IRE1 signaling signatures in glioblastoma multiform

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Fig. 4. Positive expression of GD2 and CD90 were significantly increased in glioblastoma multiforme (GBM) cells than neural stem cells (NSCs). BD Lyoplate.

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IRE1 signaling signatures in glioblastoma multiform IRE1 signaling signatures in glioblastoma multiform AFunctional annotations of the IRE1 gene expression signature identified in U87 cells (Pluquet et al, 2013).BSchematic representation of the analysis workflow.CHierarchical clustering of GBM patients (TCGA cohort) based on high or low IRE1 activity as assessed with the correlation index of their median z‐score with the expression pattern of the IRE1 gene signature of 38 hub genes (see Materials and Methods and Appendix Fig S1). Pearson correlation was used to measure the similarity between different genes and tumor cases, as well. The correlation index refers to the gene expression median z‐score with (+) or (−) sign for identical or reverse expression pattern with that of WT vs. DN, respectively. The expression pattern of WT vs. DN has been described in detail in Pluquet et al (2013). Blue: low correlation index, red: high correlation index.DSurvival analysis of the GBM patients exhibiting high (red) or low (green) IRE1 activity. Student's t‐test was used with Welch's correction when SD different.E–GExpression of microglial/monocyte/macrophage (IBA1, CD14, CD163) (E), angiogenesis (CD31, vWF) (F), and migration/invasion (RHOA, CYR61, CTGF) (G) markers mRNA in the IRE1high (red) and IRE1low (green) populations. Probe analysis was carried out in data from 258 and 265 tumors in IRE1high and IRE1low groups, respectively. Horizontal lines indicate median; box lines indicate first & third quartiles; whiskers indicate min & max. Student's t‐test was used with Welch's correction when SD different.HFACS analysis of CD45/CD11b in freshly dissociated GBM tumors exhibiting high or low IRE1 activity.IFACS analysis of CD31 in freshly dissociated GBM tumors exhibiting high or low IRE1 activity. In both cases, 7AAD was used to exclude dead cells.JRelative distribution of the different classes of GBM—pro‐neural (blue), neural (orange), classic (green), and mesenchymal (red) according to the tumor status, namely IRE1high or IRE1low. Stéphanie Lhomond et al. EMBO Mol Med. 2018;emmm.201707929 © as stated in the article, figure or figure legend