Rat and human aortic smooth muscle cells display differing migration and matrix metalloproteinase activities in response to dexamethasone C. Pross, MD,

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Presentation transcript:

Rat and human aortic smooth muscle cells display differing migration and matrix metalloproteinase activities in response to dexamethasone C. Pross, MD, M.M. Farooq, MD, J.S. Lane, MD, N. Angle, MD, C.K. Tomono, A.E. Xavier, MS, J.A. Freischlag, MD, A.E. Collins, PhD, R.E. Law, PhD, H.A. Gelabert, MD Journal of Vascular Surgery Volume 35, Issue 6, Pages 1253-1259 (June 2002) DOI: 10.1067/mva.2002.123332 Copyright © 2002 Society for Vascular Surgery and The American Association for Vascular Surgery Terms and Conditions

Fig. 1 Smooth muscle cell migration. Dexamethasone inhibits rat aortic smooth muscle cell migration in dose-dependent fashion (n = 9). Dexamethasone does not alter human aortic smooth muscle cell migration (n = 9). *P <.05. Journal of Vascular Surgery 2002 35, 1253-1259DOI: (10.1067/mva.2002.123332) Copyright © 2002 Society for Vascular Surgery and The American Association for Vascular Surgery Terms and Conditions

Fig. 2 Apoptosis. Dexamethasone at 10−9 mol/L to 10−5 mol/L does not induce apoptosis of rat or human smooth muscle cells. Cell survival was uniform throughout all groups, as determined with flow cytometry (n = 3). Journal of Vascular Surgery 2002 35, 1253-1259DOI: (10.1067/mva.2002.123332) Copyright © 2002 Society for Vascular Surgery and The American Association for Vascular Surgery Terms and Conditions

Fig. 3 MMP-2 secretion. Dexamethasone inhibits rat aortic smooth muscle cell MMP-2 secretion in dose-dependent manner (n = 3). Top, Western blot immunoassay. Densitometry (below). Dexamethasone does not effect human aortic smooth muscle cell MMP-2 secretion (n = 3). Bottom, Western blot immunoassay. Densitometry (below). *P <.05. Journal of Vascular Surgery 2002 35, 1253-1259DOI: (10.1067/mva.2002.123332) Copyright © 2002 Society for Vascular Surgery and The American Association for Vascular Surgery Terms and Conditions

Fig. 4 MMP-2 activity. Dexamethasone inhibits rat aortic smooth muscle cell MMP-2 activity in dose-dependent manner (n = 3). Top, Zymogram. Densitometry (below). Dexamethasone does not alter human aortic smooth muscle cell MMP-2 activity (n = 3). Bottom, Zymogram. Densitometry (below). *P <.05. Journal of Vascular Surgery 2002 35, 1253-1259DOI: (10.1067/mva.2002.123332) Copyright © 2002 Society for Vascular Surgery and The American Association for Vascular Surgery Terms and Conditions

Fig. 5 TIMP-2 secretion. Dexamethasone activates rat aortic smooth muscle cell TIMP-2 secretion in dose-dependent manner (n = 3). Top, Western blot immunoassay. Densitometry (below). Dexamethasone does not alter TIMP-2 secretion (n = 3). Bottom, Western blot immunoassay. Densitometry (below). *P <.05. Journal of Vascular Surgery 2002 35, 1253-1259DOI: (10.1067/mva.2002.123332) Copyright © 2002 Society for Vascular Surgery and The American Association for Vascular Surgery Terms and Conditions