miR‐10b locus is hypermethylated in breast cancer. A

Slides:

Advertisements

Similar presentations

Trends Biomedical Science

Advertisements

Figure S1 (a) Maspin promotor methylation status before and after treatment with 5-aza-dC (5 µM) in C33A cells visualized with a MSP. (b) C13ORF18 promotor.

Epigenetic Regulation of Cancer Stem Cell Genes in Triple-Negative Breast Cancer Naofumi Kagara, Kelly T. Huynh, Christine Kuo, Hideyuki Okano, Myung.

Expression of Snail1 correlates with the level of LSD1 and CoREST in tumour tissues. Expression of Snail1 correlates with the level of LSD1 and CoREST.

Induction of mPer1 mRNA expression by prostaglandin E2 (PGE2) in NIH3T3 cells. Induction of mPer1 mRNA expression by prostaglandin E2 (PGE2) in NIH3T3.

KIT Is a Frequent Target for Epigenetic Silencing in Cutaneous Melanoma Christina Dahl, Cecilie Abildgaard, Rikke Riber-Hansen, Torben Steiniche, Johanne.

CBP and p300 are recruited to MYC target genes.

JNK is activated by Wnt5a.

Expression analyses of KLK7 mRNA in the brains of AD patients

MiR‐10b* down‐regulates the expression of BUB1, PLK1 and CCNA2 proteins in breast cancer cell lines.A.SPIN‐ordered expression matrix of miR‐10b* and its.

Volume 133, Issue 6, Pages (December 2007)

Characterization of transgenic mice.

Interaction with Snail1 is required for LSD1 recruitment to the E‐cadherin promoter in vivo. Interaction with Snail1 is required for LSD1 recruitment to.

Epigenetic Inhibition of Nuclear Receptor Small Heterodimer Partner Is Associated With and Regulates Hepatocellular Carcinoma Growth Nan He, Kyungtae.

High-throughput analysis of informative CpG sites for the four studied tumor suppressor genes (A-D). High-throughput analysis of informative CpG sites.

Reactive oxygen species inhibit miR‐199a and miR‐125b expression levels in vitro and in vivo. Reactive oxygen species inhibit miR‐199a and miR‐125b expression.

MiR‐199a directly targets ERBB2 and ERBB3, whereas miR‐125b targets ERBB3. miR‐199a directly targets ERBB2 and ERBB3, whereas miR‐125b targets ERBB3. (A)

Demethylation of a nonpromoter cytosine-phosphate-guanine island in the aromatase gene may cause the aberrant up-regulation in endometriotic tissues

Volume 26, Issue 4, Pages (October 2014)

Histone Modifications Associated with Somatic Hypermutation

Expression of PD‐1 on PBMC and malignant exudate T cells

The upstream region of MUC5AC bound by SPDEF is required for the expression of MUC5AC in human lung cancer cells The upstream region of MUC5AC bound by.

Volume 42, Issue 4, Pages (April 2005)

Volume 13, Issue 2, Pages (October 2015)

NRP2 induces expression of GLI1. A,B

ZEB1‐dependent epigenetic modifications ASchemes for the genomic loci of the miRNA and E‐cadherin genes, showing regions of the CpG islands (yellow), of.

Chromatin regulation upon recruitment of HOTAIR

KIT Is a Frequent Target for Epigenetic Silencing in Cutaneous Melanoma Christina Dahl, Cecilie Abildgaard, Rikke Riber-Hansen, Torben Steiniche, Johanne.

Volume 17, Issue 8, Pages (November 2016)

Histone deacetylase 1 protein depletion affects general histone acetylation and specific gene expression. Histone deacetylase 1 protein depletion affects.

HMGB1 reverses mitochondrial DNA damage caused by mutant Atxn1 The mitochondrial DNA amplification assay with cerebellar tissue revealed that mitochondrial.

Association of AIM2, RIG‐I and NLRP3 inflammasomes with better survival in NPC patients.A.Overexpression of ASC, caspase‐1 and IL‐1β proteins as well as.

The ERK pathway mediates the effect of EGF on LIMT, which normally inhibits mammary cell migration and invasion The ERK pathway mediates the effect of.

TGF‐β1 treatment induces the chromatin binding of Smad2/3/4 in 4T1 cells TGF‐β1 treatment induces the chromatin binding of Smad2/3/4 in 4T1 cells A, BWestern.

Histone Modifications Associated with Somatic Hypermutation

MET inhibition promotes p21 nuclear translocation

Characterization of MET‐addicted cells rendered resistant to MET tyrosine kinase inhibitors Characterization of MET‐addicted cells rendered resistant to.

MiR‐146b induces 3T3‐L1 adipocyte differentiation qRT‐PCR analysis of miR‐146b during 3T3‐L1 differentiation (n = 4). miR‐146b induces 3T3‐L1 adipocyte.

Core promoter methylation in mediators of adipogenesis.

Volume 46, Issue 5, Pages (June 2012)

Limited transcriptomic changes upon HOTAIR RNA overexpression in MDA‐MB‐231 breast cancer cells Limited transcriptomic changes upon HOTAIR RNA overexpression.

Epigenetic Control of the S100A6 (Calcyclin) Gene Expression

The human GPR109A promoter is methylated and GPR109A expression is silenced in human colon carcinoma cells. The human GPR109A promoter is methylated and.

Features of the LIMT gene and RNA abundance in cell lines of different tissues of origin Features of the LIMT gene and RNA abundance in cell lines of different.

Volume 27, Issue 5, Pages (September 2007)

ADAM8 knockdown profoundly decreases orthotopic tumor formation, CTC spread and metastasis A–DStable ADAM8 KD (shA8) clones were characterized in vitro.

Favorable safety profile of LV‐mediated intracerebral gene therapy

Genomewide profiling of chromatin accessibility in prostate cancer specimens Genomewide profiling of chromatin accessibility in prostate cancer specimens.

Functional Modulation of IGF-Binding Protein-3 Expression in Melanoma

Lidocaine and ropivacaine, but not bupivacaine, demethylate deoxyribonucleic acid in breast cancer cells in vitro P. Lirk, M.W. Hollmann, M. Fleischer,

Tangle formation and downregulation of miR‐132‐3p in LOAD A‐G

Dnmt2 mutants show delayed immune responses and Dnmt2–EGFP relocalizes and interacts with DCV RNA during infection. Dnmt2 mutants show delayed immune responses.

ADAM8 is induced by hypoxia and promotes angiogenesis ASixteen h after plating, cells were cultured under normoxic (−) or hypoxic (+, 1% O2) conditions.

Study design Study design DNA and total RNA of DCM patients were isolated from left ventricular myocardial biopsies and peripheral blood. Next‐generation.

Knocking down Wnt3 increases the cells' response to trastuzumab and reduces cells' invasiveness. Knocking down Wnt3 increases the cells' response to trastuzumab.

Volume 25, Issue 11, Pages e6 (December 2018)

Volume 41, Issue 2, Pages (January 2011)

FBXL19-AS1 acts as a sponge of miR-431-5p in lung cancer (A) The predicted binding sites of FBXL19-AS1 and miR-431-5p, and the mutant sites in mutant-type.

(A) Relative expression levels of the top most down-regulated genes in LATS2L breast tumors (TCGA-BRCA dataset, see Fig 1) in the panel of breast cancer.

Methylation status of IGFBPL1 in human breast cancer.

CDCP1 is required for invadopodia formation and ECM degradation by human breast cancer cells. CDCP1 is required for invadopodia formation and ECM degradation.

Epigenetic regulation of p16INK4a in human gastric cancer.

DNA hypermethylation silences TRAIL in transformed cells.

A, high-resolution methylation analysis of ZNF545 promoter by BGS in representative carcinoma cell lines. A, high-resolution methylation analysis of ZNF545.

Specific DNA methylation and expression signatures associated with binding of E2A–PBX1 fusion protein. Specific DNA methylation and expression signatures.

Comparison of the frequency of nucleotide variation in the mtDNA D-loop region between stomach and other tumor cell lines. Comparison of the frequency.

DNA demethylation reactivates GSN expression in gastric cancer cells.

Cell lines with aberrant expression of NRG1 are exquisitely sensitive to downregulation of ERBB3 signaling. Cell lines with aberrant expression of NRG1.

Number of identical D-loop DNA clones detected in 10 DNA clones derived from liver tumor tissues. Number of identical D-loop DNA clones detected in 10.

MTB1 to MTB3 Are Direct Transcriptional Targets of MYC2.

Presentation transcript:

miR‐10b locus is hypermethylated in breast cancer. A miR‐10b locus is hypermethylated in breast cancer.A.Schematic representation of the miR‐10b and miR‐139‐5p genomic loci. miR‐10b locus is hypermethylated in breast cancer.A.Schematic representation of the miR‐10b and miR‐139‐5p genomic loci. The analysed CpG islands are indicated (solid lines).B.MeDIP analysis on breast cancer cell lines was performed as described in the text. Immunoprecipitated genomic DNA from MCF7 cell line was quantified by using qPCR with TaqMan assays directed to miR‐10b* CpG islands #1 and #2 and miR‐139‐5p CpG Island #1.C.MeDIP experiment of MCF7 cells treated with 5‐aza‐dC. The percentage of enrichment over the INPUT of both CpG island #1 and #2 diminishes in the cells treated with the demethylating agent. NT = not treated cells.D,E.RT‐qPCR analysis of pri‐miR‐10b (D), mature miR‐10b* and miR‐10b (E) expression was performed in MCF7 breast cancer cells treated with the demethylating agent 5‐aza‐dC versus untreated cells (NT).F.Bisulphite sequencing results from four matched tumoural and peritumoural breast patient samples. Results were averaged for each CpG position, whereby the number of investigated clones is presented between parenthesis and the shading of each circle represents the percentage of methylation as indicated. Relative positions of nucleotides inside the CpG islands are indicated.GMeDIP experiments performed on genomic DNA extracted from breast cancer tissues. MeDIP were carried out with antibody directed against 5‐methylcitidine in two breast cancer and two reductive mammoplasty samples. N = reductive mammoplasty. The immunoprecipitated genomic DNA was quantified by qPCR using TaqMan assays amplifying miR‐10b* CpG Island #1, CpG Island #2 and miR‐139‐5p CpG Island #1. Relative enrichments are presented as percentages of the input DNA used for the immunoprecipitations. Francesca Biagioni et al. EMBO Mol Med. 2012;4:1214-1229 © as stated in the article, figure or figure legend