Gene expression of inflammatory mediators in different chambers of the human heart Guro Valen, MD, PhD, Gabrielle Paulsson, PhD, Anna M Bennet, Göran.

Slides:

Advertisements

Similar presentations

Date of download: 5/28/2016 Copyright © The American College of Cardiology. All rights reserved. From: Expression, activity and functional significance.

Advertisements

The effect of cardiotomy suction on the brain injury marker S100β after cardiopulmonary bypass Russell E Anderson, MD, PhD, Lars-Olof Hansson, MD, PhD,

Perioperative Statin Therapy for Patients Undergoing Coronary Artery Bypass Grafting Amr F. Barakat, MD, Marwan Saad, MD, PhD, Ahmed Abuzaid, MD, Amgad.

Cardiovascular Risk and Psoriasis: Beyond the Traditional Risk Factors

Lucas R. Barbieri, MD, Domingos D. Lourenço-Filho, MD, PhD, Elaine R

Perioperative Statin Therapy for Patients Undergoing Coronary Artery Bypass Grafting Amr F. Barakat, MD, Marwan Saad, MD, PhD, Ahmed Abuzaid, MD, Amgad.

An unusual case of severe aortic valve stenosis in an adult caused by aortic valve inflammatory myofibroblastic tumor Dong Zhao, MD, Chunsheng Wang,

Prevention of ischemia-reperfusion injury in cardiac surgery: Therapeutic strategies targeting signaling pathways Kay Maeda, MD, PhD, Marc Ruel, MD,

George D. Webb, PhD, Lay Har Lim, BSc, Vernon M. S

Time course and cellular localization of inducible nitric oxide synthases expression during cardiac allograft rejection Neil K Worrall, MD, Thomas P.

Cardiac surgery increases the activity of matrix metalloproteinases and nitric oxide synthase in human hearts Irvin Mayers, MD, Thomas Hurst, MVSc, Lakshmi.

Vein Graft Harvesting Induces Inflammation and Impairs Vessel Reactivity Kazuhiro Hinokiyama, MD, Guro Valen, MD, PhD, Shinichi Tokuno, MD, PhD, Jenny.

Inhibitory Effects of Calcitonin Gene-Related Peptides on Experimental Vein Graft Disease Xiaoning Zhang, MD, Jian Zhuang, MD, Hongsui Wu, MB, Zhihong.

Interleukin-17 and Prostaglandin E2 Are Involved in Formation of an M2 Macrophage- Dominant Microenvironment in Lung Cancer Lunxu Liu, MD, PhD, Dongxia.

Pediatric End-Stage Failing Hearts Demonstrate Increased Cardiac Stem Cells Brody Wehman, MD, Sudhish Sharma, PhD, Rachana Mishra, PhD, Yin Guo, PhD,

Y. Higashimoto, M. Ohata, Y. Yamagata, T. Iwata, M. Masuda, T

Angiotensin II receptor type 1 is upregulated in atrial tissue of patients with rheumatic valvular disease with atrial fibrillation Hongliang Cong, MD,

High-mobility-group box protein 1 A box reduces development of sodium laurate- induced thromboangiitis obliterans in rats Xiangqian Kong, MD, Hai Yuan,

Identifying and Treating COPD in Cardiac Patients

Postmortem and ex vivo carbon monoxide ventilation reduces injury in rat lungs transplanted from non–heart-beating donors Boming Dong, MD, PhD, Paul.

Yiping Wang, Yuet-Ching Tay, David C.H. Harris Kidney International

Time-dependent cellular population of textured-surface left ventricular assist devices contributes to the development of a biphasic systemic procoagulant.

Malignant B-Cell Lymphoma Arising in a Large, Left Atrial Myxoma

Cytokines Link Toll-Like Receptor 4 Signaling to Cardiac Dysfunction After Global Myocardial Ischemia John Cha, MD, Zhiping Wang, MD, Lihua Ao, BS, Ning.

Modulation of the expression of tissue plasminogen activator and its inhibitor by hypoxia in human peritoneal and adhesion fibroblasts Ghassan M Saed,

Novel Bioresorbable Vascular Graft With Sponge-Type Scaffold as a Small-Diameter Arterial Graft Tadahisa Sugiura, MD, PhD, Shuhei Tara, MD, PhD, Hidetaka.

Michael Frenken, MD, Arno Krian, MD The Annals of Thoracic Surgery

Ischemic preconditioning is not cardioprotective in senescent human myocardium Babett Bartling, PhD, Ivar Friedrich, MD, Rolf-E Silber, MD, Andreas Simm,

Expression and Function of Endothelial Nitric Oxide Synthase Messenger RNA and Protein Are Higher in Internal Mammary Than in Radial Arteries Guo-Wei.

Michael G. Katz, MD, PhD, Anthony S. Fargnoli, PhD, Andrew P

Daniel R. Meldrum, MD, Brian D

Transbronchial gene transfer of endothelial nitric oxide synthase to transplanted lungs Anders Jeppsson, MD, Carlo Pellegrini, MD, Timothy O’Brien, MD,

Nebulized nitrite protects rat lung grafts from ischemia reperfusion injury Toshihiro Okamoto, MD, PhD, Xiaoying Tang, MS, Allison Janocha, BS, Caral.

Ventricular thrombus and subarachnoid bleeding during support with ventricular assist devices Eva M Gruber, MD, Rainald Seitelberger, MD, Peter Mares,

Dexamethasone Pretreatment Provides Antiinflammatory and Myocardial Protection in Neonatal Arterial Switch Operation Ruth Heying, MD, PhD, Edith Wehage,

Glucocorticoids reduce ischemia-reperfusion-induced myocardial apoptosis in immature hearts Jeffrey M Pearl, MD, David P Nelson, MD, PhD, Steven M Schwartz,

William T. Mahle, MD, Emilia Matthews, MS, Kirk R. Kanter, MD, Brian E

Carbon monoxide–saturated preservation solution protects lung grafts from ischemia– reperfusion injury Junichi Kohmoto, MD, Atsunori Nakao, MD, Ryujiro.

Human cytokine responses to coronary artery bypass grafting with and without cardiopulmonary bypass Martin Strüber, MD, Jochen T Cremer, MD, Bernhard.

Activation of Endothelial and Coagulation Systems in Left Ventricular Assist Device Recipients Ranjit John, MD, Sandhya Panch, MBBS, Jennifer Hrabe,

Echocardiographic evidence of right ventricular remodeling after transplantation Holger K Eltzschig, MD, Tomislav Mihaljevic, MD, John G Byrne, MD, Raila.

Endothelial Cell Injury in Cardiovascular Surgery: The Systemic Inflammatory Response1 Edward M Boyle, MD, Timothy H Pohlman, MD, Marion C Johnson, MD,

Emergency Management of Severe Right Ventricular Inflow Obstruction Secondary to a Metastatic Cardiac Tumor Erdinç Naseri, MD, Bulent Eralp, MD, Ibrahim.

Coronary surgery without cardiotomy suction and autotransfusion reduces the postoperative systemic inflammatory response Martin Westerberg, MD, Anders.

Volume 61, Issue 1, Pages (January 2002)

Correction Journal of Vascular Surgery

Combination extract inhibits immune responses in vivo.

Expression of Endothelin-1 and Constitutional Nitric Oxide Synthase Messenger RNA in Saphenous Vein Endothelial Cells Exposed to Arterial Flow Shear Stress

Increased cerebral and renal endothelial nitric oxide synthase gene expression after cardiopulmonary bypass in the rat C. David Mazer, MD, Francoise.

Right lung ischemia induces contralateral pulmonary vasculopathy in an animal model Edouard Sage, MD, Olaf Mercier, MD, Philippe Herve, MD, Ly Tu, PhD,

Importance of endothelial nitric oxide synthase for the hypothermic protection of lungs against ischemia-reperfusion injury Li Zhang, MD, Sanjeev Kumar,

Infant cardiopulmonary bypass: a procoagulant state

Double-Outlet Right Atrium: Anatomic and Clinical Considerations

Unusual Cause of Femorofemoral Cardiopulmonary Bypass Failure

Endothelial Nitric Oxide Synthase in Bicuspid Aortic Valve Disease

Drew-Anderson technique attenuates systemic inflammatory response syndrome and improves respiratory function after coronary artery bypass grafting Josef.

Ronald A. Bronicki, MD, Carl L. Backer, MD, Harris P

Time course of proinflammatory and anti-inflammatory responses after cardiac operation: monocyte HLA-DR expression Y.John Gu, MD, PhD, Willem van Oeveren,

Mesenteric dysfunction after cardiopulmonary bypass: role of complement C5a Motohisa Tofukuji, MD, PhD, Gregory L Stahl, PhD, Caroline Metais, MD, Mikio.

Aprotinin Enhances the Endogenous Release of Interleukin-10 After Cardiac Operations Gary E Hill, Robert P Diego, Alfred H Stammers, Suzanne M Huffman,

Isolated tricuspid regurgitation caused by a dilated tricuspid annulus

Effect of Vein Graft Harvesting on Endothelial Nitric Oxide Synthase and Nitric Oxide Production Michael R. Dashwood, PhD, Kay Savage, PhD, Audrey Dooley,

Fungal Purulent Constrictive Pericarditis in a Heart Transplant Patient Charles C Canver, MD, Ashvin K Patel, MD, Peter Kosolcharoen, MD, Marta C Voytovich,

Giant Cardiac Fibroma The Annals of Thoracic Surgery

Induction of Interleukin-19 and Interleukin-22 After Cardiac Surgery With Cardiopulmonary Bypass Chung-Hsi Hsing, MD, Mei-Yi Hsieh, MS, Wei-Yu Chen,

Deborah J. Kozik, DO, James S. Tweddell, MD

Model for endothelial infection and renal injury in severe, disseminated O. tsutsugamushi infection. Model for endothelial infection and renal injury in.

Regression of intracardiac heparin-induced thrombosis after aortic root surgery Frédéric Collart, MD, David Derouck, MD, Francois Kerbaul, MD, Horea Feier,

Guro Valen, MD, PhD, Gabrielle Paulsson, PhD, Jarle Vaage, MD, PhD

Gene transfection of hepatocyte growth factor attenuates the progression of cardiac remodeling in the hypertrophied heart Keiji Iwata, MD, Yoshiki Sawa,

Presentation transcript:

Gene expression of inflammatory mediators in different chambers of the human heart Guro Valen, MD, PhD, Gabrielle Paulsson, PhD, Anna M Bennet, Göran K Hansson, MD, PhD, Jarle Vaage, MD, PhD The Annals of Thoracic Surgery Volume 70, Issue 2, Pages 562-567 (August 2000) DOI: 10.1016/S0003-4975(00)01507-1

Fig 1 A polyacrylamide gel with radiolabelled reverse transcription-polymerase chain reaction (RT-PCR) products from cardiac tissue of a patient with unstable angina undergoing open heart surgery with cardiopulmonary bypass. Biopsies were taken simultaneously from the left atrium (LA), right atrium (RA), and left ventricle (LV) before cardioplegic arrest. Histone H3 (215 bp) was employed as a reference gene to evaluate the relative amount of test genes, which were tumor necrosis factor α (TNFα, 444 bp), interleukin-1β (IL-1β, 464 bp), intercellular adhesion molecule-1 (ICAM-1, 237 bp), E-selectin (CD62E, 255 bp), CD18 (379 bp), CD25 (312 bp), endothelin-1 (ET-1, 725 bp), inducible nitric oxide synthase (iNOS, 532 bp), and endothelial NOS (eNOS, 737 bp). The Annals of Thoracic Surgery 2000 70, 562-567DOI: (10.1016/S0003-4975(00)01507-1)

Fig 2 Gene expression of tumor necrosis factor α (TNFα), interleukin-1β (IL-1β), and intercellular adhesion molecule-1 (ICAM-1) in human heart biopsies from the right atrium (RA), left atrium (LA), and left ventricle (LV) obtained before cardioplegic arrest during open heart surgery. Reverse transcription-polymerase chain reaction (RT-PCR) was performed, band density measured, and the ratio between test gene and reference gene calculated (= relative mRNA). The left panels show mean ± SEM values from 19 patients, and the right panels show mean ± SEM of subgroups of patients with unstable angina (uCABG, n = 7), stable angina (CABG, n = 7), and valvular or combined disease (VR, n = 5). The Annals of Thoracic Surgery 2000 70, 562-567DOI: (10.1016/S0003-4975(00)01507-1)

Fig 3 Gene expression of E-selectin (CD62E), CD18, and CD25 in human heart biopsies from the right atrium (RA), left atrium (LA), and left ventricle (LV) obtained before cardioplegic arrest during open heart surgery (see text to Fig 2). The Annals of Thoracic Surgery 2000 70, 562-567DOI: (10.1016/S0003-4975(00)01507-1)

Fig 4 Gene expression of endothelin-1 (ET-1), inducible nitric oxide synthase (iNOS), and endothelial NOS (eNOS) in human heart biopsies (see text to Fig 2). The Annals of Thoracic Surgery 2000 70, 562-567DOI: (10.1016/S0003-4975(00)01507-1)