Details of Tune and Method Setup for LTQ Orbitrap

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Presentation transcript:

Details of Tune and Method Setup for LTQ Orbitrap Application: nanospray, peptides Gary Woffendin, Michaela Scigelova Thermo Electron, UK

Tune file - nanospray

Tune file - nanospray

Tune file - nanospray

Tune file - nanospray

Tune file - nanospray

Tune file - nanospray

Tune file - nanospray

Tune file - nanospray

Micro AS autosampler settings Note: Micro AS must be put back to ‘Menu/Serial’ (use the touch screen) Note: in sample table the vial position should be ‘1a1’

Micro AS autosampler settings Wash solution: water

Micro AS autosampler settings

Surveyor LC settings

Surveyor LC settings

LTQ Orbitrap settings

LTQ Orbitrap settings

LTQ Orbitrap settings

LTQ Orbitrap settings Exclusion duration set to about ½ peak width (here 15 sec)

LTQ Orbitrap settings

LTQ Orbitrap settings

LTQ Orbitrap settings

LTQ Orbitrap settings

LTQ Orbitrap settings Scan event 2 – MS/MS of the 1st most intense ion

LTQ Orbitrap settings Scan event 3 – MS/MS of the 2nd most intense ion

LTQ Orbitrap settings Scan event 4 – MS/MS of the 3rd most intense ion

Divert Valve

Method Summary

Method Summary