Long-term liquid nitrogen vapor storage of mouse embryos cryopreserved using vitrification or slow cooling Jin Hee Eum, M.Sc., Jae Kyun Park, M.Sc.,

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Long-term liquid nitrogen vapor storage of mouse embryos cryopreserved using vitrification or slow cooling Jin Hee Eum, M.Sc., Jae Kyun Park, M.Sc., Woo Sik Lee, M.D., Ph.D., Kwang Ryul Cha, M.D., Tae Ki Yoon, M.D., Dong Ryul Lee, Ph.D. Fertility and Sterility Volume 91, Issue 5, Pages 1928-1932 (May 2009) DOI: 10.1016/j.fertnstert.2008.02.126 Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions

Figure 1 Cross-section of LN2 vapor tank. (A) LN2 vapor; (B) LN2. Fertility and Sterility 2009 91, 1928-1932DOI: (10.1016/j.fertnstert.2008.02.126) Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions

Figure 2 Comparison of results obtained using LN2 and LN2 vapor storage in mouse embryos frozen by vitrification or slow cooling. (A) Survival rate after vitrification/warming. (B) Blastocyst formation rate after vitrification/warming. (C) Survival rate after slow-cooled/thawing. (D) Blastocyst formation rate after slow-cooled/thawing. (E) Percent of apoptotic cells in blastocysts after warming. (F) Total number of cells in blastocysts after warming. 1W = 1 week, 1M = 1 month, 6M = 6 months. Fertility and Sterility 2009 91, 1928-1932DOI: (10.1016/j.fertnstert.2008.02.126) Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions