Transplantation of frozen–thawed late-gestational-age human fetal ovaries into immunodeficient mice Ronit Abir, Ph.D., Tal Biron-Shental, M.D., Raoul Orvieto, M.D., M.M.Sc., Roni Garor, M.Sc., Haim Krissi, M.D., Benjamin Fisch, M.D., Ph.D. Fertility and Sterility Volume 92, Issue 2, Pages 770-777 (August 2009) DOI: 10.1016/j.fertnstert.2008.06.032 Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions
Figure 1 Photographs of fetal ovarian sections at various stages of the experiment. (A) Section of ovary from a 28-GW-old fetus with cardiac abnormalities, PROH and sucrose frozen–thawed. Note the red-brown PCNA staining in the oocytes and stroma cells. Original magnification ×400. (B) Section of ovary from 22-GW-old fetus with brain abnormalities, PROH and sucrose frozen–thawed after 3.5 months of grafting. Note the multiple secondary follicles with the red-brown PCNA staining in the GCs (arrows). Original magnification ×100. (C) Section of ovary from the same fetus as in (A), after 6 months of grafting. Note the antral follicle (400 μm in diameter). Hematoxylin and eosin, original magnification ×400. (D) Section of ovary from a 24-GW-old fetus, DMSO frozen–thawed, after 5 days of grafting. Note the net-like hollows. The ovarian graft is devoid of follicles. Hematoxylin and eosin, original magnification ×400. (E) Section of ovary from a 33-GW-old fetus, DMSO frozen–thawed, after 6 months of grafting. Note the tubule-like structures and lack of follicles. Hematoxylin and eosin, original magnification ×400. Fertility and Sterility 2009 92, 770-777DOI: (10.1016/j.fertnstert.2008.06.032) Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions