Comparative lipidomic analysis of synovial fluid in human and canine osteoarthritis  M.K. Kosinska, S.C. Mastbergen, G. Liebisch, J. Wilhelm, R.B. Dettmeyer, B. Ishaque, M. Rickert, G. Schmitz, F.P. Lafeber, J. Steinmeyer  Osteoarthritis and Cartilage  Volume 24, Issue 8, Pages 1470-1478 (August 2016) DOI: 10.1016/j.joca.2016.03.017 Copyright © 2016 Osteoarthritis Research Society International Terms and Conditions

Fig. 1 The concentrations of lipid classes in normal and OA SF from the knee joints of nine dogs. Lipids were quantified by ESI/MS-MS as described in Materials and methods. Data are presented as medians with interquartile ranges. P-values less than 0.05 were considered significant. P-values marked with * are less than 0.001. PC(O)-phosphatidylcholine + ether phosphatidylcholine, LPC-lysophosphatidylcholine, PI-phosphatidylinositol, SM-sphingomyelin. Osteoarthritis and Cartilage 2016 24, 1470-1478DOI: (10.1016/j.joca.2016.03.017) Copyright © 2016 Osteoarthritis Research Society International Terms and Conditions

Fig. 2 The concentrations of PC (A and B) and PC O (C) species in normal and OA SF from the knee joints of nine dogs. Lipid species were quantified by ESI/MS-MS as described in Materials and methods. Ether species were assigned based on the assumption that the PCs contain only fatty acids with an even number of carbon atoms. Data are presented as medians with interquartile ranges. P-values less than 0.05 were considered significant. P-values marked with * are less than 0.001. PC-phosphatidylcholine, PC O-ether phosphatidylcholine. Osteoarthritis and Cartilage 2016 24, 1470-1478DOI: (10.1016/j.joca.2016.03.017) Copyright © 2016 Osteoarthritis Research Society International Terms and Conditions

Fig. 3 The concentrations of LPC (A), and SM (B and C) species in normal and OA SF from the knee joints of nine dogs. Lipid species were quantified by ESI/MS-MS as described in Materials and methods. SM species were assigned based on the assumption of a sphingoid base with two hydroxyl groups. Data are presented as medians with interquartile ranges. P-values less than 0.05 were considered significant. P-values marked with * are less than 0.001. LPC-lysophosphatidylcholine, SM-sphingomyelin. Osteoarthritis and Cartilage 2016 24, 1470-1478DOI: (10.1016/j.joca.2016.03.017) Copyright © 2016 Osteoarthritis Research Society International Terms and Conditions

Fig. 4 The levels of lipid classes in SF from the canine OA model compared with the early and late stages of human OA. Knee SF from early human OA (n = 17), human donors with healthy joints (n = 9), late human OA (n = 13), and nine dogs with OA and healthy control knees were analyzed by ESI-MS/MS as described in Materials and methods. The data for lipid classes from human and canine OA SF are presented as relative differences to the average values from healthy human control SF (=1) respectively to the canine contralateral control SF values (=1). Data are expressed as medians with interquartile ranges. P-values less than 0.05 were considered significant: P-values marked with * are less than 0.001. PC(O)-phosphatidylcholine + ether phosphatidylcholine, LPC-lysophosphatidylcholine, SM-sphingomyelin. Osteoarthritis and Cartilage 2016 24, 1470-1478DOI: (10.1016/j.joca.2016.03.017) Copyright © 2016 Osteoarthritis Research Society International Terms and Conditions

Fig. 5 The levels of PC (A and B), and PC O (C) species in SF from canine OA model compared with the early and late stages of human OA. Knee SF from early human OA (n = 17), late human OA (n = 13), human donors with healthy joints (n = 9), and nine dogs with OA and healthy contralateral control knees were analyzed by ESI-MS/MS as described in Materials and methods. Ether species were assigned based on the assumption that the PCs contain only fatty acids with an even number of carbon atoms. The data obtained for lipid classes from human and canine OA SF are presented as relative differences to average values from healthy human control SF (=1) respectively to the canine contralateral control SF values (=1). Data are expressed as medians with the interquartile ranges. P-values less than 0.05 were considered significant. P-values marked with * are less than 0.001. PC-phosphatidylcholine, PC O-ether phosphatidylcholine. Osteoarthritis and Cartilage 2016 24, 1470-1478DOI: (10.1016/j.joca.2016.03.017) Copyright © 2016 Osteoarthritis Research Society International Terms and Conditions

Fig. 6 The levels of LPC (A), and SM (B and C) species in SF from canine OA model compared with the early and late stages of human OA. Knee SF from early human OA (n = 17), late human OA (n = 13), human donors with healthy joints (n = 9), and nine dogs with OA and healthy contralateral control knees were analyzed by ESI-MS/MS as described in Materials and methods. SM species were assigned based on the assumption of a sphingoid base with two hydroxyl groups. The data obtained for lipid classes from human and canine OA SF are presented as relative differences to values obtained from healthy control SF (=1) of the same species. Data are expressed as medians with interquartile ranges. P-values less than 0.05 were considered significant. P-values marked with * are less than 0.001. LPC-lysophosphatidylcholine, SM-sphingomyelin. Osteoarthritis and Cartilage 2016 24, 1470-1478DOI: (10.1016/j.joca.2016.03.017) Copyright © 2016 Osteoarthritis Research Society International Terms and Conditions

Fig. 7 The levels of PC (A and B), PC O (C), LPC (D), and SM (E and F) species in normal canine SF compared with SF from healthy human joints. Knee SF from human donors with healthy joints (n = 9) and from healthy canine joints (n = 9) were analyzed by ESI-MS/MS as described in Materials and methods. Species annotation is based on the assumptions shown in Figs. 2 and 3. The data obtained for lipid species from canine contralateral normal SF are presented as relative differences to average values obtained from SF of healthy human joints (=1). Data are expressed as medians with interquartile ranges. P-values less than 0.05 were considered significant. P-values marked with * are less than 0.001. PC-phosphatidylcholine, PC O-ether phosphatidylcholine, LPC-lysophosphatidylcholine, SM-sphingomyelin. Osteoarthritis and Cartilage 2016 24, 1470-1478DOI: (10.1016/j.joca.2016.03.017) Copyright © 2016 Osteoarthritis Research Society International Terms and Conditions