Autoradiographic mapping and characterization of insulin-like growth factor-I receptor binding in human greater saphenous vein Anton N. Sidawy, MD, Fares.

Slides:

Advertisements

Similar presentations

Endothelial cell seeding reduces thrombogenicity of Dacron grafts in humans Per Örtenwall, MD, PhD *, Hans Wadenvik, MD, PhD **, Jack Kutti, MD, PhD **,

Advertisements

The role of arteriovenous shunts in the pathogenesis of varicose veins Howard C. Baron, M.D., Sebastiano Cassaro, M.D. Journal of Vascular Surgery Volume.

The effect of inguinal lymphatic manipulation on regional lymph flow patterns Jeffrey R. Rubin, MD, Lisa B. Eberlin, MD Journal of Vascular Surgery Volume.

Aortic injury occurring after minor trauma in ankylosing spondylitis

Axillary-to-carotid artery bypass grafting for symptomatic severe common carotid artery occlusive disease Joseph P. Archie, PhD, MD Journal of Vascular.

Heparin inhibits thrombin-induced mitogen-activated protein kinase signaling in arterial smooth muscle cells Ulf Hedin, MD, PhD, Günter Daum, PhD, Alexander.

Lloyd M. Taylor, M.D., Edward S. Phinney, M.D., John M. Porter, M.D.

Insulin action enhancement normalizes brachial artery vasoactivity in patients with peripheral vascular disease and occult diabetes Ricardo Avena, MD,

Fetal internal iliac artery: Case report and embryologic review

Scott D. Gitlin, MD, Michael Deeb, MD, Christopher Yann, BS, Alvin H

Mycotic axillary artery aneurysm

A new valvulotome for in situ bypass grafts

Aortic injury occurring after minor trauma in ankylosing spondylitis

Right internal thoracic artery or saphenous vein grafting

The effect of valvulotomy on the flow rate through the saphenous vein graft: Clinical implications Albert K. Chin, MD, David N. Mayer, BA, Robert K.

Flush saphenofemoral ligation and multiple stab phlebectomy preserve a useful greater saphenous vein four years after surgery Louis J. Fligelstone, FRCS,

Malcolm O. Perry, MD, Richard Kempczinski, MD

Recurrent thoracic outlet syndrome

Characterization of endothelin receptors in human varicose veins

James E. Chapman, M.D., William Allen Loy, M.D.

Anton N. Sidawy, MD, Fares S. Hakim, MD, Bruce A. Jones, MD, Jose M

Thomas E. Brothers, MD, Jacob G. Robison, MD, Bruce M

Peripheral atherectomy with the rotablator: A multicenter report

Todd K. Rosengart, M. D. , John P. Kupferschmid, M. D. , Victor J

Bao-Ngoc Nguyen, MD, Richard F

Objective tinnitus resulting from internal carotid artery stenosis

The Society for Vascular Surgery: Built to last

Regarding "Flush saphenofemoral ligation and multiple stab phlebectomy preserve a useful greater saphenous vein four years after surgery" Germano Lucertini,

Unusual venous thrombosis associated with protein C deficiency

Reviewer Acknowledgment

Angioscope-assisted occlusion of venous tributaries with prolamine in in situ femoropopliteal bypass: Preliminary results of canine experiments John.

A. N. Nicolaides, MS, FRCS, M. K. Hussein, MD, FRCS, G. Szendro, MD, D

Functional analysis of cryopreserved veins

Calvin B. Ernst, MD Journal of Vascular Surgery

An improved retraction system for vascular surgical procedures

Anthony D. Whittemore, MD, Magruder C. Donaldson, MD, John A

John Hunter—triumph and tragedy

Cardiovascular surgery—The rocket and its stars: Presidential address

The fate of the donor artery in extraanatomic revascularization

Composite sequential bypasses to the ankle and beyond for limb salvage

Research and research funding in vascular surgery

Regarding “Venous ulcers and the superficial venous system”

J.Dennis Baker, MD Journal of Vascular Surgery

Septic embolism complicating infective endocarditis

Polytetrafluoroethylene versus human umbilical vein in above-knee femoropopliteal bypass: Six-year results of a randomized clinical trial Gijs J. Aalders,

Arie Markel, MD, Richard A. Manzo, BS, CCVT, Robert O. Bergelin, MS, D

Seizures following subclavian-carotid bypass

Jay B. Fisher, MD, Marc A. Granson, MD Journal of Vascular Surgery

Richard J. Sanders, MD 1, William H. Pearce, MD 2

Should patient age be a consideration in carotid endarterectomy?

Acute aortic occlusion – Factors that influence outcome

Monitoring vascular surgical performance

Glenn C. Hunter, M.D., Stan N. Carson, M.D.

Carotid endarterectomy for nonhemispheric cerebral symptoms: Patient selection with ocular pneumoplethysmography Kenneth Ouriel, M.D., John J. Ricotta,

Aortoesophageal fistula: Case report and literature review

Homologous veins as an arterial substitute: Long-term results

D. Emerick Szilagyi, MD—An appreciation

Presidential address: The second-generation vascular surgeon

Richard F. Kempczinski, M.D. Journal of Vascular Surgery

George D. Lilly 1906–1988 Journal of Vascular Surgery

James A. DeWeese, MD Journal of Vascular Surgery

Jerry Goldstone, MD Journal of Vascular Surgery

Axillary-to-carotid artery bypass grafting for symptomatic severe common carotid artery occlusive disease Joseph P. Archie, PhD, MD Journal of Vascular.

Innominate artery trauma: A thirty-year experience

Popliteal entrapment syndrome: A report of tibial nerve entrapment

Supraclavicular reoperation for neurogenic thoracic outlet syndrome

Thigh claudication due to profunda femoris artery occlusion

Lessons learned in adopting the in situ saphenous vein bypass

Endovascular repair of two abdominal aortic aneurysms

Cornelius A. Sullivan, MD, Michael J. Rohrer, MD, Bruce S. Cutler, MD

Presentation transcript:

Autoradiographic mapping and characterization of insulin-like growth factor-I receptor binding in human greater saphenous vein Anton N. Sidawy, MD, Fares S. Hakim, MD, Richard F. Neville, MD, Louis Y. Korman, MD Journal of Vascular Surgery Volume 18, Issue 6, Pages 947-954 (December 1993) DOI: 10.1016/0741-5214(93)90549-2 Copyright © 1993 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 1 A, Hematoxylin-cosin-stained frozen section of human greater saphenous vein. B, Autoradiograph of adjacent section demonstrating total 125I-IGF-I grain density corresponding to vein wall. Optical density of binding measures 0.37 ± 0.04. C, Nonspecific binding for insulin. Excess of insulin was used to compete with 125I-IGF-I for its binding sites. Optical density measures 0.15 ± 0.01. D, Nonspecific binding for IGF-I. Excess of unlabeled IGF-I was used to compete with 125I-IGF-I for binding sites. Optical density measures 0.05 ± 0.007, which is not different from optical density of film measured at 0.04 ± 0.004. Note difference in nonspecific binding between C and D. Difference in optical density of C and D autoradiographs is statistically significant with p < 0.005 with paired t test. Journal of Vascular Surgery 1993 18, 947-954DOI: (10.1016/0741-5214(93)90549-2) Copyright © 1993 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 2 Scatchard plot of 125I-IGF-I bound/free as function of bound radiolabeled peptide. Each data point represents mean ± SEM of results of veins studied. Calculated Kd = 1 ± 0.32 nmol/L and Bmax = 3.64 ± 0.47 pmol/L (0.46 ± 0.23 pmol/mg protein). Journal of Vascular Surgery 1993 18, 947-954DOI: (10.1016/0741-5214(93)90549-2) Copyright © 1993 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 3 Binding inhibition curves of IGF-I and insulin. Each value is represented as mean ± SEM of results obtained from veins studied. Curves were plotted between 125I-IGF-I bound as percentage of maximal binding and concentration of unlabeled IGF-I and insulin used to compete with 125I-IGF-I binding. Calculated IC50 for IGF-I and insulin were 0.069 μmol/L and 6.9 μmol/L, respectively. Journal of Vascular Surgery 1993 18, 947-954DOI: (10.1016/0741-5214(93)90549-2) Copyright © 1993 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions