Improved performance with saliva and urine as alternative DNA sources for malaria diagnosis by mitochondrial DNA-based PCR assays  C. Putaporntip, P.

Slides:



Advertisements
Similar presentations
Models for the organisation of hospital infection control and prevention programmes B. Gordts Clinical Microbiology and Infection Volume 11, Pages
Advertisements

Laboratory diagnosis and biosafety issues of biological warfare agents
A. Doménech-Sánchez, C. Juan, J.L. Pérez, C.I. Berrocal 
Validation of a four-primer real-time PCR as a diagnostic tool for single and mixed Plasmodium infections  L. Cnops, J. Jacobs, M. Van Esbroeck  Clinical.
B. Schulz, K. Weber, C. Radecke, C. Scheer, M. Ruhnke 
A. Doménech-Sánchez, C. Juan, J.L. Pérez, C.I. Berrocal 
Rapid and sensitive diagnosis of Toxoplasma gondii infections by PCR
Quantitative detection of Toxoplasma gondii DNA in human body fluids by TaqMan polymerase chain reaction  O. Kupferschmidt, D. Krüger, T.K. Held, H. Ellerhrok,
Effect of comprehensive validation of the template isolation procedure on the reliability of bacteraemia detection by a 16S rRNA gene PCR  A. Heininger,
Detection of fungal DNA in lysis–centrifugation blood culture for the diagnosis of invasive candidiasis in neonatal patients  L. Trovato, P. Betta, M.G.
Approach to diagnosis of infective endocarditis
Activity of quinupristin–dalfopristin in invasive isolates of Streptococcus pneumoniae from Italy  A. Pantosti, F. D'Ambrosio, E. Bordi, A. Scotto d'Abusco,
M. Cogliati, R. D'Amicis, A.M. Tortorano 
M.L. McMorrow, M. Aidoo, S.P. Kachur 
J.-P. Van geertruyden  Clinical Microbiology and Infection 
A comparison of three real-time PCR assays for the confirmation of Neisseria gonorrhoeae following detection of N. gonorrhoeae using Roche COBAS AMPLICOR 
O. Clerc, G. Greub  Clinical Microbiology and Infection 
G. Zhao, H. Zhai, Q. Yuan, S. Sun, T. Liu, L. Xie 
Prevalence of diarrheagenic Escherichia coli strains detected by PCR in patients with travelers' diarrhea  M. Vargas, J. Gascón, F. Gallardo, M. T. Jimenez.
M. Montes, D. Vicente, O. Esnal, G. Cilla, E. Pérez-Trallero 
Integron-mediated antibiotic multiresistance in Acinetobacter baumannii clinical isolates from Spain  J. Ruiz, M.M. Navia, C. Casals, J.M. Sierra, M.T.
A PCR-based method to differentiate between Acinetobacter baumannii and Acinetobacter genomic species 13TU  P.G. Higgins, H. Wisplinghoff, O. Krut, H.
Neonatal listeriosis in Algeria: the first two cases
New multiplex PCR method for the detection of Clostridium difficile toxin A (tcdA) and toxin B (tcdB) and the binary toxin (cdtA/cdtB) genes applied to.
Extraction of Chlamydia pneumoniae DNA from vascular tissue for use in PCR: an evaluation of four procedures  H.F. Berg, B. Maraha, A.M.C. Bergmans, A.
Evaluation of differential gene expression in susceptible and resistant clinical isolates of Klebsiella pneumoniae by DNA microarray analysis  A. Doménech-Sánchez,
Louis Pasteur, from crystals of life to vaccination
M. J. Röβler, G. Rappl, M. Muche, D. O. Hasselmann, W. Sterry, W
Rhodococcus equi pneumonia in a heart transplant recipient in Korea, with emphasis on microbial diagnosis  S.J. Yoo, H. Sung, J.D. Chae, M. -N. Kim, C.H.
A nested polymerase chain reaction for detection of Legionella pneumophila in clinical specimens  Sverker Bernander, Hanna-Stina Hanson, Bo Johansson,
Isolation and characterisation of toxin A-negative, toxin B-positive Clostridium difficile in Dublin, Ireland  D. Drudy, N. Harnedy, S. Fanning, R. O'Mahony,
Effect of comprehensive validation of the template isolation procedure on the reliability of bacteraemia detection by a 16S rRNA gene PCR  A. Heininger,
Vector control: a cornerstone in the malaria elimination campaign
Distribution of rpoB mutations among multidrug-resistant Mycobacterium tuberculosis (MDRTB) strains from Thailand and development of a rapid method for.
Breast milk causing neonatal sepsis and death
A comparison of three real-time PCR assays for the confirmation of Neisseria gonorrhoeae following detection of N. gonorrhoeae using Roche COBAS AMPLICOR 
A new multiplex PCR for easy screening of methicillin-resistant Staphylococcus aureus SCCmec types I–V  K. Boye, M.D. Bartels, I.S. Andersen, J.A. Møller,
Cohort study of the seasonal effect on nasal carriage and the presence of Mycobacterium leprae in an endemic area in the general population  M. Lavania,
Emergence of vancomycin-resistant enterococci in a tertiary hospital in Crete, Greece: a cluster of cases and prevalence study on intestinal colonisation 
Prevalence of the sat, set and sen genes among diverse serotypes of Shigella flexneri strains isolated from patients with acute diarrhoea  S.K. Niyogi,
Laboratory diagnosis and biosafety issues of biological warfare agents
Validation of an apicoplast genome target for the detection of Plasmodium species using polymerase chain reaction and loop mediated isothermal amplification 
E. López-Camacho, Z. Rentero, G. Ruiz-Carrascoso, J. -J. Wesselink, M
Prevalence of Streptococcus pneumoniae isolates bearing macrolide resistance genes in association with integrase genes of conjugative transposons in Japan 
Development and clinical validation of a molecular diagnostic assay to detect CTX-M- type β-lactamases in Enterobacteriaceae  J.D.D. Pitout, N. Hamilton,
Elements of design: the knowledge on which we build
Rapid diagnosis of coccidioidomycosis by nested PCR assay of sputum
Natural selection of K13 mutants of Plasmodium falciparum in response to artemisinin combination therapies in Thailand  C. Putaporntip, N. Kuamsab, R.
Metagenomics and probiotics
Prevalence of human papilloma virus type 5 DNA in lesional and non-lesional skin scales of Italian plaque-type psoriatic patients: association with disease.
Laboratory diagnosis of Clostridium difficile disease
R. Alonso, C. Muñoz, T. Peláez, E. Cercenado, M. Rodríguez-Creixems, E
A. Gelosia, L. Baldassarri, M. Deighton, T. van Nguyen 
Human isolates of Aeromonas possess Shiga toxin genes (stx1 and stx2) highly similar to the most virulent gene variants of Escherichia coli  A. Alperi,
A.P. Underwood, J. Green  Clinical Microbiology and Infection 
C. Wongsrichanalai, C.H. Sibley  Clinical Microbiology and Infection 
European guidelines for diagnosis and management of patients with suspected herpes simplex encephalitis  Miles Denton, Kevin G Kerr, Fraser Lewis  Clinical.
O. Clerc, G. Greub  Clinical Microbiology and Infection 
Pyrosequencing for rapid identification of carbapenem-hydrolysing OXA-type β- lactamases in Acinetobacter baumannii  T. Naas, L. Poirel, P. Nordmann  Clinical.
Comparison of one-tube multiplex PCR, automated ribotyping and intergenic spacer (ITS) sequencing for rapid identification of Acinetobacter baumannii 
K. Kaier, N.T. Mutters, U. Frank  Clinical Microbiology and Infection 
The first clinical isolate of Enterococcus faecium with the VanB phenotype in a teaching hospital in Greece  J. Papaparaskevas, J. Stefanou, A. Vatopoulos,
The atypical pneumonias: clinical diagnosis and importance
Abstracts Clinical Microbiology and Infection
Pathogen prevalence and blood meal identification in Amblyomma ticks as a means of reservoir host determination for ehrlichial pathogens  K.A. Pierce,
J.L. Balcázar  Clinical Microbiology and Infection 
Three years experience of real-time PCR for the diagnosis of Q fever
Typing of Clostridium difficile
Combination of pulsed-field gel electrophoresis (PFGE) and single-enzyme amplified fragment length polymorphism (SAFLP) for differentiation of multiresistant.
The future of diagnostic bacteriology
Presentation transcript:

Improved performance with saliva and urine as alternative DNA sources for malaria diagnosis by mitochondrial DNA-based PCR assays  C. Putaporntip, P. Buppan, S. Jongwutiwes  Clinical Microbiology and Infection  Volume 17, Issue 10, Pages 1484-1491 (October 2011) DOI: 10.1111/j.1469-0691.2011.03507.x Copyright © 2011 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 1 Representative 4% agarose gel electrophoresis of nested PCR targeting the mitochondrial cytochrome b gene of human malarial parasites and Plasmodium knowlesi. Specific amplifications were generated from secondary PCR with species-specific primers. Lane 1: 100-bp ladder marker. Lanes 2–6: Plasmodium falciparum (148 bp), Plasmodium malariae (138 bp), Plasmodium ovale (137 bp), Plasmodium vivax (181 bp) and P. knowlesi (131 bp), respectively. Lane 7: human DNA. Lane 8: water control. Clinical Microbiology and Infection 2011 17, 1484-1491DOI: (10.1111/j.1469-0691.2011.03507.x) Copyright © 2011 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 2 Relationship between parasite density and positive rates of Plasmodium falciparum (filled bars) and Plasmodium vivax (upward diagonal bars) by nested PCR targeting the mitochondrial cytochrome b gene (Cytb-PCR) of saliva (A), urine (B) and combined saliva and urine (C). Clinical Microbiology and Infection 2011 17, 1484-1491DOI: (10.1111/j.1469-0691.2011.03507.x) Copyright © 2011 European Society of Clinical Infectious Diseases Terms and Conditions

Feedback: Privacy Policy Feedback
Do Not Sell My Personal Information
About project: SlidePlayer Terms of Service

© 2025 SlidePlayer.com. Inc. All rights reserved.