Transcription and Translation

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Presentation transcript:

Transcription and Translation 2-4,6,8-9,12-17,21-22

Protein Structure Made up of amino acids Polypeptide- 20 amino acids are arranged in different orders to make a variety of proteins _____________________ribosome

Replication DNA double helix unwinds DNA now New DNA strand forms using complementary base pairing (A-T, C-G) Whole genome copied/replicated

Transcription and Translation: An Overview (aka the _______________)

RNA vs. DNA DNA RNA Double stranded Single stranded Deoxyribose sugar Bases: C,G A,T RNA Single stranded Ribose sugar Bases: C,G,A,U

Transcription RNA forms base pairs with DNA Primary transcript- C-G A-U Primary transcript-

TRANSCRIPTION ACGATACCCTGACGAGCGTTAGCTATCG UGC GGG

Major players in transcription mRNA-

Major players in transcription RNA polymerase- complex of enzymes with 2 functions:

Transcription is done…what now? Now we have mature mRNA transcribed from the cell’s DNA. It is leaving the nucleus through a nuclear pore. Once in the cytoplasm, it finds a ribosome so that translation can begin. We know how mRNA is made, but how do we “read” the code?

Translation mRNA is on a ribosome

Ribosomes 2 subunits, separate in cytoplasm until they join to begin translation Contain 3 binding sites

Translation Second stage of protein production mRNA is on a ribosome

tRNA Transfer RNA Bound to one amino acid on one end Anticodon

tRNA Function tRNA lines up amino acids using mRNA code

Reading the DNA code Every DNA bases pairs with mRNA bases Every group of mRNA bases encodes a single amino acid Codon-

The Genetic Code

Which codons code for which amino acids? Genetic code- A gene

Transcription vs. Translation Review Process by which genetic information encoded in DNA is copied onto messenger RNA Occurs in the nucleus DNA mRNA Translation Process by which information encoded in mRNA is used to assemble a protein at a ribosome Occurs on a Ribosome mRNA protein