Fig. 3 TLR8 signaling induces CXCL4 and IFN-α secretion by SSc PDCs.

Slides:

Advertisements

Similar presentations

Volume 94, Issue 2, Pages (March 2014)

Advertisements

Volume 108, Pages (January 2018)

Anifrolumab inhibits cytokine production, plasmacytoid dendritic cell (pDC) activation and the type I (interferon gene signature) IFN gene signature. Anifrolumab.

Fig. 1 CSF1 is increased in blood of melanoma patients and correlates with disease progression. CSF1 is increased in blood of melanoma patients and correlates.

IL-1β stimulates CXCL5 and CXCL8 gene expression and protein secretion in A549 cells in a time- and dose-dependent manner. IL-1β stimulates CXCL5 and CXCL8.

Fig. 4. PVSRIPO infection of DCs is sublethal, is marginally productive, and induces sustained proinflammatory cytokine production. PVSRIPO infection of.

Immune profiling of ZIKV-infected PBMCs

Fig. 1. IL-6 is associated with resistance to EGFR TKIs and is induced by stress hormones. IL-6 is associated with resistance to EGFR TKIs and is induced.

IL-10 disrupts the Brd4-docking sites to inhibit LPS-induced CXCL8 and TNF-α expression in monocytes: Implications for chronic obstructive pulmonary disease

IFNα, but not IFNλ, treatment induces cytokine secretion from human immune cells IFNα, but not IFNλ, treatment induces cytokine secretion from human immune.

Cytokine and chemokine expression of flucloxacillin-specific T cell clones (TCC) of patient P1. Cytokine and chemokine expression of flucloxacillin-specific.

Enhancement of the host immune responses in cutaneous T-cell lymphoma by CpG oligodeoxynucleotides and IL-15 by Maria Wysocka, Bernice M. Benoit, Sarah.

Fig. 2 TLR8 is aberrantly expressed on pDCs from SSc patients.

PVSRIPO-mediated APC activation occurs in immunosuppressive conditions

Role of monocytes in NK cell activity.

Fig. 1 pDCs infiltrate the skin of SSc patients and spontaneously secrete IFN-α and CXCL4. pDCs infiltrate the skin of SSc patients and spontaneously secrete.

Age-Related Differences in Sensitivity of Peripheral Blood Monocytes to Lipopolysaccharide and Staphylococcus Aureus Toxin B in Atopic Dermatitis Marie.

Fig. 7. The PD-L1 defect is evident in HSPCs from T1D patients.

Expression of CD36 and psap in a TMA of human ovarian cancer patients

Fig. 4 CXCL4 potentiates TLR8-mediated activation of SSc pDCs.

Fig. 5. Stimulation of EPO expression and secretion under hypoxic culture conditions. Stimulation of EPO expression and secretion under hypoxic culture.

Identification of bioactive compounds modulating STING activation

Fig. 3. Applying the rapid test to analyze human patient sera.

Figure 4 Abundance of cytokines which showed significant difference in expression in the plasma and the cultured PBMC of patients with RRMS Abundance of.

Volume 7, Issue 4, Pages (October 2016)

Fig. 4. MATE1 transcription in RCC.

Fig. 7 pDCs are critical for the maintenance of skin fibrosis and for the presence of CXCL4 in the skin. pDCs are critical for the maintenance of skin.

Human Type 2 Myeloid Dendritic Cells Produce Interferon-λ and Amplify Interferon-α in Response to Hepatitis C Virus Infection Shuye Zhang, Karen Kodys,

Fig. 2. Vascular inflammation blocks perivascular adipocyte differentiation through paracrine signals. Vascular inflammation blocks perivascular adipocyte.

Antiviral responses of human Fallopian tube epithelial cells to toll-like receptor 3 agonist poly(I:C) Mimi Ghosh, Ph.D., Todd M. Schaefer, Ph.D., John.

Fig. 8 TLR8 exacerbates disease in the BLM-induced fibrosis model.

Fig. 6 pDCs infiltrate the skin of BLM-treated mice, and their depletion attenuates skin fibrosis. pDCs infiltrate the skin of BLM-treated mice, and their.

IFN-β, IL-6, TNF-α and CCL2 release were determined by ELISA in culture supernatants of PBMC stimulated with LPS from Salmonella abortusequi (1 µg/mL Sigma–Aldrich.

Fig. 3. β-AR signaling induces IL-6 in NSCLC cells via activation of PKC and CREB. β-AR signaling induces IL-6 in NSCLC cells via activation of PKC and.

Fig. 5 CXCL4 potentiates TLR9-mediated activation but has minimal effect on TLR7-mediated activation of pDCs purified from SSc or HDs. CXCL4 potentiates.

Fig. 7. Inhibitory mechanisms of C12G6.

Fig. 2 Fas controls IL-1RA–sEV secretion in murine MSCs.

Volume 138, Issue 1, Pages (January 2010)

Volume 143, Issue 6, Pages e8 (December 2012)

Fig. 4 TNF-α up-regulates Fas/Fap-1 expression to promote IL-1RA–sEV release in murine MSCs. TNF-α up-regulates Fas/Fap-1 expression to promote IL-1RA–sEV.

Fig. 1 CpG induces the expression of OX40 on CD4 T cells.

Fig. 6. pKL cells revert hyperglycemia in NOD mice in vivo.

Defective negative regulation of Toll-like receptor signaling leads to excessive TNF-α in myeloproliferative neoplasm by Hew Yeng Lai, Stefan A. Brooks,

Bexarotene is neuroprotective in mouse and human HD neurons in vitro

IL-12 affects Dermatophagoides farinae–induced IL-4 production by T cells from pediatric patients with mite-sensitive asthma Takeshi Noma, MD, PhD, Izumi.

Fig. 5. Prophylactic and therapeutic efficacy of C12G6 in ferrets.

Fig. 5 DMN-Tre labeling is selective for live mycobacteria.

IL-4 and IL-13 Alter Plasmacytoid Dendritic Cell Responsiveness to CpG DNA and Herpes Simplex Virus-1 Jurjen Tel, Ruurd Torensma, Carl G. Figdor, I.

Fig. 6 DMF inhibits NF-κB translocation upon infection.

Fig. 5 Extended local release of R848 increases the number of innate and adaptive antitumor immune cells and cytokines. Extended local release of R848.

Figure 3 Cytokine gene expression in PBMC stimulated with PPD or MBP in vitroCytokine messenger RNA transcripts were isolated from peripheral blood mononuclear.

CSF1 secretion by melanoma cells is induced by CTL-derived cytokines

CpG-C ISS lose their ability to induce IFN-α from human PDCs while retaining the ability to induce maturation when encapsulated into pH 5.75 liposomes.

DC subset cooperation for activation of antiviral T cells.

Fig. 2 Role of TLR9 in macrophage activation.

David M. Essayan, MD, Charity C

PD-1 blockade enhances T-cell function.

Fig. 2 AcPGP induces IL-8 and G-CSF release from human bronchial epithelial cells. AcPGP induces IL-8 and G-CSF release from human bronchial epithelial.

Fig. 4. PVSRIPO infection of DCs is sublethal, is marginally productive, and induces sustained proinflammatory cytokine production. PVSRIPO infection of.

Figure 3 Fingolimod inhibits TNF-α secretion by human monocytes Peripheral blood mononuclear cells from healthy donors were briefly exposed to increasing.

KIF13A depletion leads to a drop in virus titers, without affecting viral protein expression. KIF13A depletion leadsto a drop in virus titers, without.

Fig. 2 IT1t prevents TLR7-mediated inflammation in pDCs.

Fig. 3 IT1t blocks TLR7-mediated IFN production of circulating pDCs in PBMC cultures. IT1t blocks TLR7-mediated IFN production of circulating pDCs in PBMC.

IL-27 induces expression of multiple IR by CD8+ T cells.

Fig. 6 IT1t controls induced and spontaneous inflammation in vitro in cells from patients with SLE. IT1t controls induced and spontaneous inflammation.

BMS blocks functional responses in primary immune cells driven by IFNα

pDCs from the melanoma environment responded to TLR-L stimulation.

Plasmacytoid dendritic cells promote systemic sclerosis with a key role for TLR8 by Marie Dominique Ah Kioon, Claudio Tripodo, David Fernandez, Kyriakos.

RT-TEX induce activation and IFN-I production by primary DCs

Fig. 6 Methionine oxidation catalyzed by viperin increases the stability and function of RNA helicase RIG-I. Methionine oxidation catalyzed by viperin.

Presentation transcript:

Fig. 3 TLR8 signaling induces CXCL4 and IFN-α secretion by SSc PDCs. TLR8 signaling induces CXCL4 and IFN-α secretion by SSc PDCs. (A) Gene expression levels of CXCL4 were quantified in total PBMC or pDC–depleted (dep) PBMC prepared from either HDs (n = 6) or SSc patients (n = 23) by qPCR. Statistical significance was evaluated using the unpaired Mann-Whitney test between HD and SSc PBMC and with a paired t test between PBMC and pDC-depleted PBMC from SSc patients. (B) Purified pDCs from HDs (n = 4 to 10) were cultured either with media (Med) alone as a control or in the presence of a TLR7 agonist [heat-inactivated VR95 influenza virus at a multiplicity of infection (MOI) of 2], a TLR8 agonist (ORN-8L at 200 μg/ml), or a TLR9 agonist (CpG-C274 at 0.5 μM), and 24 hours later, CXCL4 in the supernatants was measured by ELISA. Results were normalized to the value of media alone and represented as means ± SEM. (C and D) pDCs purified from SSc patients (n = 15 and 12, respectively) were cultured for 24 hours either in media alone or with either (C) ORN-8L or (D) CpG-C274, and CXCL4 production was analyzed by ELISA. Results were normalized to control and represented as means ± SEM. (E) HD (n = 10) and SSc pDCs (n = 20) were stimulated with ORN-8L for 24 hours, and supernatants were collected for measurement of IFN-α, IP-10, IL-6, and TNF by multiplex bead assay. Results were normalized to 10,000 cells. (F) Purified pDCs from SSc patients (n = 12) were cultured either with media alone or in the presence of a TLR9 agonist for 24 hours, and supernatants were collected for measurement of IFN-α, IP-10, IL-6, and TNF by multiplex bead assay. Results were normalized to 10,000 cells. All results are represented as means ± SEM. For (B) to (E), statistical significance was evaluated using a Mann-Whitney U test. *P < 0.05, **P < 0.01, ***P < 0.001. Marie Dominique Ah Kioon et al., Sci Transl Med 2018;10:eaam8458 Published by AAAS