control 4-week was not statistically different from Control 12-week. Control vs 12-week ACLT *P<0.05, Control vs 8-week ACLT ‡P<0.01, 4-week ACLT vs 12-week ACLT †P<0.05. (Please note that control animals were not studied at the 8-week time-point.) Osteoarthritis and Cartilage 2004 12, 986-996DOI: (10.1016/j.joca.2004.08.010) Copyright © 2004 OsteoArthritis Research Society International Terms and Conditions

Fig. 4 Sagittal MRI slices (a–d) at various time-points depicting progressive cartilage lesions from 4 to 12 weeks post-ACLT. Rectangular inset (e–h) magnified to clearly illustrate femoral lesions (arrows indicate extent of lesion). Osteoarthritis and Cartilage 2004 12, 986-996DOI: (10.1016/j.joca.2004.08.010) Copyright © 2004 OsteoArthritis Research Society International Terms and Conditions

Fig. 5 Scatterplot of bone volume fraction (BV/TV) as a function of volumetric bone mineral density (vBMD). Osteoarthritis and Cartilage 2004 12, 986-996DOI: (10.1016/j.joca.2004.08.010) Copyright © 2004 OsteoArthritis Research Society International Terms and Conditions

Fig. 6 Micro-CT analysis of vBMD at various time-points. ANOVA values presented as mean±s.e.m. for LFC, MFC, LTP, and MTP. Control vs 4-week ACLT †P<0.001, Control vs 8-week ACLT ††P<0.001, 4-week vs 8-week ACLT *P<0.05, 4-week vs 8-week ACLT **P<0.01, 4-week vs 12-week ACLT #P<0.05, 4-week vs 12-week ACLT ##P<0.001, 8-week vs 12-week ACLT ‡P<0.001. Osteoarthritis and Cartilage 2004 12, 986-996DOI: (10.1016/j.joca.2004.08.010) Copyright © 2004 OsteoArthritis Research Society International Terms and Conditions

Fig. 7 Coronal micro-CT slices (a–d) at various time-points depicting osteophyte development. Volume rendered micro-CT (e–h) clearly illustrates osteophyte volume (red) and irregular bone surface contour. Osteoarthritis and Cartilage 2004 12, 986-996DOI: (10.1016/j.joca.2004.08.010) Copyright © 2004 OsteoArthritis Research Society International Terms and Conditions

Fig. 8 Micro-CT analysis of osteophyte volume at various time-points. ANOVA values presented as mean±s.e.m. for combined femur and tibia (*P<0.05). Osteoarthritis and Cartilage 2004 12, 986-996DOI: (10.1016/j.joca.2004.08.010) Copyright © 2004 OsteoArthritis Research Society International Terms and Conditions