ΑB-Crystallin Improves Murine Cardiac Function and Attenuates Apoptosis in Human Endothelial Cells Exposed to Ischemia-Reperfusion Jeffrey B. Velotta,

Slides:

Advertisements

Similar presentations

Paracrine Action Enhances the Effects of Autologous Mesenchymal Stem Cell Transplantation on Vascular Regeneration in Rat Model of Myocardial Infarction

Advertisements

Accelerated aneurysmal dilation associated with apoptosis and inflammation in a newly developed calcium phosphate rodent abdominal aortic aneurysm model

Reduced hind limb ischemia-reperfusion injury in Toll-like receptor-4 mutant mice is associated with decreased neutrophil extracellular traps Rahmi Oklu,

Ulka Sachdev, MD, Xiangdong Cui, MS, Edith Tzeng, MD

Sirtuin1 Regulates the Stem Cell Therapeutic Effects on Regenerative Capability for Treating Severe Heart Failure in a Juvenile Animal Model Hideto Ozawa,

Diabetes Upregulation of Cyclooxygenase 2 Contributes to Altered Coronary Reactivity After Cardiac Surgery Jun Feng, MD, PhD, Kelsey Anderson, Arun K.

Acidosis-induced apoptosis in human and porcine heart

Role of Toll-Like Receptor-4 in Lung Ischemia-Reperfusion Injury

Mary E. Huerter, MD, MA, Ashish K

Long-Term Outcome of Coronary Artery Bypass Grafting in Patients With Left Ventricular Dysfunction Paolo Nardi, MD, PhD, Antonio Pellegrino, MD, Antonio.

Faraz Kerendi, MD, Paul M. Kirshbom, MD, Michael E

Jennifer Tomich, MD, Mohit Bhasin, MD, Jonathan Philpott, MD

Naoyuki Kimura, MD, Susumu Nakae, PhD, Satoshi Itoh, MD, Denis R

Paracrine Action Enhances the Effects of Autologous Mesenchymal Stem Cell Transplantation on Vascular Regeneration in Rat Model of Myocardial Infarction

Salah Sabbagh, MD, Michele M. Henry Salzman, BS, Robert A

Left Ventricular Assist Device Design Reduces von Willebrand Factor Degradation: A Comparative Study Between the HeartMate II and the EVAHEART Left Ventricular.

Functional Roles of Tumor Necrosis Factor-Alpha and Interleukin 1-Beta in Hypoxia and Reoxygenation Heather E. Merry, MD, Patrick Phelan, MD, Matthew.

Pulmonary Fibroblasts Induce Epithelial Mesenchymal Transition and Some Characteristics of Stem Cells in Non-Small Cell Lung Cancer Yasushi Shintani,

Ulka Sachdev, MD, Xiangdong Cui, MS, Edith Tzeng, MD

The Annals of Thoracic Surgery

Extent of Aortic Coverage and Incidence of Spinal Cord Ischemia After Thoracic Endovascular Aneurysm Repair Robert J. Feezor, MD, Tomas D. Martin, MD,

Cytokines Link Toll-Like Receptor 4 Signaling to Cardiac Dysfunction After Global Myocardial Ischemia John Cha, MD, Zhiping Wang, MD, Lihua Ao, BS, Ning.

Comparison of Three Measurements of Cardiac Surgery Mortality for the Northern New England Cardiovascular Disease Study Group Donald S. Likosky, PhD,

Value of Magnetic Resonance Imaging, Angiography, and Fractional Flow Reserve to Evaluate the Left Main Coronary Artery After Direct Surgical Angioplasty

Nathalie Roy, MD, Ingeborg Friehs, MD, Douglas B

Coronary Bypass Grafting to a “Full-Metal Jacket” Left Anterior Descending Artery María J. Mataró, MD, Gemma Sánchez-Espín, MD, Carlos Porras, MD, José.

The Journal of Thoracic and Cardiovascular Surgery

Sevoflurane Attenuates Ischemia-Reperfusion Injury in a Rat Lung Transplantation Model Akihiro Ohsumi, MD, Katherine Marseu, MD, Peter Slinger, MD, Karen.

Down-Regulation of Apoptosis After Left Ventricular Aneurysm Repair

Pseudoaneurysm of an Aortic Homograft

Injectable Small Intestine Submucosal Extracellular Matrix in an Acute Myocardial Infarction Model Hadi Daood Toeg, MD, MS, Rashmi Tiwari-Pandey, PhD,

Toll-Like Receptor 4 Mediates Lung Ischemia-Reperfusion Injury

Therapeutic Potential of Human Umbilical Cord Derived Stem Cells in a Rat Myocardial Infarction Model Kai Hong Wu, MD, PhD, Bin Zhou, PhD, Cun Tao Yu,

Interleukin-6 Regulation of Direct Lung Ischemia Reperfusion Injury

Nicolas H. Pope, MD, Morgan Salmon, PhD, William F

Sarah M. Brant, MD, Michael L. Cobert, PhD, LaShondra M

Proteasome Inhibition Attenuates Infarct Size and Preserves Cardiac Function in a Murine Model of Myocardial Ischemia-Reperfusion Injury William E. Stansfield,

Surgical Resection of a Giant Coronary Aneurysm

Susanne Lison, MD, Michael Spannagl, MD, PhD, Wulf Dietrich, MD, PhD

Comparison of Systemic and Retrograde Delivery of Adenosine A2A Agonist for Attenuation of Spinal Cord Injury After Thoracic Aortic Cross-Clamping T.

Initial Clinical Experience With the Symphony Heart Assist System

Cardioplegia and Diazoxide Modulate STAT3 Activation and DNA Binding

Protection by 20-5,14-HEDGE Against Surgically Induced Ischemia Reperfusion Lung Injury in Rats Irshad Ali, PhD, Stephanie Gruenloh, BS, Ying Gao, MS,

Surgical Dilemmas: Diagnosis and Treatment of Atrial Plasmacytoma

Anomalous Origin of Left Pulmonary Artery From Left Subclavian Artery

Long Segmental Reconstruction of Diffusely Diseased Left Anterior Descending Coronary Artery With Left Internal Thoracic Artery With or Without Endarterectomy

Coronary Artery Fistulae Involving Both Right and Left Coronary Arteries Ahmet Bolukcu, MD, Ahmet Can Topcu, MD, Yavuz Sensoz, MD, Ilyas Kayacioglu, MD

Amanda M. Marshall, MD, Mitchell I. Cohen, MD, Ashish B

Xianyao Xu, MS, Jennifer L. Philip, MD, Md

Chadrick E. Denlinger, MD, Brian K. Rundall, DO, Michael D

Noninvasive Assessment of Brain Injury in a Canine Model of Hypothermic Circulatory Arrest Using Magnetic Resonance Spectroscopy Christopher J. Barreiro,

Sarah C. Huen, MD, Chirag R. Parikh, MD, PhD

Aortic Regurgitation With Markedly Reduced Left Ventricular Function Is Not a Contraindication for Aortic Valve Replacement Tsuyoshi Kaneko, MD, Julius.

Completely Thrombosed Tricuspid Pouch Mimicking a Cardiac Tumor

Daniel P. Mulloy, MD, Ashish K. Sharma, MBBS, PhD, Lucas G

Anirudh Vinnakota, MS, Robert Stewart, MD, Gosta Pettersson, MD, PhD

Off-Pump Plication of Left Ventricular Aneurysm

Acute Esophageal Necrosis: A Case Series and Long-Term Follow-Up

Survival After Isolated Coronary Artery Bypass Grafting in Patients With Severe Left Ventricular Dysfunction Waleed A. Ahmed, MD, Phillip J. Tully, BHSc(Hons),

Age- and Gender-Related Differences in Mitochondrial Oxygen Consumption and Calcium With Cardioplegia and Diazoxide James D. McCully, PhD, Anthony J.

Invited Commentary The Annals of Thoracic Surgery

Catheter-Based Endoscopic Bypass Grafting: An Experimental Feasibility Study Stephan Jacobs, MD, David Holzhey, MD, Hubert Stein, BS, BME, Friedrich W.

Adam R. Williams, MD, Andrew Wang, MD, Han Kim, MD, Carmelo A

Y. Joseph Woo, MD, Michael A. Acker, MD The Annals of Thoracic Surgery

A Giant Cavernous Hemangioma of the Heart

Effects of in vivo myocardial ischemia and reperfusion on interstitial nitric oxide metabolites Randy M Stevens, MD, M.Salik Jahania, MD, Jennifer E.

The Impact of New Technology on Cardiothoracic Surgical Practice

Karl A. Bocchieri, BS, CCP, S. Jacob Scheinerman, MD, L

David H. Harpole, MD, Stanley A. Gall, MD, Walter G. Wolfe, MD, J

Michael J. Weyant, MD, Manjit S

Presentation transcript:

αB-Crystallin Improves Murine Cardiac Function and Attenuates Apoptosis in Human Endothelial Cells Exposed to Ischemia-Reperfusion Jeffrey B. Velotta, MD, Naoyuki Kimura, MD, Stephanie H. Chang, BS, Jaehoon Chung, MD, Satoshi Itoh, MD, Jonathan Rothbard, PhD, Philip C. Yang, MD, Lawrence Steinman, MD, Robert C. Robbins, MD, Michael P. Fischbein, MD, PhD The Annals of Thoracic Surgery Volume 91, Issue 6, Pages 1907-1913 (June 2011) DOI: 10.1016/j.athoracsur.2011.02.072 Copyright © 2011 The Society of Thoracic Surgeons Terms and Conditions

Fig 1 The C57BL/6 mice underwent temporary left anterior descending artery (LAD) occlusion for 30 minutes. Phosphate-buffered saline (PBS) or αB-crystallin (CryAB) was administered intramyocardially 15 minutes before reperfusion of the LAD (PBS 100 μL; CryAB 100 μL, 50 μg) and after ischemia-reperfusion (I/R) injury (PBS 100 μL; CryAB 100 μL, 50 μg) every other day, by intraperitoneal injection. Left ventricular ejection fraction (LVEF) was measured by magnetic resonance imaging (%) of PBS-treated and CryAB-treated C57BL/6 mice 40 days after I/R injury (n = 6 per group; *p < 0.005). Data are mean ± SD. The Annals of Thoracic Surgery 2011 91, 1907-1913DOI: (10.1016/j.athoracsur.2011.02.072) Copyright © 2011 The Society of Thoracic Surgeons Terms and Conditions

Fig 2 Human microvascular endothelial cells (HMEC-1) were incubated with 50 μg αB-crystallin (CryAB), 500 μg/10 mL, or phosphate-buffered saline (PBS) in a hypoxia chamber for 24 hours and then allowed to reoxygenate at room air for 30 minutes. (A) Representative Western blots of PBS-treated or CryAB-treated HMEC-1 cells. (B) Enzyme-linked immunosorbent assay cytoplasmic histone-associated DNA fragment apoptosis assay of PBS-treated or CryAB-treated HMEC-1 cells (†p < 0.01). (C) Caspase-3 activity assay of PBS-treated or CryAB-treated (50 μg) HMEC-1 cells (*p < 0.005). (OD = optical densitometry.) The Annals of Thoracic Surgery 2011 91, 1907-1913DOI: (10.1016/j.athoracsur.2011.02.072) Copyright © 2011 The Society of Thoracic Surgeons Terms and Conditions

Fig 3 Densitometric analysis of Western blot in human microvascular endothelial cells (HMEC-1), in vitro. Quantitative densitometric analysis of Western blots were performed in HMEC-1 treated with αB-crystallin (CryAB) 10 μg (light gray bars) or 50 μg (dark gray bars) or phosphate-buffered saline (PBS [black bars]) after different hypoxia times (6 hours, 12 hours, and 24 hours) and then allowed to reoxygenate at room air for 30 minutes. Protein was normalized with β-actin signal. (Each treatment group, n = 3 to 4.) (A) Bcl-2; (B) free bax; (C) cleaved caspase-3; (D) cleaved caspase-9; (E) cleaved-PARP. (NS = not significant.) The Annals of Thoracic Surgery 2011 91, 1907-1913DOI: (10.1016/j.athoracsur.2011.02.072) Copyright © 2011 The Society of Thoracic Surgeons Terms and Conditions

Fig 4 Mouse HL-1 murine atrial cardiomyocytes were incubated with 50 μg αB-crystallin (CryAB) 500 μg/10 mL, or phosphate-buffered saline (PBS) in a hypoxia chamber (1% O2, 5% CO2, 94% N2) for 24 hours and then allowed to reoxygenate at room air for 30 minutes. (A) Representative Western blots of PBS-treated or CryAB-treated HL-1 cells. (B) Enzyme-linked immunosorbent assay cytoplasmic histone-associated DNA fragment apoptosis assay of PBS-treated or CryAB-treated HL-1 cells. (C) Caspase-3 activity assay of PBS-treated or CryAB-treated HL-1 cells. (OD = optical densitometry.) The Annals of Thoracic Surgery 2011 91, 1907-1913DOI: (10.1016/j.athoracsur.2011.02.072) Copyright © 2011 The Society of Thoracic Surgeons Terms and Conditions