A new allergen family involved in pollen food-associated syndrome: Snakin/gibberellin- regulated proteins Hélène Sénéchal, PhD, Jiří Šantrůček, PhD, Magdalena.

Slides:

Advertisements

Similar presentations

Peter Vadas, MD, PhD, Boris Perelman, PhD, Gary Liss, MD, MS

Advertisements

Shea butter contains no IgE-binding soluble proteins

Identification of sesame seed allergens by 2-dimensional proteomics and Edman sequencing: Seed storage proteins as common food allergens Kirsten Beyer,

Allergy to latex, avocado pear, and banana: Evidence for a 30 kd antigen in immunoblotting François Lavaud, MD, Alain Prevost, MD, Claude Cossart, MD,

Identification and isolation of a Fel d 1–like molecule as a major rabbit allergen Christiane Hilger, PhD, Stéphanie Kler, MSc, Karthik Arumugam, PhD,

Lupine allergy: Not simply cross-reactivity with peanut or soy

Identification and immunologic characterization of an allergen, alliin lyase, from garlic (Allium sativum) Shao-Hsuan Kao, MS, Ching-Hsian Hsu, MD, PhD,

Wheat α-amylase inhibitor: A second route of allergic sensitization

Santa Jeremy Ono, BA, PhD, Mark B. Abelson, MD

Giant ragweed specific immunotherapy is not effective in a proportion of patients sensitized to short ragweed: Analysis of the allergenic differences.

Plantago lanceolata: An important trigger of summer pollinosis with limited IgE cross- reactivity Gabriele Gadermaier, PhD, Stephanie Eichhorn, MSc, Eva.

Delineating the specificity of an IgE-encoding transcriptome

Christiane Kruse Fæste, PhD, Ellen Namork, PhD, Helene Lindvik, MMSc

Identification of the cysteine protease Amb a 11 as a novel major allergen from short ragweed Julien Bouley, PhD, Rachel Groeme, MSc, Maxime Le Mignon,

Act d 12 and Act d 13: Two novel, masked, relevant allergens in kiwifruit seeds Sofía Sirvent, PhD, Belén Cantó, BSc, Javier Cuesta-Herranz, MD, PhD,

Correlation of IgE/IgG4 milk epitopes and affinity of milk-specific IgE antibodies with different phenotypes of clinical milk allergy Julie Wang, MD,

Molecular cloning, expression, and characterization of a major 38-kd cochineal allergen Yoko Ohgiya, MS, Fumihiro Arakawa, MS, Hiroshi Akiyama, PhD, Yasuo.

Persistence of the IgE repertoire in birch pollen allergy

Sabine Fischer, MSc,a, Monika Grote, PhD,b, B. Fahlbusch, PhD,c, W. D

Diagnosing IgE-mediated hypersensitivity to sesame by an immediate-reading “contact test” with sesame oil Cristiana Alonzi, MD, Paolo Campi, MD, Francesco.

Is 9 more than 2 also in allergic airway inflammation?

Rapid and comprehensive discovery of unreported shellfish allergens using large-scale transcriptomic and proteomic resources Roni Nugraha, MSc, Sandip.

Allergy to eggplant (Solanum melongena)

Shea butter contains no IgE-binding soluble proteins

Allergy testing in predicting outcome of open food challenge to peanut

Anaphylactic reaction to (mixed) fruit juice containing dragon fruit

Major cat and dog allergens share IgE epitopes

ImmunoCAP cellulose displays cross-reactive carbohydrate determinant (CCD) epitopes and can cause false-positive test results in patients with high anti-CCD.

Lipid transfer protein (Ara h 9) as a new peanut allergen relevant for a Mediterranean allergic population Susanne Krause, PhD, Gerald Reese, PhD, Stefanie.

Respiratory sensitization of a worker to the warehouse beetle Trogoderma variabile: An index case report Jonathan A. Bernstein, MD, Marjorie S. Morgan,

The brown marmorated stinkbug as a new aeroallergen

Genetically engineered hybrid proteins from Parietaria judaica pollen for allergen- specific immunotherapy Roberto González-Rioja, PhD, Ignacio Ibarrola,

Cross-reactivity between Cupressus arizonica and Cupressus sempervirens pollen extracts Bianca Barletta, BSc, Claudia Afferni, BSc, Raffaella Tinghino,

Sol i 1, the phospholipase allergen of imported fire ant venom

Food allergy: A review and update on epidemiology, pathogenesis, diagnosis, prevention, and management Scott H. Sicherer, MD, Hugh A. Sampson, MD Journal.

Biosimilars and drug development in allergic and immunologic diseases

Peter Vadas, MD, PhD, Boris Perelman, PhD, Gary Liss, MD, MS

IgE-mediated anaphylaxis caused by bites of the pigeon tick Argas reflexus: Cloning and expression of the major allergen Arg r 1 Christiane Hilger, PhD,

Identification of 2 new sesame seed allergens: Ses i 6 and Ses i 7

Weiguo Chen, PhD, Gurjit K. Khurana Hershey, MD, PhD

Casein-related anaphylaxis after use of an Everlast kickboxing glove

Molecular cloning and expression in insect cells of honeybee venom allergen acid phosphatase (Api m 3) Thomas Grunwald, PhD, Benjamin Bockisch, PhD,

Jason B. Price, MD, Adnan Divjan, William R. Montfort, PhD, Kirstie H

Cockroach allergens: Coping with challenging complexity

Autophagy: Nobel Prize 2016 and allergy and asthma research

Food allergy: Epidemiology, pathogenesis, diagnosis, and treatment

Anaphylaxis to apple and orange seed

Advances in the approach to the patient with food allergy

Identification of a polygalacturonase as a major allergen (Pla a 2) from Platanus acerifolia pollen Ignacio Ibarrola, PhD, M. Carmen Arilla, PhD, Alberto.

Prevention of food allergy: Beyond peanut

α-Purothionin, a new wheat allergen associated with severe allergy

Lipid transfer protein (Ara h 9) as a new peanut allergen relevant for a Mediterranean allergic population Susanne Krause, PhD, Gerald Reese, PhD, Stefanie.

Recombinant allergen-based IgE testing to distinguish bee and wasp allergy Irene Mittermann, PhD, Mihaela Zidarn, MD, Mira Silar, PhD, Zora Markovic-Housley,

Fungal protein MGL_1304 in sweat is an allergen for atopic dermatitis patients Takaaki Hiragun, MD, PhD, Kaori Ishii, MSc, Makiko Hiragun, MD, Hidenori.

Allergy to human seminal fluid: Cross-reactivity with dog dander

Cloning, sequencing, and recombinant production of Sin a 2, an allergenic 11S globulin from yellow mustard seeds Oscar Palomares, PhD, Andrea Vereda,

Pedro Gamboa, MD, PhD, Rosa Sanchez-Monge, PhD, M

Peanut oral immunotherapy decreases IgE to Ara h 2 and Ara h 6 but does not enhance sensitization to cross-reactive allergens Riikka Uotila, MD, Anna.

Profilin (Che a 2) and polcalcin (Che a 3) are relevant allergens of Chenopodium album pollen: Isolation, amino acid sequences, and immunologic properties

Cloning, expression, and clinical significance of the major allergen from ash pollen, Fra e 1 Rodrigo Barderas, BSc, Ashok Purohit, MD, Ioanna Papanikolaou,

Teresa Asensio, MD, Jesus F

Identification of wheat gliadins as an allergen family related to baker's asthma Cordula Bittner, MD, Britta Grassau, MS, Karsten Frenzel, PhD, Xaver.

Gastrointestinal digestion of Bet v 1–homologous food allergens destroys their mediator- releasing, but not T cell–activating, capacity Eva Maria Schimek,

Placental transfer of allergen-specific IgG but not IgE from a specific immunotherapy– treated mother Sabine Flicker, PhD, Katharina Marth, MD, Heinz.

Personal and parental nativity as risk factors for food sensitization

Hymenoptera venom protease allergens

The use of serum-specific IgE measurements for the diagnosis of peanut, tree nut, and seed allergy Jennifer M. Maloney, MD, Magnus Rudengren, BSc, Staffan.

Carrier-bound, nonallergenic Ole e 1 peptides for vaccination against olive pollen allergy Teresa E. Twaroch, MSc, Margit Focke, PhD, Vera Civaj, Milena.

The effects of gastric digestion on codfish allergenicity

Natural history of cow’s milk allergy

Presentation transcript:

A new allergen family involved in pollen food-associated syndrome: Snakin/gibberellin- regulated proteins Hélène Sénéchal, PhD, Jiří Šantrůček, PhD, Magdalena Melčová, MS, Petr Svoboda, PhD, Jarmila Zídková, PhD, Denis Charpin, MD, Laurence Guilloux, PhD, Youcef Shahali, PhD, Marie-Ange Selva, ME, Rémy Couderc, PhD, Tomoyasu Aizawa, PhD, Pascal Poncet, PhD Journal of Allergy and Clinical Immunology Volume 141, Issue 1, Pages 411-414.e4 (January 2018) DOI: 10.1016/j.jaci.2017.06.041 Copyright © 2017 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 1 A, Mass spectrometry analysis of in-gel–separated BP14 proteins from Cupressus sempervirens pollen extract. The gel was run under reducing condition. For detailed information, see this article's Methods section. B, Sequence alignment of peamaclein from Prunus persica (P86888), snakin-1 from Solanum tuberosum, and GRP from Theobroma cacao (GRP Theo, A0A061FGF7). The enlighted sequences correspond to the peptides identified in mass spectrometry analysis. MW, Molecular weight; pI, isoelectric point. SC (%) percent coverage. *Amino acid identity with peamaclein sequence. †Snakin-1 sequence without signal peptide as described in Kuddus et al.4 Journal of Allergy and Clinical Immunology 2018 141, 411-414.e4DOI: (10.1016/j.jaci.2017.06.041) Copyright © 2017 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 2 IgE immunoreactivity of BP14+ (n = 15) and BP14− (n = 15) CPAP tested on cypress pollen extract (A) or snakin-1 (B). Total cypress proteins are silver stained (Fig 2, A) and snakin-1 is stained with Coomassie blue (Fig 2, B). Detailed procedures are described in the Methods section. Molecular masses are indicated in kDa. C, IgE immunoreactivity is abolished when snakin-1 or BP14 is reduced. Left part corresponds to silver-stained total proteins, and right part to IgE immunoreactivity using serum number 7 from a BP14+ CPAP. D, Crossed inhibition using a “depletion” protocol. NCa-blotted snakin or NCa-blotted cypress pollen extract (Cups) strips were incubated with either unadsorbed or snakin-adsorbed or cypress pollen extract–adsorbed (Cups) CPAP serum number 14 as indicated and IgE binding was revealed. Total proteins (Total prot) were stained either with Coomassie blue (snakin-1) or with silver nitrate (Cups). Cups, Cupressus sempervirens pollen extract; +, alkylation with iodoacetamide. Molecular masses are indicated in kDa. Journal of Allergy and Clinical Immunology 2018 141, 411-414.e4DOI: (10.1016/j.jaci.2017.06.041) Copyright © 2017 American Academy of Allergy, Asthma & Immunology Terms and Conditions