Quantification of cartilage oligomeric matrix protein (COMP) and a COMP neoepitope in synovial fluid of patients with different joint disorders by novel.

Slides:

Advertisements

Similar presentations

Efficacy of ultrasound therapy for the management of knee osteoarthritis: a systematic review with meta-analysis A. Loyola-Sánchez, J. Richardson, N.J.

Advertisements

Type II collagen C2C epitope in human synovial fluid and serum after knee injury – associations with molecular and structural markers of injury N. Kumahashi,

The association between metacarpal ratio, radiographic hand and knee osteoarthritis and its progression after meniscectomy P.T. Paradowski, L.S. Lohmander,

D. A. Walsh, F. R. C. P. , Ph. D. , C. S. Bonnet, B. Sc. , E. L

Serum hyaluronic acid as a potential marker with a predictive value for further radiographic progression of hand osteoarthritis M. Filková, L. Šenolt,

Western blot quantification of aggrecan fragments in human synovial fluid indicates differences in fragment patterns between joint diseases A. Struglics,

Development of a novel clinical biomarker assay to detect and quantify aggrecanase- generated aggrecan fragments in human synovial fluid, serum and urine

MicroRNA221-3p modulates Ets-1 expression in synovial fibroblasts from patients with osteoarthritis of temporomandibular joint J. Xu, Y. Liu, M. Deng,

H.J. Smith, Ph.D., J.B. Richardson, M.D., A. Tennant, Ph.D.

The epidemiology and impact of pain in osteoarthritis

J.L. Huebner, M.S., V.B. Kraus, M.D., Ph.D.

Validity and sensitivity to change of three scales for the radiographic assessment of knee osteoarthritis using images from the Multicenter Osteoarthritis.

Glucosamine but not ibuprofen alters cartilage turnover in osteoarthritis patients in response to physical training S.G. Petersen, T. Saxne, D. Heinegard,

The association between changes in synovial fluid levels of ARGS-aggrecan fragments, progression of radiographic osteoarthritis and self-reported outcomes:

An ARGS-aggrecan assay for analysis in blood and synovial fluid

P.-S. Hsu, H.-H. Lin, C.-R. Li, W.-S. Chung

Disability and quality of life of patients with knee or hip osteoarthritis in the primary care setting and factors associated with general practitioners'

Concentrations of synovial fluid biomarkers and the prediction of knee osteoarthritis 16 years after anterior cruciate ligament injury P. Neuman, L.E.

Aging-related inflammation in osteoarthritis

Chemokine profile of synovial fluid from normal, osteoarthritis and rheumatoid arthritis patients: CCL25, CXCL10 and XCL1 recruit human subchondral mesenchymal.

Associations between pre-operative radiographic changes and outcomes after total knee joint replacement for osteoarthritis M.M. Dowsey, M. Nikpour, P.

Osteoarthritis as a disease of mechanics

Changes in synovial fluid and serum concentrations of cartilage oligomeric matrix protein over five years after anterior cruciate ligament rupture A.

Quantitation OF ARGS aggrecan fragments in synovial fluid, serum and urine from osteoarthritis patients F.M. Germaschewski, C.J. Matheny, J. Larkin,

Lifetime body mass index, other anthropometric measures of obesity and risk of knee or hip osteoarthritis in the GOAL case-control study K.L. Holliday,

Tenascin-C levels in synovial fluid are elevated after injury to the human and canine joint and correlate with markers of inflammation and matrix degradation

D. A. Walsh, F. R. C. P. , Ph. D. , C. S. Bonnet, B. Sc. , E. L

Usefulness of specific OA biomarkers, Coll2-1 and Coll2-1NO2, in the anterior cruciate ligament OA canine model Y. Henrotin, J. Martel-Pelletier, P.

Quantitative genetic study of amphiregulin and fractalkine circulating levels – potential markers of arthropathies A. Leonov, S. Trofimov, S. Ermakov,

The progression of chronic kidney disease: A 10-year population-based study of the effects of gender and age B.O. Eriksen, O.C. Ingebretsen Kidney International

Measurement of matrilin-3 levels in human serum and synovial fluid using a competitive enzyme-linked immunosorbent assay J.-B. Vincourt, P. Gillet, A.-C.

D. R. Seifer, B. A. , B. D. Furman, M. S. , F. Guilak, Ph. D. , S. A

Serum adipokines in osteoarthritis; comparison with controls and relationship with local parameters of synovial inflammation and cartilage damage T.N.

Serum concentration of cartilage oligomeric matrix protein (COMP) is sensitive to physiological cyclic loading in healthy adults Annegret Mündermann,

Dr. M. C. Honorati, Ph. D. , S. Neri, Ph. D. , L. Cattini, B. Sc. , A

Time to be positive about negative data?

Human aggrecanase generated synovial fluid fragment levels are elevated directly after knee injuries due to proteolysis both in the inter globular and.

Resistin is elevated following traumatic joint injury and causes matrix degradation and release of inflammatory cytokines from articular cartilage in.

J. Ranstam Osteoarthritis and Cartilage

Elevated high-sensitivity C-reactive protein levels are associated with local inflammatory findings in patients with osteoarthritis A.D. Pearle, M.D.,

Spectrocolorimetric evaluation of human articular cartilage

Osteoarthritis of the knee after meniscal resection: long term radiographic evaluation of disease progression P.T. Paradowski, L.S. Lohmander, M. Englund

Utilization rates of hip arthroplasty in OECD countries

C. -H. Chou, H. -G. Wisniewski, P. Band, J. Huebner, T. Stabler, C

Risk factors for onset of osteoarthritis of the knee in older adults: a systematic review and meta-analysis M. Blagojevic, C. Jinks, A. Jeffery, K.P.

Activation of Indian hedgehog promotes chondrocyte hypertrophy and upregulation of MMP-13 in human osteoarthritic cartilage F. Wei, J. Zhou, X. Wei,

Who should have a joint replacement? A plea for more ‘phronesis’

Large scale meta-analysis of urinary C-terminal telopeptide, serum cartilage oligomeric protein and matrix metalloprotease degraded type II collagen and.

Evaluation of the inflammatory response in experimentally induced synovitis in the horse: a comparison of recombinant equine interleukin 1 beta and lipopolysaccharide

Interleukin-6 and tumor necrosis factor alpha in synovial fluid are associated with progression of radiographic knee osteoarthritis in subjects with previous.

D.J. Hunter Osteoarthritis and Cartilage

S.G. Muthuri, D.F. McWilliams, M. Doherty, W. Zhang

Preoperative progressive explosive-type resistance training is feasible and effective in patients with hip osteoarthritis scheduled for total hip arthroplasty.

Most patients gain weight in the 2 years after total knee arthroplasty: comparison to a healthy control group J.A. Zeni, L. Snyder-Mackler Osteoarthritis.

T. Silvestri, M. D. , L. Pulsatelli, Ph. D. , P. Dolzani, Ph. D. , A

Development and preliminary evaluation of the OsteoArthritis Questionnaire (OA- Quest): a psychometric study L. Busija, R. Buchbinder, R.H. Osborne Osteoarthritis.

Osteoarthritis year 2012 in review: biology

Volume 76, Issue 8, Pages (October 2009)

Location-specific hip joint space width for progression of hip osteoarthritis – Data from the Osteoarthritis Initiative C. Ratzlaff, C. Van Wyngaarden,

H. -G. Wisniewski, E. Colón, V. Liublinska, R. J. Karia, T. V

P. Jüni, N. Low, S. Reichenbach, P. M. Villiger, S. Williams, P. A

Interleukin-6 and tumor necrosis factor alpha in synovial fluid are associated with progression of radiographic osteoarthritis in subjects with previous.

Systematic review of the impact of osteoarthritis on health outcomes for comorbid disease in older people L. Parkinson, D.L. Waters, L. Franck Osteoarthritis.

Osteochondral injury increases type II collagen degradation products (C2C) in synovial fluid of Thoroughbred racehorses T.N. Trumble, D.V.M., Ph.D.,

Urine cartilage oligomeric matrix protein (COMP) measurement is useful in discriminating the osteoarthritic Thoroughbreds K. Misumi, Ph.D., M. Tagami,

The potential and limitations of cartilage-specific (V+C)− fibronectin and cartilage oligomeric matrix protein as osteoarthritis biomarkers in canine.

Synovial fluid concentration of gremlin1 but not frizzled-related protein is increased in patients after acute knee injury L. Zhong, J. Leijten, J. Post,

Measurement of synovial fluid volume using urea

A. Carlson, B. Bothner, R. June Osteoarthritis and Cartilage

General Information Osteoarthritis and Cartilage

Presentation transcript:

Quantification of cartilage oligomeric matrix protein (COMP) and a COMP neoepitope in synovial fluid of patients with different joint disorders by novel automated assays P. Lorenzo, A. Aspberg, T. Saxne, P. Önnerfjord Osteoarthritis and Cartilage Volume 25, Issue 9, Pages 1436-1442 (September 2017) DOI: 10.1016/j.joca.2017.04.004 Copyright © 2017 Osteoarthritis Research Society International Terms and Conditions

Fig. 1 Dose–response standard curve for A) Total COMP and B) COMP neoepitope-S77. Standard curves display RLUs. Inserted graph in A shows the Total COMP assay with the recombinant pentameric COMP (molecular mass 524 kDa; Image 1) and with the recombinant fragment of COMP (theoretical molecular mass pentameric structure 32 kDa; Image 2). Inserted graph in B shows the COMP neoepitope-S77 assay with the recombinant pentameric COMP (Image 1) and with the recombinant fragment of COMP (Image 2). A cartoon showing the location of the antibodies in the assays is shown. Osteoarthritis and Cartilage 2017 25, 1436-1442DOI: (10.1016/j.joca.2017.04.004) Copyright © 2017 Osteoarthritis Research Society International Terms and Conditions

Fig. 2 Synovial fluids from patients with joint diseases were measured for A) Total COMP assay and B) COMP neoepitope-S77 assay. C) Molar ratio COMP neoepitope-S77/Total COMP. Results shown are scatter plots of Total COMP and COMP neoepitope-S77 concentrations (log scale). Boxes represent the median and quartiles, whiskers the tenth and ninetieth percentiles. Significance values were obtained using log-transformed data by ANCOVA allowing adjustment for gender and age (differences were considered significant if P < 0.05). ReA (reactive arthritis, n = 18), RA (rheumatoid arthritis, n = 23), OA (osteoarthritis, n = 22), AT (acute trauma, n = 23). Osteoarthritis and Cartilage 2017 25, 1436-1442DOI: (10.1016/j.joca.2017.04.004) Copyright © 2017 Osteoarthritis Research Society International Terms and Conditions

Fig. 3 Molar ratio COMP neoepitope-S77/Total COMP analysis of measurement in synovial fluid from two patient groups with RA at different progression of joint destruction: RA0 no progression (Larsen grade 0–1); RA1 progression (joint replacement). Result shown is scatter plots of the ratio the values COMP neoepitope-S77/Total COMP (log scale). Boxes represent the median and quartiles, whiskers the tenth and ninetieth percentiles. Inserted table shows the median (25th–75th quartile). P values were obtained from the Mann–Whitney U test for group comparison (differences were considered significant if P < 0.05). Osteoarthritis and Cartilage 2017 25, 1436-1442DOI: (10.1016/j.joca.2017.04.004) Copyright © 2017 Osteoarthritis Research Society International Terms and Conditions