Suppression of natural killer cells in the presence of CD34+ blood progenitor cells and peripheral blood lymphocytes  J. Clausen, M. Enk, B. Vergeiner, K. Eisendle, A.L. Petzer, G. Gastl, E. Gunsilius  Biology of Blood and Marrow Transplantation  Volume 10, Issue 10, Pages 691-697 (October 2004) DOI: 10.1016/j.bbmt.2004.06.009 Copyright © 2004 American Society for Blood and Marrow Transplantation Terms and Conditions

Figure 1 A, Expansion (mean ± SEM) of CD56+ cells from PB of healthy females (control, n = 6), and from PBPC collections from patients in whom <100 CD34+ cells/μL PB (n = 5) or >100 CD34+ cells/μL (n = 5) were measured at the day of apheresis. *P = .08 versus control (ns); **P <.005 versus control (nonparametric Mann-Whitney test for unpaired samples). B, Expansion (mean ± SEM) of NK cells within bulk PBL cultures in the presence or absence of autologous CD34+ PBPCs (n = 10 for each experimental set). *P <.05; **P <.005 compared with PBL cultures without CD34+ cells (nonparametric Wilcoxon test for matched pairs). Biology of Blood and Marrow Transplantation 2004 10, 691-697DOI: (10.1016/j.bbmt.2004.06.009) Copyright © 2004 American Society for Blood and Marrow Transplantation Terms and Conditions

Figure 2 Proportion of (A) IL-2—, (B) IFN-γ—, and (C) IL-4—producing CD8+ T cells (solid bars) and CD4+ T cells (hatched bars), after incubation without or with autologous CD34+ cells, respectively (n = 7). D, IL-10 concentrations (pg/mL; mean ± SEM) in supernatants from purified T cells, purified CD34+ cells, and from co-cultures of T cells and autologous CD34+ cells (n = 6). P values are indicated in cases of significant differences. Biology of Blood and Marrow Transplantation 2004 10, 691-697DOI: (10.1016/j.bbmt.2004.06.009) Copyright © 2004 American Society for Blood and Marrow Transplantation Terms and Conditions

Figure 3 Purified CD34+ cells were cultured with autologous NK cells and T cells for 1 week. The percent CFU-GM recovery (ie, the CFU-GM outcome after 1 week in proportion to the input CFU-GM numbers) is given as mean ± SEM (n = 5; P = ns; Wilcoxon matched-pairs test). Biology of Blood and Marrow Transplantation 2004 10, 691-697DOI: (10.1016/j.bbmt.2004.06.009) Copyright © 2004 American Society for Blood and Marrow Transplantation Terms and Conditions