Nature vs. Nurture: Examining the Impact of a Manipulated Environment

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Presentation transcript:

Nature vs. Nurture: Examining the Impact of a Manipulated Environment Allison N. Frando*, Dr. Fidelma O’Leary, St. Edward’s University

Purpose Personal Interest Behavioral Neuroscience NPY-1 Neuropeptide Y Receptor Controls behavioral processes Inability to test human subjects

What are C. elegans? Microscopic nematodes 1mm in length Short lifespan Hermaphroditic NPR-1 Neuropeptide Receptor 1 Involved in aggregation and social behaviors Analogous to NPY-1 in humans

Can Environment Override Genetic Coding in C. elegans? Research Question Can Environment Override Genetic Coding in C. elegans?

Strains Transgenic strains: Feeding behaviors N2 Wildtype AX 301 Solitary High npr-1 activity SD 1891 Social Low npr-1 activity Feeding behaviors

Methods: Treatments Preferred Social Manipulated x8 Preferred x8 Solitary Manipulated

Lifespan Assay Age Synchronization FLDR Plates (8 Worms) Monitor Survival 4 HOURS

Lifespan Data: Social Strain Number of C. elegans Number of Days

Lifespan Data: Solitary Strain Number of C. elegans Number of Days

Fertility Assay Age synchronized Count Eggs Laid Day Five Count Eggs Laid Recounted larvae next day 4 HOURS

Fertility Assay Data

Anecdotal Data Social in preferred environment Social in manipulated environment

Conclusions Environment seems to override genetics Future Research Shorter Lifespan Decreased Fertility Future Research GFP – Quantification of Consumption Additional anecdotal data

Works Cited http://www.nature.com/nature/journal/v458/n7242/full/nature07886.html http://www.sciencedirect.com/science/article/pii/S0092867400816098 http://www.plosbiology.org/article/info%3Adoi%2F10.1371%2Fjournal.pbio.1001237 http://www.jove.com/video/3362/visualization-caenorhabditis-elegans-cuticular-structures-using http://www.plosgenetics.org/article/info%3Adoi%2F10.1371%2Fjournal.pgen.1004156 http://www.ncrna.org/glocal/cgi-bin/hgGene?org=C.+elegans&hgg_gene=C39E6.6&hgg_chrom=none&db=ce2

Acknowledgements Dr. Richard Kopec NSF STEP Grant #0969153 Dr. Fidelma O’Leary Olivia O’Keefe Cassandra Boduch Gilbert Rivera