Characterization of Dac g 4, a major basic allergen from Dactylis glomerata pollen Virginie Leduc-Brodard, PhDa, Filipe Inacio, MDa, Michel Jaquinod,

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Presentation transcript:

Characterization of Dac g 4, a major basic allergen from Dactylis glomerata pollen Virginie Leduc-Brodard, PhDa, Filipe Inacio, MDa, Michel Jaquinod, PhDb, Eric Forest, PhDb, Bernard David, MDa, Gabriel Peltre, PhDa Journal of Allergy and Clinical Immunology Volume 98, Issue 6, Pages 1065-1072 (December 1996) DOI: 10.1016/S0091-6749(96)80193-X Copyright © 1996 Mosby, Inc. Terms and Conditions

FIG. 1 BIAcore overlaid sensorgrams showing the binding of Dac g 4 on RAMFcγ-presented mAbs. After injection of mAbs (A to H, 50 μg/ml in HBS-P20), immobilization level of mAbs was considered as the baseline. a, Injection of Dac g 4 (10 μg/ml in HBS-P20); b, HBS-P20 injection; c, regeneration of the flow cell. RU, Resonance units. Journal of Allergy and Clinical Immunology 1996 98, 1065-1072DOI: (10.1016/S0091-6749(96)80193-X) Copyright © 1996 Mosby, Inc. Terms and Conditions

FIG. 2 SDS-PAGE of SEs from different grass, weed, and tree pollens (A) silver-stained, followed by immunoblotting with biotinylated mAb H (B). Lanes: 1, 30 kd, molecular weight markers; 2, 29 kd, Dac g 4-IP; 3, D. glomerata; 4, Agrostis alba; 5, Bromus arvensis; 6, Festuca elatior; 7, Anthoxanthum odoratum; 8, Holcus lanatus; 9, Lolium perenne; 10, Zea mays; 11, Pennisetum typho|Ad|fides; 12, Hordeum sativum; 13, Poa pratense; 14, Phleum pratense; 15, Secale cereale; 16, Alopecurus pratense; 17, Cynodon dactylon; 18, Artemisia vulgaris; 19, Ambrosia artemisaefolia; 20, Betula verrucosa; 21, Parietaria officinalis; 22, Quercus pedonculata; 23, Salix alba; 24, Capinus betulus; 25, Platanus hybrida; 26, Corylus avellana; 27, Tilia platyphyllos; 28, Populus nigra. Journal of Allergy and Clinical Immunology 1996 98, 1065-1072DOI: (10.1016/S0091-6749(96)80193-X) Copyright © 1996 Mosby, Inc. Terms and Conditions

FIG. 2 SDS-PAGE of SEs from different grass, weed, and tree pollens (A) silver-stained, followed by immunoblotting with biotinylated mAb H (B). Lanes: 1, 30 kd, molecular weight markers; 2, 29 kd, Dac g 4-IP; 3, D. glomerata; 4, Agrostis alba; 5, Bromus arvensis; 6, Festuca elatior; 7, Anthoxanthum odoratum; 8, Holcus lanatus; 9, Lolium perenne; 10, Zea mays; 11, Pennisetum typho|Ad|fides; 12, Hordeum sativum; 13, Poa pratense; 14, Phleum pratense; 15, Secale cereale; 16, Alopecurus pratense; 17, Cynodon dactylon; 18, Artemisia vulgaris; 19, Ambrosia artemisaefolia; 20, Betula verrucosa; 21, Parietaria officinalis; 22, Quercus pedonculata; 23, Salix alba; 24, Capinus betulus; 25, Platanus hybrida; 26, Corylus avellana; 27, Tilia platyphyllos; 28, Populus nigra. Journal of Allergy and Clinical Immunology 1996 98, 1065-1072DOI: (10.1016/S0091-6749(96)80193-X) Copyright © 1996 Mosby, Inc. Terms and Conditions

FIG. 3 Double-site immunoassay of Dac g 4 with mAb H as capture antibody and biotinylated mAb A. Group 4 allergens from four grass pollens (D. glomerata, Festuca elatior, Lolium perenne, and Phleum pratense) were compared with Dac g 4-IP. Results were expressed as percentage of maximal absorbance. Journal of Allergy and Clinical Immunology 1996 98, 1065-1072DOI: (10.1016/S0091-6749(96)80193-X) Copyright © 1996 Mosby, Inc. Terms and Conditions

FIG. 4 Inhibitory capacities of 11 grass pollen SEs compared with Dac g SE (100%). Relative contents of cross-reactive allergens were calculated from the protein concentration needed for 50% inhibition. Values are means of triplicate determinations. Normal human sera demonstrated no anti-Dac g 4 IgE-binding activity. Journal of Allergy and Clinical Immunology 1996 98, 1065-1072DOI: (10.1016/S0091-6749(96)80193-X) Copyright © 1996 Mosby, Inc. Terms and Conditions