Osteoarthritis in a dish: the effects of pro-inflammatory cytokines on cartilage derived from induced pluripotent stem cells V.P. Willard, B.O. Diekman,

Slides:

Advertisements

Similar presentations

Full-thickness cartilage defects repaired by human induced pluripotent stem cells (IPSCS)-derived mesenchymal stem cells and plga scaffolds D. Shi, X.

Advertisements

Synergistic effects of progranulin and low intensity pulsed ultrasound on chondrocyte differentiation, migration and metabolism S.M. Uddin, B. Richbourgh,

Soluble factors from the notochordal-rich intervertebral disc inhibit endothelial cell invasion and vessel formation in the presence and absence of pro-inflammatory.

Full-thickness cartilage defects repaired by human induced pluripotent stem cells (IPSCS)-derived mesenchymal stem cells and plga scaffolds D. Shi, X.

Chondrocyte-Specific Loss of the Branched Actin Mediator ARP2/3 Results in Growth Plate Fusion and Proteoglycan Loss in Articular Cartilage B.O. Diekman,

Next-generation Sequencing Identifies Articular Cartilage and Subchondral Bone Mirnas after ESWT on Early Osteoarthritis Knee C.-J. Wang, J.-H. Cheng,

K.S. Santangelo Osteoarthritis and Cartilage

Aging-related inflammation in osteoarthritis

S. Gavard Osteoarthritis and Cartilage

Gene expression profiling for confirmation of effects of DNA polymeric molecules on in vitro arthritis model H.-J. Kim, A. Baek, M. Kim, S.-R. Cho Osteoarthritis.

Prospective economic evaluation of the costs for medications and nutraceuticals in knee osteoarthritis M. Chung, N. Zhao, J.B. Wong, C. Wang Osteoarthritis.

Characterization of cartilage repair in a focal mouse defect model

H.H. Lee, M.J. O'Malley, N.A. Friel, C.R. Chu

Prevention and treatment of intervertebral disc degeneration with bone marrow derived stem (stromal) cells – an in vivo study in sheep C.C. Shu, A. Dart,

Decreased proteoglycan degradation in IL-1β-treated cartilage co-cultured with TIMP-3- transduced cells J. Mason, A. Donahue, A. Yoskowitz, D. Richardson

Human synovial fluid derived mesenchymal stem cells expanded under low oxygen conditions and in a serum-free environment exhibit enhanced lineage-specific.

Role of endothelin-1 in endochondral ossification and osteoarthritis

Automatic cartilage segmentation and measurement for diagnosis of OA using 3D box and Gaussian filters X. Cui, S. Li, M. Yu, H. Kim, K.-S. Kwack, B.-H.

Effect of self assembled peptide-mesenchymal stem cell complex on delaying the progression of osteoarthritis S. Kim, S. Lee, Y. Jung, J. Kim, S. Kim

M.J. Martínez-Calatrava, R. Largo, G. Herrero-Beaumont

G. Venne, S. Pang, Y. Tse, R. Ellis Osteoarthritis and Cartilage

Change in bone area does not correlate with cartilage loss over 12 months in individuals with knee OA: Data from the osteoarthritis initiative M.A. Bowes,

Evaluation of TRB-n0224, a Chemically Modified Curcumin for the Treatment of Osteoarthritis R. Nixon, J. Coury, S.S. Shah, N. Chahine, T. Goldstein,

Z. Zhang, W. Liao, Z. Zhang, X. Duan, C. Hou

The differences on extracellular matrix among each portion of meniscus

The effect of conditional macrophage depletion on development of injury-induced knee OA in obese transgenic MAFIA mice C.-L. Wu, J. McNeill, K. Goon,

P. Yuan, X. Zhang, W. Yang, W. Kang, B. Yang, J. li, B. Chen, X. Li, B

Anti-inflammatory Properties of SM04690, a Small Molecule Inhibitor of the Wnt Pathway as a Potential Treatment for Knee Osteoarthritis V. Deshmukh,

M. Cucchiarini, H. Madry, E.F. Terwilliger

B. Vafaeian, S. Adeeb, M. El-Rich, J. Jaremko

Role of periostin and discoidin domain receptor-1 (DDR1) in the regulation of cartilage degeneration and expression of MMP-13 M. Attur, Q. Yang, T. Kirsch,

Role of TCF4 in signaling crosstalk with NFκB in human chondrocytes

The influence of early osteoarthritic collagen network de-structuring in articular cartilage on the tissue stiffness and swelling properties M. Nickien,

Bisphosphonate rescues cartilage from trauma damage by promoting mechanical sensitivity and calcium signaling in chondrocytes Y. Zhou, M. Park, L. Wang,

Mice with global deletion of mitogen inducible gene 6 display rapid and severe cartilage and subchondral bone damage after ligament and meniscus injury

Different responses of healthy, relative healthy and OA chondrocytes with IL1β treatment under hypoxia and normoxia X. Huang, L. Zhong, J. Post, M. Karperien

A new lipid formulation of low dose (1200mg) ibuprofen is non-inferior to high dose (2400mg) standard ibuprofen capsules in managing episodic knee pain.

M.N. Khan, M.Y. Ansari, T.M. Haqqi Osteoarthritis and Cartilage

A.L. McNulty, B.T. Estes, R.E. Wilusz, J.B. Weinberg, F. Guilak

M. Hufeland, M. Schünke, A.J. Grodzinsky, J. Imgenberg, B. Kurz

Synovial fluid levels of IL-6 and MMP'S in a mild equine LPS model

M.L. Roemhildt, A.E. Gauthier Osteoarthritis and Cartilage

Spontaneous home cage activity is sensitive to anti-NGF treatment or can be linked to joint stiffness in different models of surgery-induced osteoarthritis

Emerging role of endothelin receptor type B in regulating chondrogenic and hypertrophic changes of human mesenchymal stem cells M. Au, M. Zhu, W.-J.

Early intervention to prevent cartilage degeneration by administration of anti-vegf antibody in rabbit model T. Nagai, M. Sato, T. Kutsuna, T. Ukai,

Z. Huang, T. Stabler, F. Pei, V. Kraus Osteoarthritis and Cartilage

Development of growth factor tethered hyaluronan microspheres for in situ chondrogenic differentiation of human mesenchymal stem cells S. Ansboro, J.S.

In vitro release of corticosteroids in a new model of slow release platform in the treatment of osteoarthritis. S. Colen, M. van Rijen, S. Plomp, L.

Early Alterations in Articular Cartilage Surface topography in Post-traumatic Equine Model of Osteoarthritis S.M. Tabbaa, V.W. Wong, G. Silberman, C.W.

The interaction of lubricin/proteoglycan-4 (PRG4) with toll-like receptor 2: an anti- inflammatory role of PRG4 in synovial fluids from patients with osteoarthritis

R. L. Trevino, C. A. Pacione, S. Chubinskaya, A. -M. Malfait, M. A

A peptide temporally enhanced chondrogenesis of mesenchymal stem cells

Walking with increased foot progression angle can reduce joint compression in individuals with knee OA K. Manal, T.S. Buchanan Osteoarthritis and Cartilage

Monocyte chemoattractant protein 1 induced chondrocytes degeneration and cartilage degradation in osteoarthritis X. yuankun, K. Yan, W. Bin, L. Jian-Hao

Subjects with severe knee osteoarthritis reduce medio-lateral forces during gait at the expense of compressive knee contact forces S. Meireles, F. De.

Leptin signaling and body weight are independent mediators of spontaneous knee osteoarthritis in female rats J. Hudson, M. West, Y. Fu, T. Griffin Osteoarthritis.

K. -C. Wang, E. Kwan, K. Aris, T. T. Egelhoff, A. I. Caplan, J. F

Fracture severity predicts OA risk following intra-articular fractures

Imaging biomarker with T1 and T2 mappings in osteoarthritis – In vivo human articular cartilage study C. Yan, C. Wong, T. Li, N. Gong, Q. Chan, K. Chiu

Mitoprotection as a strategy to prevent chondrocyte death and cartilage degeneration following mechanical injury M.L. Delco, E.D. Bonnevie, H.H. Szeto,

How does lower leg alignment differ between soccer players, other athletes and non- athletic controls? W. colyn, R. Agricola, N. Arnout, J. Verhaar, J.

S. Brown, J. Pistiner, B. Sharma Osteoarthritis and Cartilage

The elastic modulus of articular cartilage at nano-scale and micro-scale measured using indentation type atomic force microscopy P. Rahnamay Moshtagh,

Knee kinematic and kinetic modifications under body weight unloading during overground walking at a constant speed A.G. Fischer, E.M. Debbi, A. Wolf

Adhesive barrier/directional controlled release for cartilage repair by endogenous progenitor cell recruitment G.-I. Im, J.-M. Lee, E.-A. Kim Osteoarthritis.

Discovery of a small molecule inhibitor of the Wnt pathway (SM04690) as a potential disease modifying treatment for knee osteoarthritis J.D. Hood, V.

K. -C. Wang, E. Kwan, K. Aris, T. T. Egelhoff, A. I. Caplan, J. F

A. Carlson, B. Bothner, R. June Osteoarthritis and Cartilage

General Information Osteoarthritis and Cartilage

Long-term effects of vitamin d supplementation and maintaining vitamin d sufficiency on knee osteoarthritis over 5 years B. Antony, S. Zheng, J. Burgess,

Presentation transcript:

Osteoarthritis in a dish: the effects of pro-inflammatory cytokines on cartilage derived from induced pluripotent stem cells V.P. Willard, B.O. Diekman, J. Sanchez-Adams, S.K. O'Connor, N. Christoforou, K.W. Leong, F. Guilak Osteoarthritis and Cartilage Volume 21, Pages S281-S282 (April 2013) DOI: 10.1016/j.joca.2013.02.590 Copyright © 2013 Terms and Conditions

Figure 1 Release of MMPs and GAGs from iPSC-derive cartilage in an in vitro model of OA. (A) All three IL-1α doses resulted in a significant increase in MMP release from iPSC neocartilage. (B) IL-1 α doses above 100 pg/ml resulted in an increased loss of GAG from the pellets and into the media. Groups not connected by the same letter as statistically different with P < 0.05, Mean ± SEM. Osteoarthritis and Cartilage 2013 21, S281-S282DOI: (10.1016/j.joca.2013.02.590) Copyright © 2013 Terms and Conditions

Figure 2 IL-1α induced degeneration of iPSC neocartilage. (A) Safranin-0 staining confirmed GAG loss from iPSC pellets at high IL-1α doses (positive staining is red, scale bar = 100 μm). (B) The compressive stiffness of iPSC-derived cartilage significantly decreased following IL-1α treatments above 100 pg/ml. Groups not connected by the same letter as statistically different with P < 0.05. Mean ±SEM. Osteoarthritis and Cartilage 2013 21, S281-S282DOI: (10.1016/j.joca.2013.02.590) Copyright © 2013 Terms and Conditions