M.M. Francou, J. Ten, R. Bernabeu, J. De Juan

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Presentation transcript:

Capacitation and acrosome reaction changes α-tubulin immunodistribution in human spermatozoa M.M. Francou, J. Ten, R. Bernabeu, J. De Juan Reproductive BioMedicine Online Volume 28, Issue 2, Pages 246-250 (February 2014) DOI: 10.1016/j.rbmo.2013.10.001 Copyright © 2013 Reproductive Healthcare Ltd. Terms and Conditions

Figure 1 Immunolocaization of α-tubulin in human spermatozoa. Confocal micrographs shows that in some spermatozoa, α-tubulin was located only in the flagellum end part (A–C), while in others it was detected all over the flagellum (D–F). Bars=5μm. Reproductive BioMedicine Online 2014 28, 246-250DOI: (10.1016/j.rbmo.2013.10.001) Copyright © 2013 Reproductive Healthcare Ltd. Terms and Conditions

Figure 2 Cluster sperm distribution. Heat maps and dendrograms provided visual representations of the distribution of spermatozoa in fresh, capacitated and acrosome-reacted samples according to the α-tubulin-labelled area (α-TLA). (For interpretation to colors in this figure, the reader is referred to the web version of this paper.) Reproductive BioMedicine Online 2014 28, 246-250DOI: (10.1016/j.rbmo.2013.10.001) Copyright © 2013 Reproductive Healthcare Ltd. Terms and Conditions

Figure 3 Quantification of cluster sperm distribution. In cluster A (α-tubulin-labelled area ⩾66.66%), the sperm percentage increased in capacitated and acrosome-reacted versus fresh samples, while in clusters B (α-tubulin-labelled area 33.33–66.66%) and C (α-tubulin-labelled area ⩽33.33%), this percentage decreased in capacitated and acrosome-reacted versus fresh samples. Reproductive BioMedicine Online 2014 28, 246-250DOI: (10.1016/j.rbmo.2013.10.001) Copyright © 2013 Reproductive Healthcare Ltd. Terms and Conditions