Component-resolved diagnosis of baker's allergy based on specific IgE to recombinant wheat flour proteins∗ Ingrid Sander, PhD, Hans-Peter Rihs, PhD,

Slides:

Advertisements

Similar presentations

Peanut-specific IgE antibodies in asymptomatic Ghanaian children possibly caused by carbohydrate determinant cross-reactivity Abena S. Amoah, MSc, Benedicta.

Advertisements

Detection of novel occupational wood allergens in locust wood dust (Robinia pseudoacacia L.) Sabine Kespohl, PhD, Rolf Merget, MD, Axel Overlack, MD,

Serum granzymes and CD30 are increased in children's milk protein sensitive enteropathy and celiac disease Merja T. Augustin, MD, Jorma Kokkonen, MD,

Shea butter contains no IgE-binding soluble proteins

Repeated low-dose intradermal allergen injection suppresses allergen-induced cutaneous late responses Giuseppina Rotiroti, MD, Mohamed Shamji, PhD, Stephen.

Serum granzymes and CD30 are increased in children's milk protein sensitive enteropathy and celiac disease Merja T. Augustin, MD, Jorma Kokkonen, MD,

Birch pollen–related food allergy: Clinical aspects and the role of allergen-specific IgE and IgG4 antibodies Marija Geroldinger-Simic, MD, Thomas Zelniker,

Allergen immunotherapy: A practice parameter third update

High-titer IgE antibody specific for pollen allergens in northern California is associated with both wheezing and total serum IgE Elizabeth A. Erwin,

Kyoung-Bok Min, MD, PhD, Jin-Young Min, PhD

Jay A. Lieberman, MD, Faith R. Huang, MD, Hugh A

Santa Jeremy Ono, BA, PhD, Mark B. Abelson, MD

Ann-Marie M. Schoos, MD, PhD, Jacob D

Giant ragweed specific immunotherapy is not effective in a proportion of patients sensitized to short ragweed: Analysis of the allergenic differences.

Skin test diagnosis of grass pollen allergy with a recombinant hybrid molecule Carine Metz-Favre, MD, Birgit Linhart, PhD, Margarete Focke-Tejkl, PhD,

Delineating the specificity of an IgE-encoding transcriptome

Dorothy M. Ryan, MD, Stephen J. Fowler, MD, Robert M. Niven, MD

Birch pollen immunotherapy results in long-term loss of Bet v 1–specific TH2 responses, transient TR1 activation, and synthesis of IgE-blocking antibodies

Is 9 more than 2 also in allergic airway inflammation?

Firdaus Hamid, MD, PhD, Serge A. Versteeg, BSc, Aprilianto E

Shea butter contains no IgE-binding soluble proteins

In vivo allergenic activity of a hypoallergenic mutant of the major fish allergen Cyp c 1 evaluated by means of skin testing Nikolaos Douladiris, MD,

Component-resolved diagnosis of wheat flour allergy in baker's asthma

Lieuwe D. Bos, MSc, PhD, Peter J. Sterk, MD, PhD, Stephen J

Allergy testing in predicting outcome of open food challenge to peanut

Is there any role for allergen avoidance in the primary prevention of childhood asthma? Onno C.P. van Schayck, PhD, Tanja Maas, MSc, Janneke Kaper, PhD,

Simultaneous detection of total and allergen-specific IgE by using purified allergens in a fluorescent multiplex array Eva-Maria King, PhD, Lisa D. Vailes,

Peter M. Wolfgram, MD, David B. Allen, MD

Etiology of asthma exacerbations

Allergen microarray detects high prevalence of asymptomatic IgE sensitizations to tropical pollen-derived carbohydrates Clarissa R. Cabauatan, PhD, Christian.

Comparable IgE reactivity to natural and recombinant Api m 1 in cross-reactive carbohydrate determinant–negative patients with bee venom allergy Thilo.

Component resolution reveals additional major allergens in patients with honeybee venom allergy Julian Köhler, MS, Simon Blank, PhD, Sabine Müller, MD,

ImmunoCAP cellulose displays cross-reactive carbohydrate determinant (CCD) epitopes and can cause false-positive test results in patients with high anti-CCD.

Debra J. Palmer, PhD, Thomas R. Sullivan, BMa&CompSc(Hons), Michael S

Component-resolved diagnosis of kiwifruit allergy with purified natural and recombinant kiwifruit allergens Merima Bublin, PhD, Marina Pfister, MD, Christian.

Phillip Lieberman, MD, Michael Tankersley, MD

Genetically engineered hybrid proteins from Parietaria judaica pollen for allergen- specific immunotherapy Roberto González-Rioja, PhD, Ignacio Ibarrola,

Kerstin Bauermeister, MSc, Barbara K

Food allergy: A review and update on epidemiology, pathogenesis, diagnosis, prevention, and management Scott H. Sicherer, MD, Hugh A. Sampson, MD Journal.

Allergen-specific immunotherapy with recombinant grass pollen allergens Marek Jutel, MD, Lothar Jaeger, MD, Roland Suck, PhD, Hanns Meyer, Dipl Math,

Bites of the European pigeon tick (Argas reflexus): Risk of IgE-mediated sensitizations and anaphylactic reactions Jörg Kleine-Tebbe, MD, Anja Heinatz,

Increased sputum endotoxin levels are associated with an impaired lung function response to oral steroids in asthmatic patients Charles McSharry, PhD,

Component-resolved in vitro diagnosis of hazelnut allergy in Europe

Skin test evaluation of a novel peptide carrier–based vaccine, BM32, in grass pollen– allergic patients Verena Niederberger, MD, Katharina Marth, MD,

Autophagy: Nobel Prize 2016 and allergy and asthma research

Food allergy: Epidemiology, pathogenesis, diagnosis, and treatment

Advances in the approach to the patient with food allergy

Association between specific timothy grass antigens and changes in TH1- and TH2-cell responses following specific immunotherapy Véronique Schulten, PhD,

The Utility of Peanut Components in the Diagnosis of IgE-Mediated Peanut Allergy Among Distinct Populations Jay A. Lieberman, MD, Susanne Glaumann, MD,

Local allergic rhinitis: Allergen tolerance and immunologic changes after preseasonal immunotherapy with grass pollen Carmen Rondón, MD, PhD, Natalia.

Birch pollen–related food allergy: Clinical aspects and the role of allergen-specific IgE and IgG4 antibodies Marija Geroldinger-Simic, MD, Thomas Zelniker,

α-Purothionin, a new wheat allergen associated with severe allergy

Diagnosis of Alternaria alternata sensitization with natural and recombinant Alt a 1 allergens Juan A. Asturias, PhD, Ignacio Ibarrola, PhD, Angel Ferrer,

Ingrid Sander, PhD, Angelika Flagge, Rolf Merget, MD, Thomas M

Thunderstorm-related asthma attacks

Peanut-specific IgE antibodies in asymptomatic Ghanaian children possibly caused by carbohydrate determinant cross-reactivity Abena S. Amoah, MSc, Benedicta.

Päivi M. Salo, PhD, Samuel J. Arbes, DDS, MPH, PhD, Patrick W

Macrolide antibiotics and asthma treatment

Wheat lipid transfer protein is a major allergen associated with baker's asthma Arantxa Palacin, PhD, Santiago Quirce, MD, PhD, Alicia Armentia, MD, PhD,

Asthma: The past, future, environment, and costs

Repeated low-dose intradermal allergen injection suppresses allergen-induced cutaneous late responses Giuseppina Rotiroti, MD, Mohamed Shamji, PhD, Stephen.

Placental transfer of allergen-specific IgG but not IgE from a specific immunotherapy– treated mother Sabine Flicker, PhD, Katharina Marth, MD, Heinz.

Environmental factors and eosinophilic esophagitis

Peanut-specific IgE antibodies in asymptomatic Ghanaian children possibly caused by carbohydrate determinant cross-reactivity Abena S. Amoah, MSc, Benedicta.

The use of serum-specific IgE measurements for the diagnosis of peanut, tree nut, and seed allergy Jennifer M. Maloney, MD, Magnus Rudengren, BSc, Staffan.

Role of Art v 3 in pollinosis of patients allergic to Pru p 3

Carrier-bound, nonallergenic Ole e 1 peptides for vaccination against olive pollen allergy Teresa E. Twaroch, MSc, Margit Focke, PhD, Vera Civaj, Milena.

The effects of gastric digestion on codfish allergenicity

Allergy multivaccines created by DNA shuffling of tree pollen allergens Michael Wallner, PhD, Angelika Stöcklinger, MSc, Theresa Thalhamer, MSc, Barbara.

Natural history of cow’s milk allergy

Presentation transcript:

Component-resolved diagnosis of baker's allergy based on specific IgE to recombinant wheat flour proteins∗ Ingrid Sander, PhD, Hans-Peter Rihs, PhD, Gert Doekes, PhD, Santiago Quirce, MD, Esmeralda Krop, PhD, Peter Rozynek, MSc, Vera van Kampen, PhD, Rolf Merget, MD, Ursula Meurer, Thomas Brüning, MD, Monika Raulf, PhD Journal of Allergy and Clinical Immunology Volume 135, Issue 6, Pages 1529-1537 (June 2015) DOI: 10.1016/j.jaci.2014.11.021 Copyright © 2014 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 1 Total IgE and sIgE concentrations to grass pollen (gx1), wheat flour (f4), and rye flour (f5) of the study groups of 101 bakers and 29 control subjects. The median values of bakers (solid lines) and control subjects (dashed lines) and cutoff values (dotted lines) for sIgE (≥0.35 kUA/L) and total IgE (>100 kUA/L) are indicated. Journal of Allergy and Clinical Immunology 2015 135, 1529-1537DOI: (10.1016/j.jaci.2014.11.021) Copyright © 2014 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 2 sIgE concentrations of 0.1 kUA/L or greater in sera of bakers and control subjects to recombinant wheat allergens and the CCDs HRP and MUXF measured by using CAP-FEIA. The solid line marks the usual cutoff for positive values, and the dashed lines indicate the limits between the different CAP classes (class 1, ≥0.35-0.7 kUA/L; class 2, 0.7-3.5 kUA/L; class 3, 3.5-17.5 kUA/L; class 4, 17.5-50 kUA/L; and class 5, 50-100 kUA/L). Journal of Allergy and Clinical Immunology 2015 135, 1529-1537DOI: (10.1016/j.jaci.2014.11.021) Copyright © 2014 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 3 Inhibition of sIgE binding to single allergens by wheat flour, rye flour, or grass pollen proteins. Each bar indicates the mean and SD of the inhibition percentage obtained with 2 bakers' sera from different countries. Three sera for Tri a 39 and only 1 serum for Tri a 35 and the peroxidase were used, and in the case of Tri a 19, the second serum was a control serum. Inhibition greater than 20% is evaluated as significant. Journal of Allergy and Clinical Immunology 2015 135, 1529-1537DOI: (10.1016/j.jaci.2014.11.021) Copyright © 2014 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 4 sIgE concentrations to wheat flour in relation to the sum of sIgE concentrations to single allergens. The solid lines at 0.35 kUA/L mark the usual cutoff for positive values. Journal of Allergy and Clinical Immunology 2015 135, 1529-1537DOI: (10.1016/j.jaci.2014.11.021) Copyright © 2014 American Academy of Allergy, Asthma & Immunology Terms and Conditions