The PI3K–Akt pathway is essential for the in vivo maintenance of murine Tregs. The PI3K–Akt pathway is essential for the in vivo maintenance of murine.

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The PI3K–Akt pathway is essential for the in vivo maintenance of murine Tregs. The PI3K–Akt pathway is essential for the in vivo maintenance of murine Tregs. Mice were conditioned with wortmannin (WM; 40 μg), triciribine (TCN; 50 μg), MK-2206 (10 μg), or DMSO for 1 week on alternate days before flow cytometric analysis of splenocytes. Alternatively, mice were conditioned on alternate days with either wortmannin or DMSO for a week before vaccination with E7. One week after E7 vaccination, splenocytes were harvested, and the E7 immune response was assayed by ELISPOT. A, mice treated with DMSO vehicle contained similar percentages of CD4 T cells compared with wortmannin-, triciribine-, or MK-2206–treated animals (P = 0.1). B, the number of FoxP3+ cells in CD4+ T cells was significantly reduced in the mice treated with the inhibitors compared with DMSO (P < 0.05). C, vaccination resulted in a significant increase in E7-reactive T cells. Wortmannin and triciribine treatment significantly increased the number of E7-reactive cells compared with DMSO-treated controls. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001. Rasha Abu-Eid et al. Cancer Immunol Res 2014;2:1080-1089 ©2014 by American Association for Cancer Research