HPLC TROUBLE SHOOTING Dr. Mirza Shahed Baig
Troubleshooting Locate the problem by ranking possible causes. There is no standard troubleshooting procedure. General Pattern: Locate the problem by ranking possible causes. Verify the presence of the most probable cause. If present – fix the problem, otherwise verify the existence of the next possible cause. First try to distinguish System problem or Method problem
HPLC SYSTEM
Method vs. System Troubleshooting System Parameters Flow stability Backpressure Clogging Detector problems Injection siutability Injection volume Temperature Method Parameters Flow rate Eluent type Eluent composition pH pH modifier (type) Gradient profile