Note the clone IDs used in the report duplicates are F176 and R551/F146 and F220 (see slides 10 and 11) CRN8 F118 F220 F283 R514 F146 F176 R582 R551.

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Note the clone IDs used in the report duplicates are F176 and R551/F146 and F220 (see slides 10 and 11) CRN8 F118 F220 F283 R514 F146 F176 R582 R551

Report of Cahid Cakir on CRN8 deletions This was the basis for Figure 2 of van Damme et al. PLOS Pathogens 2012

Crn8 constructs (deletion and mutation) Targeting Function SP LFLAK Effector GDV CRN8 1 17 49 439 599 F118 118 F118-M1 118 ??? F146 146 F176 176 F220 220 F283 283 R582 118 582 R551 118 551 R514 118 514

Crn8 constructs (Full Length) Targeting Function SP LFLAK Effector CRN8 1 17 49 439 599 Crn8 PGR106

F118-Crn8 constructs without signal peptide and LFLAK Targeting Function SP LFLAK Effector CRN8 1 17 49 439 599 F118 118 PGR106 F118 Crn2

F283-Crn8 constructs Targeting Function LFLAK CRN8 F283 PGR106 F118 SP LFLAK Effector DN CRN8 1 17 49 439 470 475 599 DN F283 283 PGR106 F118 F220 F283

F220-Crn8 constructs Targeting Function LFLAK CRN8 F220 PGR106 F118 SP LFLAK Effector DN CRN8 1 17 49 439 470 475 599 DN F220 220 PGR106 F118 F220 F283

R514-Crn8 constructs without signal peptide and LFLAK Targeting Function SP LFLAK Effector CRN8 1 17 49 439 599 R514 118 439 R514 F118

New Deletion Analysis of Crn8

F118, F146 & F147 F118 118 F146 146 F176 176 F146 F118 F176

F118, F220 & R551 F118 118 F220 220 R551 118 551 F220 F118 R551

F118 & R582 F118 118 R582 118 582 R582 F118

Summary of results – from Cahid’s report

F118, F220 & R551 F118 118 F220 220 R551 118 551 F220 F118 R551