Dominant Th1 and Minimal Th17 Skewing in Discoid Lupus Revealed by Transcriptomic Comparison with Psoriasis Ali Jabbari, Mayte Suárez-Fariñas, Judilyn.

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Dominant Th1 and Minimal Th17 Skewing in Discoid Lupus Revealed by Transcriptomic Comparison with Psoriasis Ali Jabbari, Mayte Suárez-Fariñas, Judilyn Fuentes-Duculan, Juana Gonzalez, Inna Cueto, Andrew G. Franks, James G. Krueger Journal of Investigative Dermatology Volume 134, Issue 1, Pages 87-95 (January 2014) DOI: 10.1038/jid.2013.269 Copyright © 2014 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 1 Transcriptomic analysis of discoid lupus erythematosus (DLE). (a) Selected top canonical pathways from interrogation of the DLE (n=7) versus normal transcriptome (n=13) with Ingenuity Pathway Analysis. (b) Quantitative reverse transcriptase–PCR (RT–PCR) of selected upregulated genes in the DLE versus normal transcriptome corresponding to IFN-associated genes (MX1, OASL, STAT1), immune cell–associated genes, and selected cytokines in DLE (n=7) and normal (n=6) samples. Genes were normalized to human ribosomal acidic protein. Box and whisker plots (middle line: median; box: lower to upper quartile; whiskers: minimum and maximum). *P<0.05, **P<0.005, ***P<5 × 10−4, ****P<5 × 10−5, †P<5 × 10−6, ††P<5 × 10−7, ††††P<5 × 10−9. DC, dendritic cell; NK, natural killer cell; PRR, pattern recognition receptor; RA, rheumatoid arthritis; SLE, systemic lupus erythematosus. Journal of Investigative Dermatology 2014 134, 87-95DOI: (10.1038/jid.2013.269) Copyright © 2014 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 2 Microscopic and immunohistologic comparison of discoid lupus erythematosus (DLE) and psoriasis. Skin sections from patients with DLE, psoriasis, or healthy control patients were stained with hematoxylin and eosin (H&E) or were subjected to immunohistochemistry with antibodies specific for CD3, CD11c, or CD8. Scale bar =100 μm. Journal of Investigative Dermatology 2014 134, 87-95DOI: (10.1038/jid.2013.269) Copyright © 2014 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 3 Transcriptomic comparison between discoid lupus erythematosus (DLE) and psoriasis. (a) Principal components (PC) analysis plot comparing DLE (n=7), psoriasis (n=18), and normal control (n=13) samples. (b) Venn diagram comparing upregulated and downregulated genes from DLE versus normal skin (DLE) and psoriasis versus normal skin. (c) Heat map comparing the most upregulated and downregulated genes in DLE versus psoriasis. Journal of Investigative Dermatology 2014 134, 87-95DOI: (10.1038/jid.2013.269) Copyright © 2014 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 4 Comparison of T helper subsets in discoid lupus erythematosus (DLE) and psoriasis. (a) Quantitative reverse transcriptase–PCR (RT–PCR) comparison of T helper subset–associated cytokines in DLE and psoriasis. *P<0.05, **P<5 × 10−5, †P<5 × 10−6. (b) Intracellular cytokine staining of CD4 T cells extracted from lesions from patients with DLE (n=4) or psoriasis (n=4) for IFN-γ or IL-17A and stimulated with (+PMA/I, right column) or without (-PMA/I, left column) phorbol myristate acetate ionomycin (PMA) and ionomycin (I). Numbers are percentages of CD3+CD4+ events positive for either IFN-γ or IL-17A. Journal of Investigative Dermatology 2014 134, 87-95DOI: (10.1038/jid.2013.269) Copyright © 2014 The Society for Investigative Dermatology, Inc Terms and Conditions