Quiz#2 LC710 10/15/12 name____________ Q1(8 %) You have identified this protein conserved across 4 species (non-rodent). MNGTYEMRATLSMLWAAYMFLLIML * MQGTYEMRASLTMLWAAYMFLLVML * MQGTYEMRASLSMIWAAYMFLLIML * MQGTYEMKASLSMLWAAYMFLLIML * * = STOP You make an antibody to this protein and find that it is found only in the eyes of all 4 species 1 2 1 2 1 2 1 2 You eventually want to identify this gene in mouse. How will you start? Using _______________________as a PCR template (2%) ? Design 5’ to 3’ oligos that will give you the best chance at identifying some of this coding sequence in the mouse. You are fearful of getting to much garbage from your amplification! Codon Probability In fish 1 In mouse 2 organisms (1%) Oligo1:5’_______________________________ (1%) Oligo2: 5’_______________________________ (4%) How will you know that Your PCR succeeded in amplifying the correct fragment (be specific)? _____________________________________ _____________________________________ _____________________________________