Localization of angiotensin II, the AT1 receptor, angiotensin-converting enzyme, aminopeptidase A, adipocyte-derived leucine aminopeptidase, and vascular.

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Localization of angiotensin II, the AT1 receptor, angiotensin-converting enzyme, aminopeptidase A, adipocyte-derived leucine aminopeptidase, and vascular endothelial growth factor in the human ovary throughout the menstrual cycle Toko Harata, M.D., Hisao Ando, M.D., Akira Iwase, M.D., Tetsuro Nagasaka, M.D., Shigehiko Mizutani, M.D., Fumitaka Kikkawa, M.D. Fertility and Sterility Volume 86, Issue 2, Pages 433-439 (August 2006) DOI: 10.1016/j.fertnstert.2006.01.041 Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions

FIGURE 1 Immunohistochemical localization of Ang II (A–E), ACE (F–J), APA (K–N), and A-LAP (O) in human ovarian tissues displaying immunostaining with various intensities in association with (A) primordial follicles, (B, F, K) mature follicles, (C) luteal tissue from the early luteal phase, (D, G, L, O) luteal tissues from the midluteal phase, (E, H, M) luteal tissues from the late luteal phase, (I, N) luteal tissues at early pregnancy, and (J) corpus albicans. Po = primordial follicle; St = stroma of the ovarian cortex; Gr = granulosa cells; Th = theca cells; Ll = large luteal cells; Sl = small luteal cells; Pe = pericytes. Original magnification; ×200. Bar in (O) = 100 μm. Harata. Ang II-related proteins in human ovary. Fertil Steril 2006. Fertility and Sterility 2006 86, 433-439DOI: (10.1016/j.fertnstert.2006.01.041) Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions

FIGURE 2 Immunohistochemical localization of AT1R (A–G), VEGF (H–M), and negative controls using mouse IgG (N) or rabbit IgG (O) in human ovarian tissues displaying immunostaining with various intensities in association with (A–C, H) primordial follicles, (B, I) primary follicles, (C, D, J, N) secondary/mature follicles, (E, K, O) luteal tissues from the midluteal phase, (F, L) luteal tissues from the late luteal phase, and (G, M) luteal tissues from the late luteal phase. Po = primordial follicle; Gr = granulosa cells; Th = theca cells; En = endothelial cells; Ll = large luteal cells; Sl = small luteal cells. Original magnification; ×200. Bar in (O) = 100 μm. Harata. Ang II-related proteins in human ovary. Fertil Steril 2006. Fertility and Sterility 2006 86, 433-439DOI: (10.1016/j.fertnstert.2006.01.041) Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions