Differential transcriptomic profile in spermatozoa achieving pregnancy or not via ICSI Sandra García-Herrero, Nicolás Garrido, José Antonio Martínez-Conejero,

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Presentation transcript:

Differential transcriptomic profile in spermatozoa achieving pregnancy or not via ICSI Sandra García-Herrero, Nicolás Garrido, José Antonio Martínez-Conejero, José Remohí, Antonio Pellicer, Marcos Meseguer Reproductive BioMedicine Online Volume 22, Issue 1, Pages 25-36 (January 2011) DOI: 10.1016/j.rbmo.2010.09.013 Copyright © 2010 Reproductive Healthcare Ltd. Terms and Conditions

Figure 1 Flowchart for microarray experiments. DET=differentially expressed transcript; EET=exclusively expressed transcript; ICSI =intracytoplasmic sperm injection. Reproductive BioMedicine Online 2011 22, 25-36DOI: (10.1016/j.rbmo.2010.09.013) Copyright © 2010 Reproductive Healthcare Ltd. Terms and Conditions

Figure 2 Proportions of differentially expressed transcripts within gene ontology terms in the pregnant group with fresh spermatozoa. Numbers in parentheses indicate the number within each term. BP=biological processes; CC=cellular components; MF=molecular functions. Reproductive BioMedicine Online 2011 22, 25-36DOI: (10.1016/j.rbmo.2010.09.013) Copyright © 2010 Reproductive Healthcare Ltd. Terms and Conditions

Figure 3 Proportions of exclusively expressed transcripts within gene ontology terms in the pregnant and not pregnant groups with fresh spermatozoa. Numbers in parentheses indicate the number within each term. BP=biological processes; CC=cellular components; ICSI=intracytoplasmic sperm injection; MF=molecular functions; NP=not pregnant; P=pregnant. Reproductive BioMedicine Online 2011 22, 25-36DOI: (10.1016/j.rbmo.2010.09.013) Copyright © 2010 Reproductive Healthcare Ltd. Terms and Conditions

Figure 4 Proportions of exclusively expressed transcripts within gene ontology terms in the pregnant and not pregnant groups with frozen spermatozoa. Numbers in parentheses indicate the number within each term. BP=biological processes; CC=cellular components; ICSI=intracytoplasmic sperm injection; MF=molecular functions; NP=not pregnant; P=pregnant. Reproductive BioMedicine Online 2011 22, 25-36DOI: (10.1016/j.rbmo.2010.09.013) Copyright © 2010 Reproductive Healthcare Ltd. Terms and Conditions

Figure 5 Proportions of exclusively expressed transcripts within gene ontology terms in the pregnant and not pregnant groups with fresh and frozen spermatozoa with a possible reproductive role. Numbers in parentheses indicate the number within each term. NP=not pregnant; P=pregnant. Reproductive BioMedicine Online 2011 22, 25-36DOI: (10.1016/j.rbmo.2010.09.013) Copyright © 2010 Reproductive Healthcare Ltd. Terms and Conditions

Figure 6 Microarray validation. FC=fluorescence; qPCR=quantitative real-time PCR results. Reproductive BioMedicine Online 2011 22, 25-36DOI: (10.1016/j.rbmo.2010.09.013) Copyright © 2010 Reproductive Healthcare Ltd. Terms and Conditions