Fluorescence.

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Presentation transcript:

Fluorescence

Topics Sensitivity (nM-pM) Derivatization LIF Contrast UV-vis measurements Derivatization LIF

Atomic Absorption Spectroscopy Elemental analysis Dissolved metals Source Hallow Cathode Lamp Cathode of specific element Sample Acetylene-air flame, nebulizer Detector PMT

Atomic absorption Gas-phase elemental atoms Narrow absorption lines (±0.01 nm) Element specific source Flame conditions control sensitivity 1-100 ppm (mg/L) varies with element

AA Spectrometer PMT Hollow cathode lamp Flame monochrometer Coating of element Air nebulizer Acetylene sample

Experiment Aspirate blank, set 100 % T Aspirate sample Matrix effects are common Standard addition analysis

Atomic Emission Spectroscopy