Long-lasting protection against nasal challenge with influenza virus 252 d after a single DNA vaccination. Long-lasting protection against nasal challenge.

Slides:

Advertisements

Similar presentations

Immune Parameters Correlate with Protection Against Ebola Virus Infection in Rodents and Nonhuman Primates by Gary Wong, Jason S. Richardson, Stéphane.

Advertisements

Volume 5, Issue 3, Pages (March 2002)

Antitumor effect of the combination of IL-2 IC and anti–CTLA-4.

Identification of combination treatment–responsive dysfunctional tumor-infiltrating CD8+ T cell population. Identification of combination treatment–responsive.

Serum IgG (A–D) and IgA (E–H) titer from BALB/c mice (n = 5/group) at day 0 or following COBRA P1 virus i.n., i.p., or i.m. prime at day 21 or following.

Volume 25, Issue 6, Pages (June 2017)

Antiviral activity of Chongkukjang extracts against influenza A virus in vitro and in vivo Bai Wei, Se-Yeoun Cha, Min Kang, Young Jin Kim, Chang-Won Cho,

Roles of systemic versus tumour SK1 in the regulation of local tumour growth and lung colonization/metastasis.A.Effects of siRNA‐mediated SK1 knockdown.

Fig. 5. Correlation of tail and long bone growth velocities with Cxm serum concentrations in mice. Correlation of tail and long bone growth velocities.

Characterization of the targeting units within the vaccine proteins.

In vivo prophylactic and therapeutic efficacy of C12G6 in mice

Targeted DNA vaccination induced protection against nasal challenge with influenza virus 14 d after DNA vaccination. Targeted DNA vaccination induced protection.

(A) Weight loss curves following intranasal infection of different mouse groups with COBRA, seasonal, or pandemic influenza viruses. (A) Weight loss curves.

HA-specific Abs in serum 14 d after a single vaccination with the various targeted DNA vaccines. HA-specific Abs in serum 14 d after a single vaccination.

A single DNA vaccination of C57BL/6 mice induced Ab responses of IgG1 and/or IgG2c subclass. A single DNA vaccination of C57BL/6 mice induced Ab responses.

Serum IgG subclass representation from BALB/c mice (n = 5/group) following COBRA P1 virus i.n., i.p., or i.m. prime followed by i.p. or i.m. boost. Serum.

Virus-specific serum antibody response of BALB/c mice to DNA prime followed by VRP boost. Virus-specific serum antibody response of BALB/c mice to DNA.

Volume 26, Issue 2, Pages (February 2018)

Protection from influenza virus challenge.

Prf1−/− mice exhibit increased immunopathology with prior CD8 T cell memory secondary to immunization. Prf1−/− mice exhibit increased immunopathology with.

Viral Diseases How do vaccines work?.

Virus-specific serum antibody response of BALB/c mice to DNA prime followed by VRP boost. Virus-specific serum antibody response of BALB/c mice to DNA.

Survival of BALB/c mice after HK/Syd challenge.

Sleep/wake phenotype of S551D mutant mice.

Serum neutralizing antibody response of BALB/c mice to DNA prime followed by VRP boost. Serum neutralizing antibody response of BALB/c mice to DNA prime.

Indomethacin worsens the effects of C. difficile infection in mice.

Fig. 3. H3N2 incidence forecasts based on the cluster model for the Unites States. H3N2 incidence forecasts based on the cluster model for the Unites States.

Volume 25, Issue 9, Pages (September 2017)

Comparison of therapeutic efficacies of C12G6 and other bnAbs in mice

Inflammatory APC show highest expression of TNF superfamily ligands in the lung after LCMV infection. Inflammatory APC show highest expression of TNF superfamily.

Romain Gallet et al. BTS 2016;1:14-28

Fig. 4. Efficacy of C12G6 compared with and in combination with oseltamivir in mice. Efficacy of C12G6 compared with and in combination with oseltamivir.

Urine ammonia is highly correlated with plasma K

Elevated androgens in global ERα−/− mice decrease lung ILC2 numbers and diminish the KLRG1− ILC2 subset. Elevated androgens in global ERα−/− mice decrease.

Ag 85A–specific immune responses.

Fig. 6 In utero injection of inflammatory cytokines or adoptive transfer of activated T cells leads to pregnancy loss. In utero injection of inflammatory.

Unchanged worm burden and microfilaremia in basophil-deficient mice compared with their basophil-competent littermates. Unchanged worm burden and microfilaremia.

Chimeric HA-based universal influenza virus vaccine concept.

NKTfh cell–driven class switch against polysaccharide Ag is IL-21-influenced. NKTfh cell–driven class switch against polysaccharide Ag is IL-21-influenced.

CD160 on CD8+ T cells is required for optimal clearance of L

Nrf2−/− mice suffered greater renal damage by STZ compared with Nrf2+/+ mice. Nrf2−/− mice suffered greater renal damage by STZ compared with Nrf2+/+ mice.

Normalized weight gain of protein-sufficient and -deficient pigs after HRV inoculation and HIFM transplantation monitored over a period of 4 weeks. Normalized.

Anti-hOX40L treatment prevents aGVHD development in NOG mice transplanted with human PBMCs. NOG mice received human PBMCs (1 × 107) via tail vein. animals.

Schematic representation of the immunization regimens used in this study. Schematic representation of the immunization regimens used in this study. BALB/c.

Prime and boosts with PBK001 vaccine provided 100% protection with low bacterial burden in organs. Prime and boosts with PBK001 vaccine provided 100% protection.

CD8 T cell memory alters the immune profile in enhanced HLH without altering viral load. CD8 T cell memory alters the immune profile in enhanced HLH without.

Fig. 1. CAR4 and CAR8 cells demonstrate in vitro and in vivo antileukemic efficacy. CAR4 and CAR8 cells demonstrate in vitro and in vivo antileukemic efficacy.

Fig. 4 Administration of BMS to mice inhibits autoimmune-relevant pharmacodynamic endpoints driven by IL-12 and IFNα. Administration of BMS

Fig. 5 Treatment with BMS (PO BID) protects from wasting and colitis in two SCID mouse models. Treatment with BMS (PO BID) protects from.

Intranasal immunization of mice with S

Fig. 3 Local Maraba treatment of TNBC tumors provides long-term systemic protection. Local Maraba treatment of TNBC tumors provides long-term systemic.

Functional characterization of multidonor class antibodies.

Fig. 1. The HCN channel blocker ivabradine (IVA) is analgesic in a mouse model of type 1 diabetes. The HCN channel blocker ivabradine (IVA) is analgesic.

Fig. 6 Multivalent DNA vaccine and protein antigen delivery by T4-AAV nanoparticles. Multivalent DNA vaccine and protein antigen delivery by T4-AAV nanoparticles.

Fig. 4 Dox-CBD-SA treatment shows reduced toxicity.

Fig. 3 Echocardiographic analyses of cardiac function.

Sang Kyun Ahn, Vanessa Tran, Andrea Leung, Mark Ng, Ming Li, Jun Liu

LAIV and WT influenza virus infection similarly enhance 19F pneumococcal carriage density and duration of colonization. LAIV and WT influenza virus infection.

Fig. 3 Mmp-2−/− mice are protected from obesity and leptin resistance.

Fig. 3 Plumage brightness (PC1) for each sex in relation to morphological, ecological, and behavioral traits. Plumage brightness (PC1) for each sex in.

Subodh Verma et al. BTS 2018;3:

In vivo prophylactic and therapeutic efficacy of C12G6 in mice

IL-17A−/− mice are more susceptible to intravaginal HSV-2 challenge following intranasal immunization. IL-17A−/− mice are more susceptible to intravaginal.

Fig. 1. GDF15 is up-regulated with obesity, and AAV-GDF15 improves metabolic parameters in DIO mice. GDF15 is up-regulated with obesity, and AAV-GDF15.

The PI3Kβ inhibitor enhances the antitumor activity of T cell–mediated immunotherapy in mice bearing PTEN loss tumors. The PI3Kβ inhibitor enhances the.

Moderate-affinity vaccine antigens elicited greatest antitumor response. Moderate-affinity vaccine antigens elicited greatest antitumor response. Wild-type.

Fig. 3 Superiority of BAFF-R versus CD19-CAR T cells in a Burkitt lymphoma model is not due to greater tumor antigen density. Superiority of BAFF-R versus.

In the absence of TLR5 and inflammasome recognition, FliC’s D2/D3 domains are essential for secondary IgG1 and IgG2c anti-flagellin responses. In the absence.

Fig. 3 Ect2 gene inactivation lowers cardiomyocyte endowment and is lethal in mice. Ect2 gene inactivation lowers cardiomyocyte endowment and is lethal.

Fig. 2. In vivo local CD25-targeted NIR-PIT induces regression of treated LL/2-luc tumors. In vivo local CD25-targeted NIR-PIT induces regression of treated.

Presentation transcript:

Long-lasting protection against nasal challenge with influenza virus 252 d after a single DNA vaccination. Long-lasting protection against nasal challenge with influenza virus 252 d after a single DNA vaccination. (A) Mice were vaccinated i.m. with 25 μg DNA in each quadriceps and 252 d later challenged with 5xLD50 of influenza virus A (PR8/H1N1, n = 6 per group). Weight was monitored for 10 d. Weight loss of 20% was defined as humane endpoint (* p < 0.05, **p < 0.01, ***p < 0.001, two-way ANOVA). For reasons of clarity only some groups are shown, see (B) for all groups. (B) The % body weight on day 6 for all the different groups. The results were analyzed with Mann–Whitney two-tailed t test, where *p < 0.05, **p < 0.01, ***p < 0.001 compared with the nontargeted αNIP-HA vaccine. (C) Mean weight on day 6 versus % survival after challenge with influenza A (PR8/H1N1). Correlation analyzed using Pearson correlation two-tailed test. Color-coding as in Fig. 3. Ranveig Braathen et al. ImmunoHorizons 2018;2:38-53 Copyright © 2018 The Authors