Changing substrate concentration

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Presentation transcript:

Changing substrate concentration Lesson 5

Success Criteria Describe and explain the effect of enzyme concentration and substrate concentration on enzyme activity

Starter Think, pair, share Effect of enzyme concentration on enzymes Effect of substrate concentration on enzymes

The Effect Of Substrate Concentration On The Rate Of Enzyme - Catalysed Reactions Low product concentration per unit time Low Substrate Concentration Increased Substrate Concentration More product formation; increased reaction rate

The Effect Of Substrate Concentration On The Rate Of Enzyme - Catalysed Reactions Maximum product formation; maximum rate of reaction Further increase in substrate concentration No further increase in product formation; maximum reaction rate maintained Excess substrate concentration Enzyme concentration is the LIMITING FACTOR

Enzymes and Substrate Concentration As SC increases there are more successful collisions and more enzyme substrate complexes form. Point of saturation: increase in SC has no effect, as all the active sites are occupied. Substrate molecules are having to ‘wait’ for empty active sites.

Effect of substrate concentration on rate

More on Substrate concentration... Initially, as substrate concentration increases, so does the rate of reaction. Why? More enzyme-substrate complexes can form. At this point, substrate concentration is the limiting factor. As substrate concentration rises further, the reaction will reach its maximum rate. As mentioned earlier, the point of saturation is reached as all active sites are occupied. What is now the limiting factor? Enzyme concentration.

Effect of enzyme concentration on rate

Success Criteria Describe and explain the effect of enzyme concentration and substrate concentration on enzyme activity