Conversion of the CD4+ T cell profile from TH2-dominant type to TH1-dominant type after varicella-zoster virus infection in atopic dermatitis Takao Fujimura,

Slides:

Advertisements

Similar presentations

Immunopathogenesis of human gastrointestinal infection by Anisakis simplex Victoria del Pozo, PhDa, Ignacio Arrieta, MDa, Teresa Tuñon, MD, PhDb, Isabel.

Advertisements

Topical application of a vitamin D3 analogue and corticosteroid to psoriasis plaques decreases skin infiltration of TH17 cells and their ex vivo expansion

Different expression of cytokine and membrane molecules by circulating lymphocytes on acute mental stress in patients with atopic dermatitis in comparison.

Glucocorticoids inhibit chemokine generation by human eosinophils

Interleukin-4 receptor expression by human B cells: Functional analysis with a human interleukin-4 toxin, DAB389IL-4 Haifa H. Jabara, MSca, Donata Vercelli,

Interleukin-4 receptor expression by human B cells: Functional analysis with a human interleukin-4 toxin, DAB389IL-4 Haifa H. Jabara, MSca, Donata Vercelli,

Role of γ/δ T cells in a patient with CD4+ CD3– lymphocytosis, hypereosinophilia, and high levels of IgE Ilan Bank, MDa,d, Avner Reshef, MDb, Miriam.

Isolation and characterization of a clone encoding a major allergen (Bla g Bd90K) involved in IgE-mediated cockroach hypersensitivity Ricki Helm, PhDa,

Cell-to-cell contact between activated CD4+ T lymphocytes and unprimed monocytes interferes with a TH1 response Miriam Wittmann, MD, Mareike Alter, Tanja.

The environmental pollutant pyrene induces the production of IL-4

Christine M. Braun, BA, Shau-Ku Huang, PhD, Gregory G

Factors affecting the cytokine production of human T cells stimulated by different modes of activation Yoshio Harada, MDa, Sumiko Watanabe, PhDa, Hans.

Differential cytokine induction by the human skin–associated autoallergen thioredoxin in sensitized patients with atopic dermatitis and healthy control.

Predominance of type 2 cytokine–producing CD4+ and CD8+ cells in patients with atopic dermatitis Masatoshi Nakazawa, DVMa, Nakako Sugi, MDb, Hiroshi.

Signal transduction pathways triggered by the FcϵRIIb receptor (CD23) in human monocytes lead to nuclear factor-κB activation Rosa M. Ten, MD, PhDa,

Topical application of a vitamin D3 analogue and corticosteroid to psoriasis plaques decreases skin infiltration of TH17 cells and their ex vivo expansion

Expression and Role of IL-15 in Post-Burn Hypertrophic Scars

CD40-mediated p38 mitogen-activated protein kinase activation is required for immunoglobulin class switch recombination to IgE Ke Zhang, MD, PhD, Ling.

Allergen-specific IgE production of committed B cells from allergic patients in vitro Peter Steinberger, MSca, Barbara Bohlea, Franco di Padova, MDb,

Eniko Sonkoly, MD, Anja Muller, MD, Antti I

Prostaglandin E2 control of T cell cytokine production is functionally related to the reduced lymphocyte proliferation in atopic dermatitis Sai Chan,

Enhanced nasal cytokine production in human beings after in vivo challenge with diesel exhaust particles David Diaz-Sanchez, PhD, Albert Tsien, MD, Adrian.

Fisetin, a flavonol, inhibits TH2-type cytokine production by activated human basophils Shinji Higa, MDa, Toru Hirano, MDa, Mayumi Kotani, BScb, Motonobu.

Expression of IL-6, IL-8, and RANTES on human bronchial epithelial cells, NCI-H292, induced by influenza virus A Satoshi Matsukura, MD, Fumio Kokubu,

Kathleen R. Bartemes, BA, Gail M. Kephart, BS, Stephanie J

Eosinophil peroxidase stimulates the release of granulocyte-macrophage colony- stimulating factor from bronchial epithelial cells Shinji Motojima, MDa,

Glucocorticoids inhibit IL-4 and mitogen-induced IL-4Rα chain expression by different posttranscriptional mechanisms Lourdes Mozo, PhDa, Abel Gayo, BSa,

Christian Möbs, Thomas Schmidt Journal of Investigative Dermatology

Direct Evidence to Support the Role of Antigen-Specific CD8+ T Cells in Melanoma- Associated Vitiligo Frédérique-Anne Le Gal, Philippe Lefebvre, Jean-Christophe.

Chronologic analysis of in situ cytokine expression in mite allergen-induced dermatitis in atopic subjects Nobuo Yamada, MDa, Motoshi Wakugawa, MDa,

Elastin Peptides Induce Migration and Terminal Differentiation of Cultured Keratinocytes Via 67 kDa Elastin Receptor in Vitro: 67 kDa Elastin Receptor.

The latex allergen Hev b 5 transcript is widely distributed after subcutaneous injection in BALB/c mice of its DNA vaccine Jay E. Slater, MDa, Elizabeth.

Noritaka Oyama, Keiji Iwatsuki, Yoshimi Homma, Fumio Kaneko

Interleukin-13 Receptor in Psoriatic Keratinocytes: Overexpression of the mRNA and Underexpression of the Protein Juan C. Cancino-Díaz, Elba Reyes-Maldonado,

Evidence for expression of eosinophil-associated IL-12 messenger RNA and immunoreactivity in bronchial asthma Esra Nutku, MDa, Abdelilah Soussi Gounni,

Christopher L. Kepley, PhDa, John C. Cambier, PhDb, Penelope A

Glucocorticoids inhibit chemokine generation by human eosinophils

Chronic fatigue syndrome: Identification of distinct subgroups on the basis of allergy and psychologic variables Larry Borish, MDa, Karen Schmaling,

The relative contribution of IL-4 and IL-13 to human IgE synthesis induced by activated CD4+ or CD8+ T cells Juha Punnonen, MD, PhD, Hans Yssel, PhD,

Mukesh Kumar, PhDa, Aruna K. Behera, PhDa, Jianan Hu, MDa, Richard F

Delayed eosinophil programmed cell death in vitro: A common feature of inhalant allergy and extrinsic and intrinsic atopic dermatitis Bettina Wedi, MD,

Inflammatory cells, cytokine and chemokine expression in asthma immunocytochemistry and in situ hybridization Qutayba Hamid, MD, PhD, Editor Journal.

Cell surface characterization of T lymphocytes and allergen-specific T cell clones: Correlation of CD26 expression with T H1 subsets Martin Willheim,

Heterogeneity of mast cells and T cells in the nasal mucosa

IL-12 affects Dermatophagoides farinae–induced IL-4 production by T cells from pediatric patients with mite-sensitive asthma Takeshi Noma, MD, PhD, Izumi.

Ganglioside GQ1b enhances Ig production by human PBMCs

Rachel L. Miller, MDa, Thomas M

IL-9 expression by human eosinophils: Regulation by IL-1β and TNF-α

Antigen-specific and nonspecific determinants of cytokine production during topical sensitization of mice to chemical allergens Artin Moussavi, PhDa,

TH1/TH2 cytokines and inflammatory cells in skin biopsy specimens from patients with chronic idiopathic urticaria: Comparison with the allergen-induced.

David M. Essayan, MD, Charity C

Intracellular IL-5 and T-lymphocyte subsets in atopic and nonatopic bronchial asthma Yutaro Shiota, MD, PhDa, Hitomi Arikita, MTa, Naokatsu Horita, MDb,

Harald Renz, MD, Chaya Brodie, PhD, Katherine Bradley, BS, Donald Y. M

Blood eosinophils from atopic donors express messenger RNA for the α, β, and γ subunits of the high-affinity IgE receptor (FcϵRI) and intracellular, but.

Murine model of atopic dermatitis associated with food hypersensitivity Xui-Min Li, MDa, Gary Kleiner, MD, PhDa, Chin-Kang Huang, MSa, Soo Yung Lee, MDa,

Immunochemical characterization of recombinant and native tropomyosins as a new allergen from the house dust mite, Dermatophagoides farinae Tsunehiro.

A thiol antioxidant regulates IgE isotype switching by inhibiting activation of nuclear factor-κB Yukiyoshi Yanagihara, PhD, Yuji Basaki, MSc, Keiichi.

Chemical constituents of diesel exhaust particles induce IL-4 production and histamine release by human basophils Gilles Devouassoux, MDa, Andrew Saxon,

Pia J. Hauk, MDa, Qutayba A. Hamid, MD, PhDc, George P

Rame A. Taha, MDa, Eleanor M. Minshall, PhDa, Donald Y. M

Identification of a Commonly Used CDR3 Region of Infiltrating T Cells Expressing Vβ13 and Vβ15 Derived From Psoriasis Patients Ha Young Hwang, Young.

Lactic acid bacteria inhibit TH2 cytokine production by mononuclear cells from allergic patients Pierre Pochard, PhDab, Philippe Gosset, PhDb, Corinne.

IL-4– and IL-5–positive T lymphocytes, eosinophils, and mast cells in allergen-induced late-phase cutaneous reactions in atopic subjects Luis T. Barata,

Mechanisms of inhibition of IgE synthesis by nedocromil sodium: Nedocromil sodium inhibits deletional switch recombination in human B cells Richard K.S.

Transcriptional control of the IL-5 gene by human helper T cells: IL-5 synthesis is regulated independently from IL-2 or IL-4 synthesis Akio Mori, MD,

Reciprocal regulation of cultured human mast cell cytokine production by IL-4 and IFN-γ Hiroshi Tachimoto, MD, PhDa, Motohiro Ebisawa, MD, PhDa,b, Tomohide.

IL-31 is associated with cutaneous lymphocyte antigen–positive skin homing T cells in patients with atopic dermatitis Janine Bilsborough, PhD, Donald.

Plant defense–related enzymes as latex antigens

Diisocyanate antigen–enhanced production of monocyte chemoattractant protein-1, IL- 8, and tumor necrosis factor-α by peripheral mononuclear cells of workers.

Keiji Miyazawa, MSc, Akio Mori, MD, PhD, Hirokazu Okudaira, MD, PhD

Presentation transcript:

Conversion of the CD4+ T cell profile from TH2-dominant type to TH1-dominant type after varicella-zoster virus infection in atopic dermatitis Takao Fujimura, PhDa, Rika Yamanashi, BSca, Mikio Masuzawa, MD, PhDa, Yuhsuke Fujita, MDa, Kensei Katsuoka, MD, PhDa, Sigeo Nishiyama, MD, PhDa, Masao Mitsuyama, MD, PhDb, Kikuo Nomoto, MD, PhDc Journal of Allergy and Clinical Immunology Volume 100, Issue 2, Pages 274-282 (August 1997) DOI: 10.1016/S0091-6749(97)70236-7 Copyright © 1997 Mosby, Inc. Terms and Conditions

Fig. 1 Cytokine profiles of AD lesions. Erythematous skin lesions of AD were examined for cytokine mRNA expression by means of RT-PCR. Total RNA extracted from each biopsy specimen was reverse transcribed to cDNA. cDNA for β-actin, CD3δ, IL-2, IFN-γ, IL-4, and IL-5 was amplified by PCR. DNA size markers (øX174/HaeIII digest) are shown in last lane on right in both panels. Predicted sizes of PCR products for β-actin, CD3 δ, IL-2, IFN-γ, IL-4, and IL-5 were 548, 316, 266, 356, 317, and 405 bp, respectively. Journal of Allergy and Clinical Immunology 1997 100, 274-282DOI: (10.1016/S0091-6749(97)70236-7) Copyright © 1997 Mosby, Inc. Terms and Conditions

Fig. 2 Cytokine profiles in atopic skin lesions after these patients contracted varicella. Atopic skin lesions subsequently improved. Total RNA extracted from each biopsy specimen was reverse transcribed to cDNA. cDNAs for β-actin, CD3δ, IL-2, IFN-γ, IL-4, and IL-5 were amplified by PCR. Predicted sizes of PCR products for β-actin, CD3 δ, IL-2, IFN-γ, IL-4, and IL-5 were 548, 316, 266, 356, 317, and 405 bp, respectively. Expression patterns of cytokines before contracting varicella are shown in lanes 1 to 3 of Fig. 1. Journal of Allergy and Clinical Immunology 1997 100, 274-282DOI: (10.1016/S0091-6749(97)70236-7) Copyright © 1997 Mosby, Inc. Terms and Conditions

Fig. 3 Enumeration of IL-4– and IL-5–producing cells by ELISPOT assay. PBMCs obtained from A, three AD patients showing improvement after varicella (cases 1 to 3) and from B, three healthy volunteers as controls (cont 1 to 3) were stimulated with 10 μg mite antigen/ml for 48 hours. After cells were washed, serial dilutions of cell suspension were transferred to nylon-based plates that had been coated with anti-IL-4 or anti-IL-5 mAb, and further incubated for 24 hours. Plates were then washed and incubated with anti-IL-4 or anti-IL-5 polyclonal antibodies. Plates were again washed and incubated with peroxidase-conjugated 2nd antibody. Spots representing IL-4– or IL5–producing cells were visualized by adding 3-amino-9-ethylcarbazole in 0.1 mol/L phosphate-citrate buffer. Data are expressed as mean of three wells ± SD. *p < 0.05, **p < 0.001 p-value compared with control (none) every individual case. Journal of Allergy and Clinical Immunology 1997 100, 274-282DOI: (10.1016/S0091-6749(97)70236-7) Copyright © 1997 Mosby, Inc. Terms and Conditions

Fig. 3 Enumeration of IL-4– and IL-5–producing cells by ELISPOT assay. PBMCs obtained from A, three AD patients showing improvement after varicella (cases 1 to 3) and from B, three healthy volunteers as controls (cont 1 to 3) were stimulated with 10 μg mite antigen/ml for 48 hours. After cells were washed, serial dilutions of cell suspension were transferred to nylon-based plates that had been coated with anti-IL-4 or anti-IL-5 mAb, and further incubated for 24 hours. Plates were then washed and incubated with anti-IL-4 or anti-IL-5 polyclonal antibodies. Plates were again washed and incubated with peroxidase-conjugated 2nd antibody. Spots representing IL-4– or IL5–producing cells were visualized by adding 3-amino-9-ethylcarbazole in 0.1 mol/L phosphate-citrate buffer. Data are expressed as mean of three wells ± SD. *p < 0.05, **p < 0.001 p-value compared with control (none) every individual case. Journal of Allergy and Clinical Immunology 1997 100, 274-282DOI: (10.1016/S0091-6749(97)70236-7) Copyright © 1997 Mosby, Inc. Terms and Conditions

Fig. 4 Enumeration of IL-4– and IL-5–producing cells by ELISPOT assay. PBMCs obtained from three AD patients showing improvement after varicella (cases 1 to 3) were stimulated with 100 PFU VZV/ml for 48 hours, and IL-4– and IL-5–producing cells were counted as described for Fig. 3. Data are expressed as mean of three wells ± SD. *p < 0.05, **p < 0.001 p-value compared with control (none) every individual case. Journal of Allergy and Clinical Immunology 1997 100, 274-282DOI: (10.1016/S0091-6749(97)70236-7) Copyright © 1997 Mosby, Inc. Terms and Conditions

Fig. 4 Enumeration of IL-4– and IL-5–producing cells by ELISPOT assay. PBMCs obtained from three AD patients showing improvement after varicella (cases 1 to 3) were stimulated with 100 PFU VZV/ml for 48 hours, and IL-4– and IL-5–producing cells were counted as described for Fig. 3. Data are expressed as mean of three wells ± SD. *p < 0.05, **p < 0.001 p-value compared with control (none) every individual case. Journal of Allergy and Clinical Immunology 1997 100, 274-282DOI: (10.1016/S0091-6749(97)70236-7) Copyright © 1997 Mosby, Inc. Terms and Conditions

Fig. 5 Enumeration of IFN-γ–producing cells by ELISPOT assay. PBMCs obtained from three AD patients showing improvement after varicella (cases 1 to 3) were stimulated with 10 μg/ml mite antigen or 100 PFU/ml VZV or mixing both for 48 hours. IFN-γ producing cells were counted as described for Fig. 3. Data are expressed as mean of three wells ± SD. *p < 0.05, **p < 0.001 p-value compared with control (none) every individual case. Journal of Allergy and Clinical Immunology 1997 100, 274-282DOI: (10.1016/S0091-6749(97)70236-7) Copyright © 1997 Mosby, Inc. Terms and Conditions

Fig. 6 PCR detection of mRNA for IL-12 p35 and p40 in varicella lesions. Skin biopsy specimens were obtained from lesions within 4 days after appearance of vesicles (lanes 1 to 8) and from crusting lesions 7 to 10 days after appearance of vesicles (lanes 9 and 10). RNA from each varicella lesion was reverse transcribed into cDNA and amplified by PCR with primers specific for β-actin and IL-12 p35 and p40. DNA size markers (øX174/HaeIII digest) are shown in last lane on right. PCR products for IL-12 p35 and p40 cDNA were transferred to nylon membranes and hybridized to an internal 32P-labeled probe. Journal of Allergy and Clinical Immunology 1997 100, 274-282DOI: (10.1016/S0091-6749(97)70236-7) Copyright © 1997 Mosby, Inc. Terms and Conditions

Fig. 7 IL-12 p40 mRNA production induced by VZV. PBMCs from atopic or nonatopic donors were cocultured for 5 hours in medium alone or medium containing VZV. RNA was reverse transcribed into cDNA and amplified by PCR with primers specific for β-actin and IL-12 p40. DNA size markers (øX174/HaeIII digest) are shown in last lane on right. PCR products for IL-12 p40 cDNA were transferred to nylon membranes and hybridized to an internal 32P-labeled probe. Even-numbered lanes show results for VZV-stimulated cells. Odd-numbered lanes, show results for nonstimulated cells. A, PBMCs from atopic donors. B, PBMCs from nonatopic donors. Journal of Allergy and Clinical Immunology 1997 100, 274-282DOI: (10.1016/S0091-6749(97)70236-7) Copyright © 1997 Mosby, Inc. Terms and Conditions

Fig. 7 IL-12 p40 mRNA production induced by VZV. PBMCs from atopic or nonatopic donors were cocultured for 5 hours in medium alone or medium containing VZV. RNA was reverse transcribed into cDNA and amplified by PCR with primers specific for β-actin and IL-12 p40. DNA size markers (øX174/HaeIII digest) are shown in last lane on right. PCR products for IL-12 p40 cDNA were transferred to nylon membranes and hybridized to an internal 32P-labeled probe. Even-numbered lanes show results for VZV-stimulated cells. Odd-numbered lanes, show results for nonstimulated cells. A, PBMCs from atopic donors. B, PBMCs from nonatopic donors. Journal of Allergy and Clinical Immunology 1997 100, 274-282DOI: (10.1016/S0091-6749(97)70236-7) Copyright © 1997 Mosby, Inc. Terms and Conditions