Development of selective media for the isolation of yeasts and filamentous fungi from the sputum of adult patients with cystic fibrosis (CF) Yuriko Nagano,

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Presentation transcript:

Development of selective media for the isolation of yeasts and filamentous fungi from the sputum of adult patients with cystic fibrosis (CF) Yuriko Nagano, B. Cherie Millar, Colin E. Goldsmith, James M. Walker, J. Stuart Elborn, Jackie Rendall, John E. Moore Journal of Cystic Fibrosis Volume 7, Issue 6, Pages 566-572 (November 2008) DOI: 10.1016/j.jcf.2008.06.007 Copyright © 2008 Elsevier B.V. Terms and Conditions

Fig. 1 Comparative growth of a pure culture of Exophiala dermatitidis on SDA, SDA+ and Medium B+. +, basal medium supplemented with cotrimethoxazole, 128 mg/l; chloramphenicol, 50 mg/l; ceftazidime, 32 mg/l; colistin, 24 mg/l. To view this figure in full colour, please go to the on-line version. Journal of Cystic Fibrosis 2008 7, 566-572DOI: (10.1016/j.jcf.2008.06.007) Copyright © 2008 Elsevier B.V. Terms and Conditions

Fig. 2 Comparison of colonial morphologies of yeasts and filamentous fungi isolated from the sputum of an adult CF patient with SDA, SDA+ and Medium B+. +, basal medium supplemented with cotrimethoxazole, 128 mg/l; chloramphenicol, 50 mg/l; ceftazidime, 32 mg/l; colistin, 24 mg/l. To view this figure in full colour, please go to the on-line version. Journal of Cystic Fibrosis 2008 7, 566-572DOI: (10.1016/j.jcf.2008.06.007) Copyright © 2008 Elsevier B.V. Terms and Conditions

Fig. 3 Demonstration of bacterial overgrowth on SDA and unsupplemented Medium by Pseudomonas aeruginosa, from a sputum of an adult patient with CF. Note how supplementation of Medium B+ with the antibiotic combination (cotrimethoxazole, 128 mg/l; chloramphenicol, 50 mg/l; ceftazidime, 32 mg/l; colistin, 24 mg/l), inhibits the bacterial co-flora in the sputum and allows the proliferation of yeast and filamentous colonial morphologies. To view this figure in full colour, please go to the on-line version. Journal of Cystic Fibrosis 2008 7, 566-572DOI: (10.1016/j.jcf.2008.06.007) Copyright © 2008 Elsevier B.V. Terms and Conditions