Messenger ribonucleic acid for the gonadal luteinizing hormone/human chorionic gonadotropin receptor is not present in human endometrium  Elizabeth A Stewart, M.D., Marine Sahakian, M.D., Alan Rhoades, B.A., Bradley J Van Voorhis, M.D., Romana A Nowak, Ph.D.  Fertility and Sterility  Volume 71, Issue 2, Pages 368-372 (February 1999) DOI: 10.1016/S0015-0282(98)00453-1

FIGURE 1 Schematic diagram of the human LH/hCG receptor and the primer set used to amplify the cDNA for the receptor. This receptor has the classic structure of a seven-transmembrane–spanning, G-coupled receptor. The cell membrane is shown as a lipid bilayer. All primer sets amplify the extracellular region, except for the xantho primer pair, which amplifies transmembrane segments 2–6 wholly within exon 11. Fertility and Sterility 1999 71, 368-372DOI: (10.1016/S0015-0282(98)00453-1)

FIGURE 2 Polymerase chain reaction products visualized on a 1% agarose gel after two rounds of amplification with the LH primer set. A 647-bp fragment is seen in the lane containing granulosa cell cDNA (GC); no bands are seen in the lanes containing yeast cDNA (Y) as a negative control or in the five separate endometrial samples (E1-E5). The darkest band of the 100-bp ladder (LAD) represents the 800-bp fragment and is indicated by an arrow. The other 20 endometrial samples amplified produced no bands. Fertility and Sterility 1999 71, 368-372DOI: (10.1016/S0015-0282(98)00453-1)

FIGURE 3 Polymerase chain reaction products visualized on a 1% agarose gel after amplification with the xantho primer set. A 524-bp fragment is seen in the lane containing granulosa cell cDNA (GC), and no bands are seen in the lane containing yeast cDNA (Y) as a negative control. Bands are seen in four of the five endometrial samples (all except E3). A 100-bp ladder (LAD) is seen, with the darkest band representing an 800-bp fragment and indicated by an arrow. Bands were seen in 15 of the remaining 20 endometrial samples amplified. Sequencing confirmed the identity of the products. Fertility and Sterility 1999 71, 368-372DOI: (10.1016/S0015-0282(98)00453-1)